Significance of tagI and mfd genes in the virulence of non-typeable Haemophilus influenzae

Denis A. Spricigo; Pilar Cortes; David Moranta; Jordi Barbe; Jose Antonio Bengoechea; Montserrat Llagostera

doi:10.2436/20.1501.01.218

Significance of tagI and mfd genes in the virulence of non-typeable Haemophilus influenzae

Denis A. Spricigo
, Pilar Cortes
, David Moranta
, Jordi Barbe
, Jose Antonio Bengoechea
, Montserrat Llagostera^*

^*Corresponding author for this work

School of Medicine, Dentistry and Biomedical Sciences

Research output: Contribution to journal › Article › peer-review

1 Citation (Scopus)

194 Downloads (Pure)

Abstract

Non-typeable Haemophilus influenzae (NTHi) is an opportunist pathogen well adapted to the human upper respiratory tract and responsible for many respiratory diseases. In the human airway, NTHi is exposed to pollutants, such as alkylating agents, that damage its DNA. In this study, we examined the significance of genes involved in the repair of DNA alkylation damage in NTHi virulence. Two knockout mutants, tagI and mfd, encoding N(3)methyladenine-DNA glycosylase I and the key protein involved in transcription-coupled repair, respectively, were constructed and their virulence in a BALB/c mice model was examined. This work shows that N-3-methyladenine-DNA glycosylase I is constitutively expressed in NTHi and that it is relevant for its virulence.

Original language	English
Pages (from-to)	159-164
Number of pages	6
Journal	International Microbiology
Volume	17
Issue number	3
DOIs	https://doi.org/10.2436/20.1501.01.218
Publication status	Published - 2014

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This output contributes to the following UN Sustainable Development Goals (SDGs)

SDG 3 Good Health and Well-being

Keywords

Haemophilus influenzae
alkylating agents
virulence
genes tagI and mfd
DNA GLYCOSYLASE-I
ESCHERICHIA-COLI
ALKYLATING-AGENTS
REPAIR
DAMAGE

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10.2436/20.1501.01.218

Signifi cance of tagI and mfd genes in the virulence of non-typeable Haemophilus infl uenzae
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http://revistes.iec.cat/index.php/IM/article/view/136530

Cite this

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title = "Significance of tagI and mfd genes in the virulence of non-typeable Haemophilus influenzae",

abstract = "Non-typeable Haemophilus influenzae (NTHi) is an opportunist pathogen well adapted to the human upper respiratory tract and responsible for many respiratory diseases. In the human airway, NTHi is exposed to pollutants, such as alkylating agents, that damage its DNA. In this study, we examined the significance of genes involved in the repair of DNA alkylation damage in NTHi virulence. Two knockout mutants, tagI and mfd, encoding N(3)methyladenine-DNA glycosylase I and the key protein involved in transcription-coupled repair, respectively, were constructed and their virulence in a BALB/c mice model was examined. This work shows that N-3-methyladenine-DNA glycosylase I is constitutively expressed in NTHi and that it is relevant for its virulence.",

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T1 - Significance of tagI and mfd genes in the virulence of non-typeable Haemophilus influenzae

AU - Spricigo, Denis A.

AU - Cortes, Pilar

AU - Moranta, David

AU - Barbe, Jordi

AU - Bengoechea, Jose Antonio

AU - Llagostera, Montserrat

PY - 2014

Y1 - 2014

N2 - Non-typeable Haemophilus influenzae (NTHi) is an opportunist pathogen well adapted to the human upper respiratory tract and responsible for many respiratory diseases. In the human airway, NTHi is exposed to pollutants, such as alkylating agents, that damage its DNA. In this study, we examined the significance of genes involved in the repair of DNA alkylation damage in NTHi virulence. Two knockout mutants, tagI and mfd, encoding N(3)methyladenine-DNA glycosylase I and the key protein involved in transcription-coupled repair, respectively, were constructed and their virulence in a BALB/c mice model was examined. This work shows that N-3-methyladenine-DNA glycosylase I is constitutively expressed in NTHi and that it is relevant for its virulence.

AB - Non-typeable Haemophilus influenzae (NTHi) is an opportunist pathogen well adapted to the human upper respiratory tract and responsible for many respiratory diseases. In the human airway, NTHi is exposed to pollutants, such as alkylating agents, that damage its DNA. In this study, we examined the significance of genes involved in the repair of DNA alkylation damage in NTHi virulence. Two knockout mutants, tagI and mfd, encoding N(3)methyladenine-DNA glycosylase I and the key protein involved in transcription-coupled repair, respectively, were constructed and their virulence in a BALB/c mice model was examined. This work shows that N-3-methyladenine-DNA glycosylase I is constitutively expressed in NTHi and that it is relevant for its virulence.

KW - Haemophilus influenzae

KW - alkylating agents

KW - virulence

KW - genes tagI and mfd

KW - DNA GLYCOSYLASE-I

KW - ESCHERICHIA-COLI

KW - ALKYLATING-AGENTS

KW - REPAIR

KW - DAMAGE

U2 - 10.2436/20.1501.01.218

DO - 10.2436/20.1501.01.218

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JO - International Microbiology

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IS - 3

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Significance of tagI and mfd genes in the virulence of non-typeable Haemophilus influenzae

Abstract

UN SDGs

Keywords

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