Simultaneous determination of aflatoxins and ochratoxin a in food using a fully automated immunoaffinity column clean-up and liquid chromatography- fluorescence detection

D. Chan, S. J. MacDonald, V. Boughtflower, P. Brereton

Research output: Contribution to journalArticle

90 Citations (Scopus)

Abstract

An automated HPLC method for the simultaneous detection of aflatoxins (AF) and ochratoxin A (OA) has been developed. The method uses an immunoaffinity column containing antibodies specific to both AF and OA. The samples were extracted with an acetonitrile/water mixture and diluted with phosphate buffer saline (PBS). The aqueous extracts were then transferred to an ASPEC HPLC system for automated clean-up using AflaOchra™ immunoaffinity columns. OA and AF were quantified using HPLC with fluorescence detection, with a run time of approximately 40 min. Limits of quantification were estimated as 0.2 μg/kg for OA and AFB 1, AFB 2, AFG 1 and AFG 2. Initial validation of this method gave average recoveries of 72-101% for OA and AF for a range of food products (maize cereal products and peanut butter). Within laboratory RSD r and RSD R for a 5.0 μg/kg spike level in maize cereals was found to be 7.6-10.1% (AF and OA) and 10.2-13.8%, respectively.

Original languageEnglish
Pages (from-to)13-16
Number of pages4
JournalJournal of Chromatography A
Volume1059
Issue number1-2
DOIs
Publication statusPublished - 03 Dec 2004
Externally publishedYes

Keywords

  • Aflatoxins
  • Cereals
  • Food analysis
  • Mycotoxins
  • Nuts
  • Ochratoxin A

ASJC Scopus subject areas

  • Analytical Chemistry
  • Biochemistry
  • Organic Chemistry

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