Single locus inheritance and joint segregation analysis of minisatellite (VNTR) DNA loci in brown trout (Salmo trutta L.)

Paulo A. Prodöhl*, John B. Taggart, Andrew Ferguson

*Corresponding author for this work

Research output: Contribution to journalArticle

33 Citations (Scopus)

Abstract

Eleven minisatellite (VNTR) DNA loci were examined for Mendelian inheritance and possible linkage relationships in a brown trout family (54 offspring). Analyses of the full progeny set (males and females combined) showed no statistically significant departures from Mendelian expectations at any locus, and no new mutations were observed. However, at one locus, Str-A9, differential segregation (P < 0.001) of parental alleles into male and female progeny was recorded. This is indicative of tight linkage between Str-A9 and the sex determining region in the brown trout, and confirms the male as the heterogametic sex in this species. Joint segregation analyses of 83 different pairwise comparisons of loci revealed nonrandom assortment in only two instances, i.e. both male and female comparisons of Str-A22/l with Str-A22/2. These findings illustrate the importance of inheritance studies in clarifying the genetic basis underlying minisatellite DNA polymorphisms prior to their use as individual, familial or population markers.

Original languageEnglish
Pages (from-to)556-566
Number of pages11
JournalHeredity
Volume73
Issue number5
DOIs
Publication statusPublished - 01 Jan 1994

Keywords

  • Brown trout
  • Inheritance
  • Linkage
  • Minisatellite DNA
  • Salmo trutta
  • VNTR

ASJC Scopus subject areas

  • Genetics
  • Genetics(clinical)

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