Solution structure, membrane interactions, and protein binding partners of the tetraspanin Sm-TSP-2, a vaccine antigen from the human blood fluke schistosoma mansoni

Xinying Jia; Leigh Schulte; Alex Loukas; Darren Pickering; Mark Pearson; Mehdi Mobli; Alun Jones; Karl J. Rosengren; Norelle L. Daly; Geoffrey N. Gobert; Malcolm K. Jones; David J. Craik; Jason Mulvenna

doi:10.1074/jbc.M113.531558

Solution structure, membrane interactions, and protein binding partners of the tetraspanin Sm-TSP-2, a vaccine antigen from the human blood fluke schistosoma mansoni

Xinying Jia, Leigh Schulte, Alex Loukas, Darren Pickering, Mark Pearson, Mehdi Mobli, Alun Jones, Karl J. Rosengren, Norelle L. Daly, Geoffrey N. Gobert, Malcolm K. Jones, David J. Craik, Jason Mulvenna^*

^*Corresponding author for this work

Research output: Contribution to journal › Article › peer-review

37 Citations (Scopus)

Abstract

Background: Schistosome tetraspanin Sm-TSP-2 is a vaccine antigen.

Results: We describe the structure of the large extracellular domain of Sm-TSP-2, develop a model of its interactions with Tetraspanin-enriched-microdomain proteins and plasma membrane, and identify TEM constituents.

Conclusion: Structural conservation of the domain means this model is likely applicable to TSPs in general.

Significance: Tetraspanin-enriched-microdomain proteins provide further targets for multiplex vaccines and/or novel drug targets.

Original language	English
Pages (from-to)	7151-7163
Number of pages	13
Journal	Journal of Biological Chemistry
Volume	289
Issue number	10
Early online date	15 Jan 2014
DOIs	https://doi.org/10.1074/jbc.M113.531558
Publication status	Published - 07 Mar 2014
Externally published	Yes

ASJC Scopus subject areas

Biochemistry
Cell Biology
Molecular Biology

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Jia, X., Schulte, L., Loukas, A., Pickering, D., Pearson, M., Mobli, M., Jones, A., Rosengren, K. J., Daly, N. L., Gobert, G. N., Jones, M. K., Craik, D. J., & Mulvenna, J. (2014). Solution structure, membrane interactions, and protein binding partners of the tetraspanin Sm-TSP-2, a vaccine antigen from the human blood fluke schistosoma mansoni. Journal of Biological Chemistry, 289(10), 7151-7163. https://doi.org/10.1074/jbc.M113.531558

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title = "Solution structure, membrane interactions, and protein binding partners of the tetraspanin Sm-TSP-2, a vaccine antigen from the human blood fluke schistosoma mansoni",

abstract = "Background: Schistosome tetraspanin Sm-TSP-2 is a vaccine antigen. Results: We describe the structure of the large extracellular domain of Sm-TSP-2, develop a model of its interactions with Tetraspanin-enriched-microdomain proteins and plasma membrane, and identify TEM constituents. Conclusion: Structural conservation of the domain means this model is likely applicable to TSPs in general. Significance: Tetraspanin-enriched-microdomain proteins provide further targets for multiplex vaccines and/or novel drug targets.",

author = "Xinying Jia and Leigh Schulte and Alex Loukas and Darren Pickering and Mark Pearson and Mehdi Mobli and Alun Jones and Rosengren, {Karl J.} and Daly, {Norelle L.} and Gobert, {Geoffrey N.} and Jones, {Malcolm K.} and Craik, {David J.} and Jason Mulvenna",

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doi = "10.1074/jbc.M113.531558",

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Jia, X, Schulte, L, Loukas, A, Pickering, D, Pearson, M, Mobli, M, Jones, A, Rosengren, KJ, Daly, NL, Gobert, GN, Jones, MK, Craik, DJ & Mulvenna, J 2014, 'Solution structure, membrane interactions, and protein binding partners of the tetraspanin Sm-TSP-2, a vaccine antigen from the human blood fluke schistosoma mansoni', Journal of Biological Chemistry, vol. 289, no. 10, pp. 7151-7163. https://doi.org/10.1074/jbc.M113.531558

TY - JOUR

T1 - Solution structure, membrane interactions, and protein binding partners of the tetraspanin Sm-TSP-2, a vaccine antigen from the human blood fluke schistosoma mansoni

AU - Jia, Xinying

AU - Schulte, Leigh

AU - Loukas, Alex

AU - Pickering, Darren

AU - Pearson, Mark

AU - Mobli, Mehdi

AU - Jones, Alun

AU - Rosengren, Karl J.

AU - Daly, Norelle L.

AU - Gobert, Geoffrey N.

AU - Jones, Malcolm K.

AU - Craik, David J.

AU - Mulvenna, Jason

PY - 2014/3/7

Y1 - 2014/3/7

N2 - Background: Schistosome tetraspanin Sm-TSP-2 is a vaccine antigen. Results: We describe the structure of the large extracellular domain of Sm-TSP-2, develop a model of its interactions with Tetraspanin-enriched-microdomain proteins and plasma membrane, and identify TEM constituents. Conclusion: Structural conservation of the domain means this model is likely applicable to TSPs in general. Significance: Tetraspanin-enriched-microdomain proteins provide further targets for multiplex vaccines and/or novel drug targets.

AB - Background: Schistosome tetraspanin Sm-TSP-2 is a vaccine antigen. Results: We describe the structure of the large extracellular domain of Sm-TSP-2, develop a model of its interactions with Tetraspanin-enriched-microdomain proteins and plasma membrane, and identify TEM constituents. Conclusion: Structural conservation of the domain means this model is likely applicable to TSPs in general. Significance: Tetraspanin-enriched-microdomain proteins provide further targets for multiplex vaccines and/or novel drug targets.

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U2 - 10.1074/jbc.M113.531558

DO - 10.1074/jbc.M113.531558

M3 - Article

C2 - 24429291

AN - SCOPUS:84896800798

SN - 0021-9258

VL - 289

SP - 7151

EP - 7163

JO - Journal of Biological Chemistry

JF - Journal of Biological Chemistry

IS - 10

ER -

Solution structure, membrane interactions, and protein binding partners of the tetraspanin Sm-TSP-2, a vaccine antigen from the human blood fluke schistosoma mansoni

Abstract

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