Somatic hybrids of higher plants obtained from amiprophosmethyl-resistant mutant Nicotiniana plumbaginifolia

AI Emets*, OP Kundelchuk, AP Smertenko, VG Solodushko, YB Blyum, YY Gleba

*Corresponding author for this work

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Abstract

Interspecific and intertribal somatic hybrids were obtained to study the composition and function of microtubules in hybrid plants. The amiprophosmethyl-resistant mutant Nicotiana plumbaginifolia L. was used as donor; canamycin-resistant mutants N. sylvestris L. and Atropa belladonna served as recipients. Cytogenetic analysis confirmed the hybrid nature of the clones selected. Immunoflourescent analysis showed that constitutions of mitotic spindles in regenerating protoplast, isolated from the hybrid NpAb-107 and the mutant N. plunbaginifolia, show no change after a 2-hour treatment with 5 mu M of amiprophosmethyl; in A. belladonna, the division spindle is completely destroyed under these conditions. Tubulin was isolated from the hybrid NpAb-107 and separated by two-dimensional electrophoresis. The results showed that NpAb-107 has the beta-tubulin isoform specific for N. plumbaginifolia in addition to all isoforms of A. belladonna.

Original languageUndefined/Unknown
Pages (from-to)1104-1111
Number of pages8
JournalGENETIKA
Volume32
Issue number8
Publication statusPublished - Aug 1996

Keywords

  • FUSION
  • PROTOPLASTS
  • NICOTIANA-PLUMBAGINIFOLIA
  • PROTEINS
  • CHLOROPLAST SEGREGATION

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