TY - JOUR
T1 - Specific Monoclonal Antibody-Based Enzyme Immunoassay for Sensitive and Reliable Detection of Alternaria Mycotoxin Iso-Tenuazonic Acid in Food Products
AU - Xiao, Zhi-Li
AU - Wang, Ya-Li
AU - Shen, Yu-Dong
AU - Xu, Zhen-Lin
AU - Dong, Jie-Xian
AU - Wang, Hong
AU - Situ, Chen
AU - Wang, Feng
AU - Yang, Jin-Yi
AU - Lei, Hong-Tao
AU - Sun, Yuan-Ming
PY - 2017/9/14
Y1 - 2017/9/14
N2 - In this paper, we report the development of a sensitiveand specific monoclonal antibody-based immunodiagnosticmethod for the detection of iso-tenuazonic acid (ITeA),an Alternaria mycotoxin, in food samples. The ITeA wasderivatized with hydrazine hydrate to produce the antigen(E)-3-(1-hydrazonoethyl)-4-hydroxy-5-isobutyl-1H-pyrrol-2(5H)-one (ITeAH) which was further reacted with glyoxalicacid to generate the hapten (E)-2-((Z)-(1-(4-hydroxy-5-isobutyl-2-oxo-2,5-dihydro-1H-pyrrol-3-yl)ethylidene)(ITeAHGA) which was used as an immunogen after conjugationto bovine serum albumin (BSA). A highly specific monoclonalantibody selectively binding to ITeAH was generatedvia the hybridoma technique and subsequently used to constructa heterologous indirect competitive enzyme-linked immunosorbentassay (icELISA) using ITeAH as the competitiveantigen for the detection of ITeA with a limit of detection(LOD) of 0.5 ng/mL. Under the optimum conditions, the developedicELISA is highly sensitive (IC50 = 7.8 ng/mL) withrecovery rates ranged from 82.3 to 109.8% for spiked foodsamples. The comparative analysis of results revealed a goodcorrelation between the icELISA and the standard HPLC-MS/MS method, confirming the suitability of the developedicELISA for screening and detection of mycotoxin ITeA infood samples.
AB - In this paper, we report the development of a sensitiveand specific monoclonal antibody-based immunodiagnosticmethod for the detection of iso-tenuazonic acid (ITeA),an Alternaria mycotoxin, in food samples. The ITeA wasderivatized with hydrazine hydrate to produce the antigen(E)-3-(1-hydrazonoethyl)-4-hydroxy-5-isobutyl-1H-pyrrol-2(5H)-one (ITeAH) which was further reacted with glyoxalicacid to generate the hapten (E)-2-((Z)-(1-(4-hydroxy-5-isobutyl-2-oxo-2,5-dihydro-1H-pyrrol-3-yl)ethylidene)(ITeAHGA) which was used as an immunogen after conjugationto bovine serum albumin (BSA). A highly specific monoclonalantibody selectively binding to ITeAH was generatedvia the hybridoma technique and subsequently used to constructa heterologous indirect competitive enzyme-linked immunosorbentassay (icELISA) using ITeAH as the competitiveantigen for the detection of ITeA with a limit of detection(LOD) of 0.5 ng/mL. Under the optimum conditions, the developedicELISA is highly sensitive (IC50 = 7.8 ng/mL) withrecovery rates ranged from 82.3 to 109.8% for spiked foodsamples. The comparative analysis of results revealed a goodcorrelation between the icELISA and the standard HPLC-MS/MS method, confirming the suitability of the developedicELISA for screening and detection of mycotoxin ITeA infood samples.
U2 - 10.1007/s12161-017-1033-9
DO - 10.1007/s12161-017-1033-9
M3 - Article
SN - 1936-9751
VL - 11
SP - 635
EP - 645
JO - Food Analytical Methods
JF - Food Analytical Methods
IS - 3
ER -