Speckled ZnO nanograss electrochemical sensor for staphylococcus epidermidis detection

Agnes Purwidyantri; Ching Hsiang Chen; Liang Yih Chen; Chien Chung Chen; Ji Dung Luo; Chiuan Chian Chiou; Ya Chung Tian; Chan Yu Lin; Chia Ming Yang; Hsin Chih Lai; Chao Sung Lai

doi:10.1149/2.0811706jes

Speckled ZnO nanograss electrochemical sensor for staphylococcus epidermidis detection

Agnes Purwidyantri
, Ching Hsiang Chen
, Liang Yih Chen
, Chien Chung Chen
, Ji Dung Luo
, Chiuan Chian Chiou
, Ya Chung Tian
, Chan Yu Lin
, Chia Ming Yang
, Hsin Chih Lai
, Chao Sung Lai

Research output: Contribution to journal › Article › peer-review

33 Citations (Scopus)

Abstract

Zinc oxide nanograss (ZnONG) decorated with gold (Au) nanospeckles is demonstrated as a sensing platform for bacterial DNA hybridization in this study. Thermal evaporation of 5 nm Au on hydrothermally synthesized ZnONG generates highly dense speckles, which contributes to an approximately two-fold enhancement of the effective surface area and diffusion coefficient in cyclic voltammetry (CV) measurement compared to the non-speckled nanograss. Selective capture of molecular probes from our predesigned sequence of S. epidermidis 16S rRNA onto nanospeckled ZnONG indicated a specific interaction with the DNA target from this prevalently isolated skin pathogen through hybridization screening. The fabricated DNA biosensor demonstrated great ability to quantify a wide range of complementary DNA target, from 10 pM to 1 μM. Moreover, it attained an impressive limit of detection (LOD) of 0.506 pM, as determined by the charge-transfer resistance (R_CT), upon DNA hybridization in an electrochemical impedance spectroscopy (EIS) analysis. The proposed structure of the hybrid Au-speckled ZnONG effectively improved the electrocatalytic properties and electron migration of electrochemical DNA detection due to the increment of the surface area, which consequently renders this structure a potential platform for array-based DNA sensor development.

Original language	English
Pages (from-to)	B205
Journal	Journal of the Electrochemical Society
Volume	164
Issue number	6
DOIs	https://doi.org/10.1149/2.0811706jes
Publication status	Published - 01 Apr 2017
Externally published	Yes

Bibliographical note

Funding Information:
The authors wholeheartedly thank the Ministry of Science and Technology (MOST) of the Republic of China under contract no. MOST 105-2632-E-182-001 and MOST 105-2221-E-182-066 and the Chang Gung Memorial Hospital (CGMH) under project no. CMRPD1C0031, CMRPD2A0091, CMRPD2D0071, CM-RPD2D0072, CMRPD2F0021, CMRPD3D0111, CMRPD3D0112, and CMRPD2D0051.

Publisher Copyright:
© 2017 The Electrochemical Society.

ASJC Scopus subject areas

Electronic, Optical and Magnetic Materials
Renewable Energy, Sustainability and the Environment
Surfaces, Coatings and Films
Electrochemistry
Materials Chemistry

UN SDGs

This output contributes to the following UN Sustainable Development Goals (SDGs)

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10.1149/2.0811706jesLicence: Unspecified

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title = "Speckled ZnO nanograss electrochemical sensor for staphylococcus epidermidis detection",

abstract = "Zinc oxide nanograss (ZnONG) decorated with gold (Au) nanospeckles is demonstrated as a sensing platform for bacterial DNA hybridization in this study. Thermal evaporation of 5 nm Au on hydrothermally synthesized ZnONG generates highly dense speckles, which contributes to an approximately two-fold enhancement of the effective surface area and diffusion coefficient in cyclic voltammetry (CV) measurement compared to the non-speckled nanograss. Selective capture of molecular probes from our predesigned sequence of S. epidermidis 16S rRNA onto nanospeckled ZnONG indicated a specific interaction with the DNA target from this prevalently isolated skin pathogen through hybridization screening. The fabricated DNA biosensor demonstrated great ability to quantify a wide range of complementary DNA target, from 10 pM to 1 μM. Moreover, it attained an impressive limit of detection (LOD) of 0.506 pM, as determined by the charge-transfer resistance (RCT), upon DNA hybridization in an electrochemical impedance spectroscopy (EIS) analysis. The proposed structure of the hybrid Au-speckled ZnONG effectively improved the electrocatalytic properties and electron migration of electrochemical DNA detection due to the increment of the surface area, which consequently renders this structure a potential platform for array-based DNA sensor development.",

author = "Agnes Purwidyantri and Chen, \{Ching Hsiang\} and Chen, \{Liang Yih\} and Chen, \{Chien Chung\} and Luo, \{Ji Dung\} and Chiou, \{Chiuan Chian\} and Tian, \{Ya Chung\} and Lin, \{Chan Yu\} and Yang, \{Chia Ming\} and Lai, \{Hsin Chih\} and Lai, \{Chao Sung\}",

note = "Funding Information: The authors wholeheartedly thank the Ministry of Science and Technology (MOST) of the Republic of China under contract no. MOST 105-2632-E-182-001 and MOST 105-2221-E-182-066 and the Chang Gung Memorial Hospital (CGMH) under project no. CMRPD1C0031, CMRPD2A0091, CMRPD2D0071, CM-RPD2D0072, CMRPD2F0021, CMRPD3D0111, CMRPD3D0112, and CMRPD2D0051. Publisher Copyright: {\textcopyright} 2017 The Electrochemical Society.",

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T1 - Speckled ZnO nanograss electrochemical sensor for staphylococcus epidermidis detection

AU - Purwidyantri, Agnes

AU - Chen, Ching Hsiang

AU - Chen, Liang Yih

AU - Chen, Chien Chung

AU - Luo, Ji Dung

AU - Chiou, Chiuan Chian

AU - Tian, Ya Chung

AU - Lin, Chan Yu

AU - Yang, Chia Ming

AU - Lai, Hsin Chih

AU - Lai, Chao Sung

N1 - Funding Information: The authors wholeheartedly thank the Ministry of Science and Technology (MOST) of the Republic of China under contract no. MOST 105-2632-E-182-001 and MOST 105-2221-E-182-066 and the Chang Gung Memorial Hospital (CGMH) under project no. CMRPD1C0031, CMRPD2A0091, CMRPD2D0071, CM-RPD2D0072, CMRPD2F0021, CMRPD3D0111, CMRPD3D0112, and CMRPD2D0051. Publisher Copyright: © 2017 The Electrochemical Society.

PY - 2017/4/1

Y1 - 2017/4/1

N2 - Zinc oxide nanograss (ZnONG) decorated with gold (Au) nanospeckles is demonstrated as a sensing platform for bacterial DNA hybridization in this study. Thermal evaporation of 5 nm Au on hydrothermally synthesized ZnONG generates highly dense speckles, which contributes to an approximately two-fold enhancement of the effective surface area and diffusion coefficient in cyclic voltammetry (CV) measurement compared to the non-speckled nanograss. Selective capture of molecular probes from our predesigned sequence of S. epidermidis 16S rRNA onto nanospeckled ZnONG indicated a specific interaction with the DNA target from this prevalently isolated skin pathogen through hybridization screening. The fabricated DNA biosensor demonstrated great ability to quantify a wide range of complementary DNA target, from 10 pM to 1 μM. Moreover, it attained an impressive limit of detection (LOD) of 0.506 pM, as determined by the charge-transfer resistance (RCT), upon DNA hybridization in an electrochemical impedance spectroscopy (EIS) analysis. The proposed structure of the hybrid Au-speckled ZnONG effectively improved the electrocatalytic properties and electron migration of electrochemical DNA detection due to the increment of the surface area, which consequently renders this structure a potential platform for array-based DNA sensor development.

AB - Zinc oxide nanograss (ZnONG) decorated with gold (Au) nanospeckles is demonstrated as a sensing platform for bacterial DNA hybridization in this study. Thermal evaporation of 5 nm Au on hydrothermally synthesized ZnONG generates highly dense speckles, which contributes to an approximately two-fold enhancement of the effective surface area and diffusion coefficient in cyclic voltammetry (CV) measurement compared to the non-speckled nanograss. Selective capture of molecular probes from our predesigned sequence of S. epidermidis 16S rRNA onto nanospeckled ZnONG indicated a specific interaction with the DNA target from this prevalently isolated skin pathogen through hybridization screening. The fabricated DNA biosensor demonstrated great ability to quantify a wide range of complementary DNA target, from 10 pM to 1 μM. Moreover, it attained an impressive limit of detection (LOD) of 0.506 pM, as determined by the charge-transfer resistance (RCT), upon DNA hybridization in an electrochemical impedance spectroscopy (EIS) analysis. The proposed structure of the hybrid Au-speckled ZnONG effectively improved the electrocatalytic properties and electron migration of electrochemical DNA detection due to the increment of the surface area, which consequently renders this structure a potential platform for array-based DNA sensor development.

U2 - 10.1149/2.0811706jes

DO - 10.1149/2.0811706jes

M3 - Article

AN - SCOPUS:85035100037

SN - 0013-4651

VL - 164

SP - B205

JO - Journal of the Electrochemical Society

JF - Journal of the Electrochemical Society

IS - 6

ER -

Speckled ZnO nanograss electrochemical sensor for staphylococcus epidermidis detection

Abstract

Bibliographical note

ASJC Scopus subject areas

UN SDGs

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