SPR immunosensor for the detection of okadaic acid in mussels using magnetic particles as antibody carriers

D. Garibo, K. Campbell, A. Casanova, P. de la Iglesia, M. Fernández-Tejedor, J. Diogène, Christopher Elliott, M. Campàs

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29 Citations (Scopus)

Abstract

Protein G-coated magnetic particles (MPs) were used as immobilisation supports for an antibody against okadaic acid (MAb(OA)) and carriers into a surface plasmon resonance (SPR) device for the development of a direct competitive immunosensor for okadaic acid (OA). SPR analysis of MAb(OA)-MP conjugates demonstrated that conjugations were successful with complete immobilisation of all the antibody biomolecules onto the MPs. Moreover, MAb(OA)-MP conjugates provided up to 11-fold higher SPR signals, compared to free MAb(OA). The use of conjugates in the direct competition assay provided a 3-fold lower LOD mu g/L (2.6 mu g of OA/L, equivalent to 12 mu g of OA/kg mussel meat). The presence of mussel matrix did not interfere in the OA quantification as seen in the calibration curves. Mussel samples, obtained from Ebro Delta's bays (NW Mediterranean) during a diarrheic shellfish poisoning (DSP) event and in the presence of Dinophysis sacculus, an OA producer, in the shellfish production area, were analysed with the MP-based SPR immunosensor. The OA contents correlated with those obtained by liquid chromatography-tandem mass spectrometry (LC-MS/MS) (y = 0.984x -5.273, R-2 = 0.789, p <0.001) and by mouse bioassay (MBA).
Original languageEnglish
Pages (from-to)822-828
Number of pages7
JournalSensors and Actuators B: Chemical
Volume190
Early online date25 Sep 2013
DOIs
Publication statusPublished - Jan 2014

Keywords

  • Okadaic acid (OA)
  • Monoclonal antibody (MAb)
  • Protein G-coated magnetic particles (MPs)
  • Surface plasmon resonance (SPR)
  • Liquid chromatography-tandem mass spectrometry (LC-MS/MS)
  • Mussel
  • SURFACE-PLASMON RESONANCE
  • PARALYTIC SHELLFISH
  • IMMUNOAFFINITY EXTRACTION
  • POISONING TOXINS
  • DOMOIC ACID
  • BIOSENSOR
  • NANOPARTICLES
  • FOOD

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