Stable isotope labeling by amino acids in cell culture (SILAC) - an introduction for biologists

Robert L.J. Graham, Michael J. Sweredoski, Sonja Hess*

*Corresponding author for this work

Research output: Contribution to journalArticlepeer-review

5 Citations (Scopus)

Abstract

Since its introduction in 2002 'stable isotope labeling by amino acids in cell culture' (SILAC) has become a major tool for quantitative proteomics. Stable isotopes are incorporated into proteins by introduction of labeled amino acids in growth medium. This methodology's compatibility with virtually all cell culture conditions, ease of implementation into existing workflows and the high quality quantitative data that can be obtained have made it the quantitative method of choice for many laboratories. Although originally used for mammalian cell cultures, it has been adapted to a number of organisms including yeast, bacteria, plants and higher organisms. This review will look at the use of SILAC as a key tool in quantitative biology. The purpose of this review is to furnish the reader with a basic understanding of the fundamental principles of SILAC including its implementation, application, bioinformatic tools for its analyses and potential problems that one should be mindful of.

Original languageEnglish
Pages (from-to)2-16
Number of pages15
JournalCurrent Proteomics
Volume8
Issue number1
DOIs
Publication statusPublished - 23 Mar 2011
Externally publishedYes

Keywords

  • Mass spectrometry
  • Proteomics
  • SILAC
  • Stable isotope labeling by amino acids in cell culture

ASJC Scopus subject areas

  • Biochemistry
  • Molecular Biology

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