Stoichiometric Molecularly Imprinted Polymers for the Recognition of Anti-Cancer Pro-drug Tegafur

Paula Mattos dos Santos, Andrew J. Hall, Panagiotis Manesiotis

Research output: Contribution to journalArticle

9 Citations (Scopus)
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Abstract

Molecularly Imprinted Polymers (MIPs) targeting tegafur, an anti-cancer 5-fluorouracil pro-drug, have been prepared by stoichiometric imprinting using 2,6-bis(acrylamido)pyridine (BAAPy) as the functional monomer. Solution association between tegafur and BAAPy was studied by 1H NMR titration, which confirmed the formation of 1:1 complexes with an affinity constant of 574±15 M-1 ¬in CDCl3. Evaluation of the synthesised materials by HPLC and equilibrium rebinding experiments revealed high selectivity of the imprinted polymer for the pro-drug versus 5-fluorouracil and other competing analytes, with maximum imprinting factors of 25.3 and a binding capacity of 45.1 μmol g-1. The synthesised imprinted polymer was employed in solid-phase extraction of the pro-drug using an optimised protocol that included a simple wash with the porogen used in the preparation of the material. Tegafur recoveries of up to 96% were achieved from aqueous samples and 92% from urine samples spiked with the template and three competing analytes. The results demonstrate the potential of the prepared polymers in the pre-concentration of tegafur from biological samples, which could be an invaluable tool in the monitoring of patient compliance and drug uptake and excretion.
Original languageEnglish
Pages (from-to)197-203
JournalJournal of Chromatography B: Analytical Technologies in the Biomedical and Life Sciences
Volume1021
Early online date09 Dec 2015
DOIs
Publication statusPublished - 15 May 2016

Keywords

  • tegafur
  • 5-fluorouracil
  • bioanalysis
  • molecularly imprinted polymers
  • solid-phase extraction

ASJC Scopus subject areas

  • Analytical Chemistry
  • Materials Chemistry

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