Activities per year
Abstract
Molecularly Imprinted Polymers (MIPs) targeting tegafur, an anti-cancer 5-fluorouracil pro-drug, have been prepared by stoichiometric imprinting using 2,6-bis(acrylamido)pyridine (BAAPy) as the functional monomer. Solution association between tegafur and BAAPy was studied by 1H NMR titration, which confirmed the formation of 1:1 complexes with an affinity constant of 574±15 M-1 ¬in CDCl3. Evaluation of the synthesised materials by HPLC and equilibrium rebinding experiments revealed high selectivity of the imprinted polymer for the pro-drug versus 5-fluorouracil and other competing analytes, with maximum imprinting factors of 25.3 and a binding capacity of 45.1 μmol g-1. The synthesised imprinted polymer was employed in solid-phase extraction of the pro-drug using an optimised protocol that included a simple wash with the porogen used in the preparation of the material. Tegafur recoveries of up to 96% were achieved from aqueous samples and 92% from urine samples spiked with the template and three competing analytes. The results demonstrate the potential of the prepared polymers in the pre-concentration of tegafur from biological samples, which could be an invaluable tool in the monitoring of patient compliance and drug uptake and excretion.
Original language | English |
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Pages (from-to) | 197-203 |
Journal | Journal of Chromatography B: Analytical Technologies in the Biomedical and Life Sciences |
Volume | 1021 |
Early online date | 09 Dec 2015 |
DOIs | |
Publication status | Published - 15 May 2016 |
Keywords
- tegafur
- 5-fluorouracil
- bioanalysis
- molecularly imprinted polymers
- solid-phase extraction
ASJC Scopus subject areas
- Analytical Chemistry
- Materials Chemistry
Fingerprint
Dive into the research topics of 'Stoichiometric Molecularly Imprinted Polymers for the Recognition of Anti-Cancer Pro-drug Tegafur'. Together they form a unique fingerprint.Activities
- 1 Editorial activity
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Journal of Chromatography B: Analytical Technologies in the Biomedical and Life Sciences (Journal)
Manesiotis, P. (Guest editor)
2016 → …Activity: Publication peer-review and editorial work types › Editorial activity
Research output
- 15 Citations
- 1 Editorial
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Affinity-based separations in bioanalysis
Manesiotis, P. & Theodoridis, G., 15 May 2016, In: Journal of Chromatography B: Analytical Technologies in the Biomedical and Life Sciences. 1021, p. 1-2 2 p.Research output: Contribution to journal › Editorial › peer-review