Structural basis of hypoxic gene regulation by the Rv0081 transcription factor of Mycobacterium tuberculosis.

Ashwani  Kumar; Swastik  Phulera; Arshad Rizvi; Parshuram J.  Sonawane; Hemendra S.  Panwar; Sharmistha   Banerjee; Arvind  Sahu; Shekhar C.  Mande

doi:10.1002/1873-3468.13375

Structural basis of hypoxic gene regulation by the Rv0081 transcription factor of Mycobacterium tuberculosis.

Ashwani Kumar , Swastik Phulera, Arshad Rizvi, Parshuram J. Sonawane, Hemendra S. Panwar, Sharmistha Banerjee, Arvind Sahu , Shekhar C. Mande

Research output: Contribution to journal › Article › peer-review

8 Citations (Scopus)

Abstract

The transcription factor Rv0081 of Mycobacterium tuberculosis controls hypoxic gene expression and acts as a regulatory hub in the latent phase of tuberculosis (TB) infection. We report here the crystal structure of Rv0081 at 2.9 Å resolution revealing that it belongs to the well-known ArsR/SmtB family proteins. However, unlike other members in this family, Rv0081 has neither a metal-binding domain nor does it possess Cys residues, suggesting an alternate mechanism of gene regulation. Our structural and biochemical analyses suggest the molecular basis for the recognition of self-regulatory DNA sequences and a plausible mechanism of regulation of Rv0081 in the latent phase of TB infection. DATABASE: Structural data are available in the Protein Data Bank under the accession number - 6JMI.

Original language	English
Journal	FEBS Letters
Volume	593
Issue number	9
Early online date	28 Mar 2019
DOIs	https://doi.org/10.1002/1873-3468.13375
Publication status	Published - 26 Apr 2019
Externally published	Yes

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title = "Structural basis of hypoxic gene regulation by the Rv0081 transcription factor of Mycobacterium tuberculosis.",

abstract = "The transcription factor Rv0081 of Mycobacterium tuberculosis controls hypoxic gene expression and acts as a regulatory hub in the latent phase of tuberculosis (TB) infection. We report here the crystal structure of Rv0081 at 2.9 {\AA} resolution revealing that it belongs to the well-known ArsR/SmtB family proteins. However, unlike other members in this family, Rv0081 has neither a metal-binding domain nor does it possess Cys residues, suggesting an alternate mechanism of gene regulation. Our structural and biochemical analyses suggest the molecular basis for the recognition of self-regulatory DNA sequences and a plausible mechanism of regulation of Rv0081 in the latent phase of TB infection. DATABASE: Structural data are available in the Protein Data Bank under the accession number - 6JMI.",

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AU - Kumar , Ashwani

AU - Phulera, Swastik

AU - Rizvi, Arshad

AU - Sonawane, Parshuram J.

AU - Panwar, Hemendra S.

AU - Banerjee, Sharmistha

AU - Sahu , Arvind

AU - Mande, Shekhar C.

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Y1 - 2019/4/26

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AB - The transcription factor Rv0081 of Mycobacterium tuberculosis controls hypoxic gene expression and acts as a regulatory hub in the latent phase of tuberculosis (TB) infection. We report here the crystal structure of Rv0081 at 2.9 Å resolution revealing that it belongs to the well-known ArsR/SmtB family proteins. However, unlike other members in this family, Rv0081 has neither a metal-binding domain nor does it possess Cys residues, suggesting an alternate mechanism of gene regulation. Our structural and biochemical analyses suggest the molecular basis for the recognition of self-regulatory DNA sequences and a plausible mechanism of regulation of Rv0081 in the latent phase of TB infection. DATABASE: Structural data are available in the Protein Data Bank under the accession number - 6JMI.

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Structural basis of hypoxic gene regulation by the Rv0081 transcription factor of Mycobacterium tuberculosis.

Abstract

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