Abstract
Racemic (1R*,2R*)-1,2-dihydroxy-[1- 13C 1]propylphosphonic acid and 1-hydroxy-[1- 13C 1]acetone were synthesized and fed to R. huakuii PMY1. Alanine and a mixture of valine and methionine were isolated as their N-acetyl derivatives from the cell hydrolysate by reversed-phase HPLC and analyzed by NMR spectroscopy. It was found that the carbon atoms of the respective carboxyl groups were highly 13C-labeled (up to 65 %). Hydroxyacetone is therefore considered an obligatory intermediate of the biodegradation of fosfomycin by R. huakuii PMY1.
Original language | English |
---|---|
Pages (from-to) | 13341-13348 |
Number of pages | 8 |
Journal | Chemistry - A European Journal |
Volume | 17 |
Issue number | 47 |
Early online date | 20 Oct 2011 |
DOIs | |
Publication status | Published - 18 Nov 2011 |
ASJC Scopus subject areas
- General Chemistry