Study of aflatoxin exposure in a Brazilian population using an aflatoxin - albumin biomarker

V. M. Scussel; P. Haas; Y. Y. Gong; C. P. Turner; C. P. Wild

Study of aflatoxin exposure in a Brazilian population using an aflatoxin - albumin biomarker

V. M. Scussel, P. Haas, Y. Y. Gong, C. P. Turner, C. P. Wild

Research output: Chapter in Book/Report/Conference proceeding › Conference contribution

Abstract

The evaluation of exposure to aflatoxins (AF) by measurement of the level of contamination in food is hampered due to the heterogeneous distribution of AF in food. Therefore, an alternative is to estimate the exposure using specific biological markers (biomarkers) based on an understanding of the metabolism of the compound. For AF, these include aflatoxin-N-7-guanine in the urine, or AFB(1)-albumin (AF-alb) in the blood. This study assessed the level of exposure to AF in Brazilian individuals using a biomarker approach, i.e. the AF-alb adducts. Blood samples were collected from urban residents (n=50; aged 18-52) in June 1999, at the Blood Center of Antonio Carlos de Camargo Hospital, Sao Paulo, Brazil. AF-alb adduct levels were determined, by ELISA following serum albumin extraction and digestion. AF-alb adducts were detected in 31/50 (62%) samples [range 0-57.3 pg AFB(1)-lys adducts/mg of blood albumin (pg/mg)]. The mean level of positives was 14.9 pg/mg and males had the two highest levels measured (57.1 and 57.3 pg/mg). There was no correlation with age or profession. This is the first study of Brazilian, or indeed South American, individuals that has determined exposure to AF at the individual level using a biomarker approach. These levels are similar to those observed in the Philippines. These data warrant further investigation of both the sources and consequences of exposure to this potent toxin in Brazil.

Original language	English
Title of host publication	MYCOTOXINS AND PHYCOTOXINS, PROCEEDINGS
Editors	H Njapau, S Trujillo, HP VanEgmond, DL Park
Place of Publication	WAGENINGEN
Publisher	Wageningen Academic Publishers
Pages	197-202
Number of pages	6
ISBN (Print)	978-90-8686-007-4
Publication status	Published - 2006
Event	11th International IUPAC Symposium on Mycotoxins and Phycotoxins - Bethesda, United States Duration: 17 May 2004 → 21 May 2004

Conference

Conference	11th International IUPAC Symposium on Mycotoxins and Phycotoxins
Country/Territory	United States
City	Bethesda
Period	17/05/2004 → 21/05/2004

Cite this

@inproceedings{59da93e48c834c8aaefaa767a396b5fa,

title = "Study of aflatoxin exposure in a Brazilian population using an aflatoxin - albumin biomarker",

abstract = "The evaluation of exposure to aflatoxins (AF) by measurement of the level of contamination in food is hampered due to the heterogeneous distribution of AF in food. Therefore, an alternative is to estimate the exposure using specific biological markers (biomarkers) based on an understanding of the metabolism of the compound. For AF, these include aflatoxin-N-7-guanine in the urine, or AFB(1)-albumin (AF-alb) in the blood. This study assessed the level of exposure to AF in Brazilian individuals using a biomarker approach, i.e. the AF-alb adducts. Blood samples were collected from urban residents (n=50; aged 18-52) in June 1999, at the Blood Center of Antonio Carlos de Camargo Hospital, Sao Paulo, Brazil. AF-alb adduct levels were determined, by ELISA following serum albumin extraction and digestion. AF-alb adducts were detected in 31/50 (62%) samples [range 0-57.3 pg AFB(1)-lys adducts/mg of blood albumin (pg/mg)]. The mean level of positives was 14.9 pg/mg and males had the two highest levels measured (57.1 and 57.3 pg/mg). There was no correlation with age or profession. This is the first study of Brazilian, or indeed South American, individuals that has determined exposure to AF at the individual level using a biomarker approach. These levels are similar to those observed in the Philippines. These data warrant further investigation of both the sources and consequences of exposure to this potent toxin in Brazil.",

author = "Scussel, {V. M.} and P. Haas and Gong, {Y. Y.} and Turner, {C. P.} and Wild, {C. P.}",

year = "2006",

language = "English",

isbn = "978-90-8686-007-4",

pages = "197--202",

editor = "H Njapau and S Trujillo and HP VanEgmond and DL Park",

booktitle = "MYCOTOXINS AND PHYCOTOXINS, PROCEEDINGS",

publisher = "Wageningen Academic Publishers",

note = "11th International IUPAC Symposium on Mycotoxins and Phycotoxins ; Conference date: 17-05-2004 Through 21-05-2004",

}

Scussel, VM, Haas, P, Gong, YY, Turner, CP & Wild, CP 2006, Study of aflatoxin exposure in a Brazilian population using an aflatoxin - albumin biomarker. in H Njapau, S Trujillo, HP VanEgmond & DL Park (eds), MYCOTOXINS AND PHYCOTOXINS, PROCEEDINGS. Wageningen Academic Publishers, WAGENINGEN, pp. 197-202, 11th International IUPAC Symposium on Mycotoxins and Phycotoxins, Bethesda, United States, 17/05/2004.

Study of aflatoxin exposure in a Brazilian population using an aflatoxin - albumin biomarker. / Scussel, V. M.; Haas, P.; Gong, Y. Y. et al.
MYCOTOXINS AND PHYCOTOXINS, PROCEEDINGS. ed. / H Njapau; S Trujillo; HP VanEgmond; DL Park. WAGENINGEN: Wageningen Academic Publishers, 2006. p. 197-202.

Research output: Chapter in Book/Report/Conference proceeding › Conference contribution

TY - GEN

T1 - Study of aflatoxin exposure in a Brazilian population using an aflatoxin - albumin biomarker

AU - Scussel, V. M.

AU - Haas, P.

AU - Gong, Y. Y.

AU - Turner, C. P.

AU - Wild, C. P.

PY - 2006

Y1 - 2006

N2 - The evaluation of exposure to aflatoxins (AF) by measurement of the level of contamination in food is hampered due to the heterogeneous distribution of AF in food. Therefore, an alternative is to estimate the exposure using specific biological markers (biomarkers) based on an understanding of the metabolism of the compound. For AF, these include aflatoxin-N-7-guanine in the urine, or AFB(1)-albumin (AF-alb) in the blood. This study assessed the level of exposure to AF in Brazilian individuals using a biomarker approach, i.e. the AF-alb adducts. Blood samples were collected from urban residents (n=50; aged 18-52) in June 1999, at the Blood Center of Antonio Carlos de Camargo Hospital, Sao Paulo, Brazil. AF-alb adduct levels were determined, by ELISA following serum albumin extraction and digestion. AF-alb adducts were detected in 31/50 (62%) samples [range 0-57.3 pg AFB(1)-lys adducts/mg of blood albumin (pg/mg)]. The mean level of positives was 14.9 pg/mg and males had the two highest levels measured (57.1 and 57.3 pg/mg). There was no correlation with age or profession. This is the first study of Brazilian, or indeed South American, individuals that has determined exposure to AF at the individual level using a biomarker approach. These levels are similar to those observed in the Philippines. These data warrant further investigation of both the sources and consequences of exposure to this potent toxin in Brazil.

AB - The evaluation of exposure to aflatoxins (AF) by measurement of the level of contamination in food is hampered due to the heterogeneous distribution of AF in food. Therefore, an alternative is to estimate the exposure using specific biological markers (biomarkers) based on an understanding of the metabolism of the compound. For AF, these include aflatoxin-N-7-guanine in the urine, or AFB(1)-albumin (AF-alb) in the blood. This study assessed the level of exposure to AF in Brazilian individuals using a biomarker approach, i.e. the AF-alb adducts. Blood samples were collected from urban residents (n=50; aged 18-52) in June 1999, at the Blood Center of Antonio Carlos de Camargo Hospital, Sao Paulo, Brazil. AF-alb adduct levels were determined, by ELISA following serum albumin extraction and digestion. AF-alb adducts were detected in 31/50 (62%) samples [range 0-57.3 pg AFB(1)-lys adducts/mg of blood albumin (pg/mg)]. The mean level of positives was 14.9 pg/mg and males had the two highest levels measured (57.1 and 57.3 pg/mg). There was no correlation with age or profession. This is the first study of Brazilian, or indeed South American, individuals that has determined exposure to AF at the individual level using a biomarker approach. These levels are similar to those observed in the Philippines. These data warrant further investigation of both the sources and consequences of exposure to this potent toxin in Brazil.

M3 - Conference contribution

SN - 978-90-8686-007-4

SP - 197

EP - 202

BT - MYCOTOXINS AND PHYCOTOXINS, PROCEEDINGS

A2 - Njapau, H

A2 - Trujillo, S

A2 - VanEgmond, HP

A2 - Park, DL

PB - Wageningen Academic Publishers

CY - WAGENINGEN

T2 - 11th International IUPAC Symposium on Mycotoxins and Phycotoxins

Y2 - 17 May 2004 through 21 May 2004

ER -

Study of aflatoxin exposure in a Brazilian population using an aflatoxin - albumin biomarker

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