Substrate evaluation for a microbeam endstation using unstained cell imaging.

G. Flaccavento, M. Folkard, J.A. Noble, Kevin Prise, B. Vojnovic

Research output: Contribution to journalArticle

2 Citations (Scopus)

Abstract

A cellular imaging system, optimized for unstained cells seeded onto a thin substrate, is under development. This system will be a component of the endstation for the microbeam cell-irradiation facility at the University of Surrey. Previous irradiation experiments at the Gray Cancer Institute (GCI) have used Mylar film to support the cells [Folkard, M., Prise, K., Schettino, G., Shao, C., Gilchrist, S., Vojnovic, B., 2005. New insights into the cellular response to radiation using microbeams. Nucl. Instrum. Methods B 231, 189-194]. Although suitable for fluorescence microscopy, the Mylar often creates excessive optical noise when used with non-fluorescent microscopy. A variety of substrates are being investigated to provide appropriate optical clarity, cell adhesion, and radiation attenuation. This paper reports on our investigations to date.
Original languageEnglish
Pages (from-to)460-463
Number of pages4
JournalApplied radiation and isotopes : including data, instrumentation and methods for use in agriculture, industry and medicine
Volume67(3)
Issue number3
DOIs
Publication statusPublished - Mar 2009

ASJC Scopus subject areas

  • Radiation

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