TY - JOUR
T1 - Substrate evaluation for a microbeam endstation using unstained cell imaging.
AU - Flaccavento, G.
AU - Folkard, M.
AU - Noble, J.A.
AU - Prise, Kevin
AU - Vojnovic, B.
PY - 2009/3
Y1 - 2009/3
N2 - A cellular imaging system, optimized for unstained cells seeded onto a thin substrate, is under development. This system will be a component of the endstation for the microbeam cell-irradiation facility at the University of Surrey. Previous irradiation experiments at the Gray Cancer Institute (GCI) have used Mylar film to support the cells [Folkard, M., Prise, K., Schettino, G., Shao, C., Gilchrist, S., Vojnovic, B., 2005. New insights into the cellular response to radiation using microbeams. Nucl. Instrum. Methods B 231, 189-194]. Although suitable for fluorescence microscopy, the Mylar often creates excessive optical noise when used with non-fluorescent microscopy. A variety of substrates are being investigated to provide appropriate optical clarity, cell adhesion, and radiation attenuation. This paper reports on our investigations to date.
AB - A cellular imaging system, optimized for unstained cells seeded onto a thin substrate, is under development. This system will be a component of the endstation for the microbeam cell-irradiation facility at the University of Surrey. Previous irradiation experiments at the Gray Cancer Institute (GCI) have used Mylar film to support the cells [Folkard, M., Prise, K., Schettino, G., Shao, C., Gilchrist, S., Vojnovic, B., 2005. New insights into the cellular response to radiation using microbeams. Nucl. Instrum. Methods B 231, 189-194]. Although suitable for fluorescence microscopy, the Mylar often creates excessive optical noise when used with non-fluorescent microscopy. A variety of substrates are being investigated to provide appropriate optical clarity, cell adhesion, and radiation attenuation. This paper reports on our investigations to date.
UR - http://www.scopus.com/inward/record.url?scp=58549093733&partnerID=8YFLogxK
U2 - 10.1016/j.apradiso.2008.06.017
DO - 10.1016/j.apradiso.2008.06.017
M3 - Article
C2 - 18684631
VL - 67(3)
SP - 460
EP - 463
JO - Applied radiation and isotopes : including data, instrumentation and methods for use in agriculture, industry and medicine
JF - Applied radiation and isotopes : including data, instrumentation and methods for use in agriculture, industry and medicine
SN - 0969-8043
IS - 3
ER -
