A cellular imaging system, optimized for unstained cells seeded onto a thin substrate, is under development. This system will be a component of the endstation for the microbeam cell-irradiation facility at the University of Surrey. Previous irradiation experiments at the Gray Cancer Institute (GCI) have used Mylar film to support the cells [Folkard, M., Prise, K., Schettino, G., Shao, C., Gilchrist, S., Vojnovic, B., 2005. New insights into the cellular response to radiation using microbeams. Nucl. Instrum. Methods B 231, 189-194]. Although suitable for fluorescence microscopy, the Mylar often creates excessive optical noise when used with non-fluorescent microscopy. A variety of substrates are being investigated to provide appropriate optical clarity, cell adhesion, and radiation attenuation. This paper reports on our investigations to date.
|Number of pages||4|
|Journal||Applied radiation and isotopes : including data, instrumentation and methods for use in agriculture, industry and medicine|
|Publication status||Published - Mar 2009|
ASJC Scopus subject areas
Flaccavento, G., Folkard, M., Noble, J. A., Prise, K., & Vojnovic, B. (2009). Substrate evaluation for a microbeam endstation using unstained cell imaging. Applied radiation and isotopes : including data, instrumentation and methods for use in agriculture, industry and medicine, 67(3)(3), 460-463. https://doi.org/10.1016/j.apradiso.2008.06.017