Successful elimination of Pseudomonas aeruginosa biofilms with a novel bacteriophage

Pseudomonas aeruginosa is a prevalent opportunistic pathogen and is a leading cause of nosocomial infections around the world. Its ability to form biofilms increases its tolerance to antibiotics and with the number of multidrug resistant isolates on the rise, treatment becomes a complex puzzle as the choice of antibiotics available is diminished. The use of bacteriophages show a great potential as an alternative, novel therapy for eradicating these isolates. Two phages, 휑NFS and RusC, were isolated, and amplified from phage cocktails and were tested for activity against biofilms of P. aeruginosa PAO1 using an MBEC assay. Both phages showed excellent activity against the strain with RusC completely eradicating the biofilm at a phage titre of 107 plaque forming units/mL.