Supramolecular ionic liquid gels for enzyme entrapment

Hasan T. Imam; Kyle Hill; Andrew Reid; Stefan Mix; Patricia C. Marr; Andrew C. Marr

doi:10.1021/acssuschemeng.3c00517

Supramolecular ionic liquid gels for enzyme entrapment

Hasan T. Imam, Kyle Hill, Andrew Reid, Stefan Mix, Patricia C. Marr^*, Andrew C. Marr^*

^*Corresponding author for this work

School of Chemistry and Chemical Engineering

Research output: Contribution to journal › Article › peer-review

12 Citations (Scopus)

66 Downloads (Pure)

Abstract

Reported herein is an entrapment method for enzyme immobilization that does not require the formation of new covalent bonds. Ionic liquid supramolecular gels are formed containing enzymes that can be shaped into gel beads and act as recyclable immobilized biocatalysts. The gel was formed from two components, a hydrophobic phosphonium ionic liquid and a low molecular weight gelator derived from the amino acid phenylalanine. Gel-entrapped lipase from Aneurinibacillus thermoaerophilus was recycled for 10 runs over 3 days without loss of activity and retained activity for at least 150 days. The procedure does not form covalent bonds upon gel formation, which is supramolecular, and no bonds are formed between the enzyme and the solid support.

Original language	English
Pages (from-to)	6829–6837
Number of pages	9
Journal	ACS Sustainable Chemistry & Engineering
Volume	11
Issue number	18
Early online date	24 Apr 2023
DOIs	https://doi.org/10.1021/acssuschemeng.3c00517
Publication status	Published - 08 May 2023

Keywords

Enzyme entrapment
Ionic liquid
Ionic liquid gel
Letter
Low molecular weight gelators
Supramolecular gel

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10.1021/acssuschemeng.3c00517Licence: CC BY

Supramolecular ionic liquid gels for enzyme entrapment
Copyright 2023 The Authors. This is an open access article published under a Creative Commons Attribution License (https://creativecommons.org/licenses/by/4.0/), which permits unrestricted use, distribution and reproduction in any medium, provided the author and source are cited.
Final published version, 3.04 MBLicence: CC BY

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title = "Supramolecular ionic liquid gels for enzyme entrapment",

abstract = "Reported herein is an entrapment method for enzyme immobilization that does not require the formation of new covalent bonds. Ionic liquid supramolecular gels are formed containing enzymes that can be shaped into gel beads and act as recyclable immobilized biocatalysts. The gel was formed from two components, a hydrophobic phosphonium ionic liquid and a low molecular weight gelator derived from the amino acid phenylalanine. Gel-entrapped lipase from Aneurinibacillus thermoaerophilus was recycled for 10 runs over 3 days without loss of activity and retained activity for at least 150 days. The procedure does not form covalent bonds upon gel formation, which is supramolecular, and no bonds are formed between the enzyme and the solid support.",

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doi = "10.1021/acssuschemeng.3c00517",

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T1 - Supramolecular ionic liquid gels for enzyme entrapment

AU - Imam, Hasan T.

AU - Hill, Kyle

AU - Reid, Andrew

AU - Mix, Stefan

AU - Marr, Patricia C.

AU - Marr, Andrew C.

PY - 2023/5/8

Y1 - 2023/5/8

N2 - Reported herein is an entrapment method for enzyme immobilization that does not require the formation of new covalent bonds. Ionic liquid supramolecular gels are formed containing enzymes that can be shaped into gel beads and act as recyclable immobilized biocatalysts. The gel was formed from two components, a hydrophobic phosphonium ionic liquid and a low molecular weight gelator derived from the amino acid phenylalanine. Gel-entrapped lipase from Aneurinibacillus thermoaerophilus was recycled for 10 runs over 3 days without loss of activity and retained activity for at least 150 days. The procedure does not form covalent bonds upon gel formation, which is supramolecular, and no bonds are formed between the enzyme and the solid support.

AB - Reported herein is an entrapment method for enzyme immobilization that does not require the formation of new covalent bonds. Ionic liquid supramolecular gels are formed containing enzymes that can be shaped into gel beads and act as recyclable immobilized biocatalysts. The gel was formed from two components, a hydrophobic phosphonium ionic liquid and a low molecular weight gelator derived from the amino acid phenylalanine. Gel-entrapped lipase from Aneurinibacillus thermoaerophilus was recycled for 10 runs over 3 days without loss of activity and retained activity for at least 150 days. The procedure does not form covalent bonds upon gel formation, which is supramolecular, and no bonds are formed between the enzyme and the solid support.

KW - Enzyme entrapment

KW - Ionic liquid

KW - Ionic liquid gel

KW - Letter

KW - Low molecular weight gelators

KW - Supramolecular gel

U2 - 10.1021/acssuschemeng.3c00517

DO - 10.1021/acssuschemeng.3c00517

M3 - Article

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SP - 6829

EP - 6837

JO - ACS Sustainable Chemistry & Engineering

JF - ACS Sustainable Chemistry & Engineering

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ER -

Supramolecular ionic liquid gels for enzyme entrapment

Abstract

Keywords

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