This paper reports a surface modification of epoxy-based negative photoresist SU-8 for reducing its autofluorescence while enhancing its biofunctionality. By covalently depositing a thin layer of 20 nm Au nanoparticles (AuNPs) onto the SU-8 surface, we found that the AuNPs-coated SU-8 surface is much less fluorescent than the untreated SU-8. Moreover, DNA probes can easily be immobilized on the Au surface and are thermally stable over a wide range of temperature. These improvements will benefit bioanalytical applications such as DNA hybridization and solid-phase PCR (SP-PCR).
|Number of pages||3|
|Publication status||Published - 2011|
|Event||Fifteenth International Conference on Miniaturized Systems for Chemistry and Life Sciences (TAS 2011) - Seattle, United States|
Duration: 02 Oct 2011 → 06 Oct 2011
|Conference||Fifteenth International Conference on Miniaturized Systems for Chemistry and Life Sciences (TAS 2011)|
|Period||02/10/2011 → 06/10/2011|
Cao, C., Birtwell, SW., Hogberg, J., Wolff, A., Morgan, H., & Bang, DD. (2011). Surface Modification of Photoresist SU-8 for Low Autofluorescence and Bioanalytical Applications. 1161-1163. Paper presented at Fifteenth International Conference on Miniaturized Systems for Chemistry and Life Sciences (TAS 2011), Seattle, United States. http://www.rsc.org/images/LOC/2011/PDFs/Papers/389_0159.pdf