Surface plasmon resonance biosensor for the determination of 3-methyl-quinoxaline-2-carboxylic acid, the marker residue of olaquindox, in swine tissues

Dapeng Peng, Owen Kavanagh, Haijiao Gao, Xiya Zhang, Sijun Deng, Dongmei Chen, Zhenli Liu, Changqing Xie, Zonghui Yuan, Chen Situ

Research output: Contribution to journalArticle

Abstract

To monitor the illegal use of olaquindox in animals, a monoclonal antibody-based surface plasmon resonance (SPR) biosensor method has been developed to detect 3-methyl-quinoxaline-2-carboxylic acid, the marker residues of olaquindox, in swine tissues. The limit of detection was 1.4 µg kg-1 in swine muscle and 2.7 µg kg-1 in swine liver, which are lower than the EU recommended concentration (10 µg kg-1). The recoveries were from 82% to 104.6%, with coefficients of variation of less than 12.2%. Good correlations between SPR and HPLC results (r = 0.9806, muscle; r = 0.9698, liver) and between SPR and ic-ELISA results (r = 0.9918, muscle; r = 0.9873, liver) were observed in the affected tissues, which demonstrated the reliability of the SPR method. This method would be a rapid and reliable tool for the screening of the residues of olaquindox in the edible tissues of animals.
Original languageEnglish
JournalFood Chemistry
Early online dateApr 2019
DOIs
Publication statusEarly online date - Apr 2019

Fingerprint

olaquindox
quinoxalines
surface plasmon resonance
Surface Plasmon Resonance
biosensors
Surface plasmon resonance
Biosensing Techniques
carboxylic acids
Biosensors
Swine
Liver
Tissue
Muscle
swine
Muscles
muscles
liver
Animals
animal tissues
Limit of Detection

Cite this

Peng, Dapeng ; Kavanagh, Owen ; Gao, Haijiao ; Zhang, Xiya ; Deng, Sijun ; Chen, Dongmei ; Liu, Zhenli ; Xie, Changqing ; Yuan, Zonghui ; Situ, Chen. / Surface plasmon resonance biosensor for the determination of 3-methyl-quinoxaline-2-carboxylic acid, the marker residue of olaquindox, in swine tissues. In: Food Chemistry. 2019.
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abstract = "To monitor the illegal use of olaquindox in animals, a monoclonal antibody-based surface plasmon resonance (SPR) biosensor method has been developed to detect 3-methyl-quinoxaline-2-carboxylic acid, the marker residues of olaquindox, in swine tissues. The limit of detection was 1.4 µg kg-1 in swine muscle and 2.7 µg kg-1 in swine liver, which are lower than the EU recommended concentration (10 µg kg-1). The recoveries were from 82{\%} to 104.6{\%}, with coefficients of variation of less than 12.2{\%}. Good correlations between SPR and HPLC results (r = 0.9806, muscle; r = 0.9698, liver) and between SPR and ic-ELISA results (r = 0.9918, muscle; r = 0.9873, liver) were observed in the affected tissues, which demonstrated the reliability of the SPR method. This method would be a rapid and reliable tool for the screening of the residues of olaquindox in the edible tissues of animals.",
author = "Dapeng Peng and Owen Kavanagh and Haijiao Gao and Xiya Zhang and Sijun Deng and Dongmei Chen and Zhenli Liu and Changqing Xie and Zonghui Yuan and Chen Situ",
year = "2019",
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doi = "10.1016/j.foodchem.2019.04.022",
language = "English",
journal = "Food Chemistry",
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Surface plasmon resonance biosensor for the determination of 3-methyl-quinoxaline-2-carboxylic acid, the marker residue of olaquindox, in swine tissues. / Peng, Dapeng; Kavanagh, Owen; Gao, Haijiao; Zhang, Xiya; Deng, Sijun; Chen, Dongmei; Liu, Zhenli; Xie, Changqing; Yuan, Zonghui; Situ, Chen.

In: Food Chemistry, 04.2019.

Research output: Contribution to journalArticle

TY - JOUR

T1 - Surface plasmon resonance biosensor for the determination of 3-methyl-quinoxaline-2-carboxylic acid, the marker residue of olaquindox, in swine tissues

AU - Peng, Dapeng

AU - Kavanagh, Owen

AU - Gao, Haijiao

AU - Zhang, Xiya

AU - Deng, Sijun

AU - Chen, Dongmei

AU - Liu, Zhenli

AU - Xie, Changqing

AU - Yuan, Zonghui

AU - Situ, Chen

PY - 2019/4

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N2 - To monitor the illegal use of olaquindox in animals, a monoclonal antibody-based surface plasmon resonance (SPR) biosensor method has been developed to detect 3-methyl-quinoxaline-2-carboxylic acid, the marker residues of olaquindox, in swine tissues. The limit of detection was 1.4 µg kg-1 in swine muscle and 2.7 µg kg-1 in swine liver, which are lower than the EU recommended concentration (10 µg kg-1). The recoveries were from 82% to 104.6%, with coefficients of variation of less than 12.2%. Good correlations between SPR and HPLC results (r = 0.9806, muscle; r = 0.9698, liver) and between SPR and ic-ELISA results (r = 0.9918, muscle; r = 0.9873, liver) were observed in the affected tissues, which demonstrated the reliability of the SPR method. This method would be a rapid and reliable tool for the screening of the residues of olaquindox in the edible tissues of animals.

AB - To monitor the illegal use of olaquindox in animals, a monoclonal antibody-based surface plasmon resonance (SPR) biosensor method has been developed to detect 3-methyl-quinoxaline-2-carboxylic acid, the marker residues of olaquindox, in swine tissues. The limit of detection was 1.4 µg kg-1 in swine muscle and 2.7 µg kg-1 in swine liver, which are lower than the EU recommended concentration (10 µg kg-1). The recoveries were from 82% to 104.6%, with coefficients of variation of less than 12.2%. Good correlations between SPR and HPLC results (r = 0.9806, muscle; r = 0.9698, liver) and between SPR and ic-ELISA results (r = 0.9918, muscle; r = 0.9873, liver) were observed in the affected tissues, which demonstrated the reliability of the SPR method. This method would be a rapid and reliable tool for the screening of the residues of olaquindox in the edible tissues of animals.

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