Surface Plasmon Resonance Biosensor Screening Method for Paralytic Shellfish Poisoning Toxins: A Pilot Interlaboratory Study

H.J. Van Den Top; Christopher Elliott; Simon Haughey; N. Viarino; H.P. Van Egmond; Luis Botana; Katrina Campbell

doi:10.1021/ac2005235

Surface Plasmon Resonance Biosensor Screening Method for Paralytic Shellfish Poisoning Toxins: A Pilot Interlaboratory Study

H.J. Van Den Top, Christopher Elliott, Simon Haughey, N. Viarino, H.P. Van Egmond, Luis Botana, Katrina Campbell

School of Biological Sciences

Research output: Contribution to journal › Article › peer-review

38 Citations (Scopus)

Abstract

A surface plasmon resonance (SPR) optical biosensor method was developed for the detection of paralytic shellfish poisoning (PSP) toxins in shellfish. This application was transferred in the form of a prototype kit to seven laboratories using Biacore QSPR optical biosensor instrumentation for interlaboratory evaluation. Each laboratory received 20 shellfish samples across a range of species including blind duplicates for analysis. The samples consisted of 4 noncontaminated samples spiked in duplicate with a low level of PSP toxins (240 mu g STXcliHCl equivalents/kg), a high level of saxitoxin (825 mu g STXdiHCl/kg), 2 noncontarninated, and 14 naturally contaminated samples. All 7 participating laboratories completed the study, and HorRat values obtained were

Original language	English
Pages (from-to)	4206-4213
Number of pages	8
Journal	Analytical Chemistry
Volume	83
Issue number	11
Early online date	15 Apr 2011
DOIs	https://doi.org/10.1021/ac2005235
Publication status	Published - 01 Jun 2011

ASJC Scopus subject areas

Analytical Chemistry

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10.1021/ac2005235

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title = "Surface Plasmon Resonance Biosensor Screening Method for Paralytic Shellfish Poisoning Toxins: A Pilot Interlaboratory Study",

abstract = "A surface plasmon resonance (SPR) optical biosensor method was developed for the detection of paralytic shellfish poisoning (PSP) toxins in shellfish. This application was transferred in the form of a prototype kit to seven laboratories using Biacore QSPR optical biosensor instrumentation for interlaboratory evaluation. Each laboratory received 20 shellfish samples across a range of species including blind duplicates for analysis. The samples consisted of 4 noncontaminated samples spiked in duplicate with a low level of PSP toxins (240 mu g STXcliHCl equivalents/kg), a high level of saxitoxin (825 mu g STXdiHCl/kg), 2 noncontarninated, and 14 naturally contaminated samples. All 7 participating laboratories completed the study, and HorRat values obtained were",

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Surface Plasmon Resonance Biosensor Screening Method for Paralytic Shellfish Poisoning Toxins: A Pilot Interlaboratory Study. / Van Den Top, H.J.; Elliott, Christopher ; Haughey, Simon; Viarino, N.; Van Egmond, H.P.; Botana, Luis; Campbell, Katrina.

In: Analytical Chemistry, Vol. 83, No. 11, 01.06.2011, p. 4206-4213.

Research output: Contribution to journal › Article › peer-review

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AU - Van Den Top, H.J.

AU - Elliott, Christopher

AU - Haughey, Simon

AU - Viarino, N.

AU - Van Egmond, H.P.

AU - Botana, Luis

AU - Campbell, Katrina

PY - 2011/6/1

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Surface Plasmon Resonance Biosensor Screening Method for Paralytic Shellfish Poisoning Toxins: A Pilot Interlaboratory Study

Abstract

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