Synergistic recognition of an epigenetic DNA element by Pleiohomeotic and a Polycomb core complex

Adone Tielenius Kruythoff-Mohd Sarip, Fabienne Cléard, Rakesh K. Mishra, François Karch, C. Peter Verrijzer*

*Corresponding author for this work

Research output: Contribution to journalArticlepeer-review

74 Citations (Scopus)

Abstract

Polycomb response elements (PREs) are cis-acting DNA elements that mediate epigenetic gene silencing by Polycomb group (PcG) proteins. Here, we report that Pleiohomeotic (PHO) and a multiprotein Polycomb core complex (PCC) bind highly cooperatively to PREs. We identified a conserved sequence motif, named PCC-binding element (PBE), which is required for PcG silencing in vivo. PHO sites and PBEs function as an integrated DNA platform for the synergistic assembly of a repressive PHO/PCC complex. We termed this nucleoprotein complex silenceosome to reflect that the molecular principles underpinning its assemblage are surprisingly similar to those that make an enhanceosome.

Original languageEnglish
Pages (from-to)1755-1760
Number of pages6
JournalGenes and Development
Volume19
Issue number15
DOIs
Publication statusPublished - 01 Aug 2005

Keywords

  • Epigenetic silencing
  • Pleiohomeotic
  • Polycomb
  • PRE
  • Silenceosome

ASJC Scopus subject areas

  • Genetics
  • Developmental Biology

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