Synergistic recognition of an epigenetic DNA element by Pleiohomeotic and a Polycomb core complex

Adone Tielenius Kruythoff-Mohd Sarip; Fabienne Cléard; Rakesh K. Mishra; François Karch; C. Peter Verrijzer

doi:10.1101/gad.347005

Synergistic recognition of an epigenetic DNA element by Pleiohomeotic and a Polycomb core complex

Adone Tielenius Kruythoff-Mohd Sarip, Fabienne Cléard, Rakesh K. Mishra, François Karch, C. Peter Verrijzer^*

^*Corresponding author for this work

School of Medicine, Dentistry and Biomedical Sciences

Research output: Contribution to journal › Article › peer-review

74 Citations (Scopus)

Abstract

Polycomb response elements (PREs) are cis-acting DNA elements that mediate epigenetic gene silencing by Polycomb group (PcG) proteins. Here, we report that Pleiohomeotic (PHO) and a multiprotein Polycomb core complex (PCC) bind highly cooperatively to PREs. We identified a conserved sequence motif, named PCC-binding element (PBE), which is required for PcG silencing in vivo. PHO sites and PBEs function as an integrated DNA platform for the synergistic assembly of a repressive PHO/PCC complex. We termed this nucleoprotein complex silenceosome to reflect that the molecular principles underpinning its assemblage are surprisingly similar to those that make an enhanceosome.

Original language	English
Pages (from-to)	1755-1760
Number of pages	6
Journal	Genes and Development
Volume	19
Issue number	15
DOIs	https://doi.org/10.1101/gad.347005
Publication status	Published - 01 Aug 2005

Keywords

Epigenetic silencing
Pleiohomeotic
Polycomb
PRE
Silenceosome

ASJC Scopus subject areas

Genetics
Developmental Biology

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10.1101/gad.347005

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AU - Cléard, Fabienne

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AU - Karch, François

AU - Verrijzer, C. Peter

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AB - Polycomb response elements (PREs) are cis-acting DNA elements that mediate epigenetic gene silencing by Polycomb group (PcG) proteins. Here, we report that Pleiohomeotic (PHO) and a multiprotein Polycomb core complex (PCC) bind highly cooperatively to PREs. We identified a conserved sequence motif, named PCC-binding element (PBE), which is required for PcG silencing in vivo. PHO sites and PBEs function as an integrated DNA platform for the synergistic assembly of a repressive PHO/PCC complex. We termed this nucleoprotein complex silenceosome to reflect that the molecular principles underpinning its assemblage are surprisingly similar to those that make an enhanceosome.

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