Tailored Microarray Platform for the Detection of Marine Toxins

T.F.H. Bovee, P.J.M. Hendriksen, L. Portier, Silu Wang, Christopher Elliott, H.P. Van Egmond, M.W.F. Nielen, A.A.C.M. Peijnenburg, Ron Hoogenboom

Research output: Contribution to journalArticlepeer-review

23 Citations (Scopus)


Currently, there are no fast in vitro broad spectrum screening bioassays for the detection of marine toxins. The aim of this study was to develop such an assay. In gene expression profiling experiments 17 marker genes were provisionally selected that were differentially regulated in human intestinal Caco-2 cells upon exposure to the lipophilic shellfish poisons azaspiracid-1 (AZA1) or dinophysis toxin-1 (DTX1). These 17 genes together with two control genes were the basis for the design of a tailored microarray platform for the detection of these marine toxins and potentially others. Five out of the 17 selected marker genes on this dedicated DNA microarray gave dear signals, whereby the resulting fingerprints could be used to detect these toxins. CEACAM1, DDIT4, and TUBB3 were up-regulated by both AZA1 and DTX1, TRIB3 was up-regulated by AZA1 only, and OSR2 by DTX1 only. Analysis by singleplex qRT-PCR revealed the up- and down-regulation of the selected RGS16 and NPPB marker genes by DTX1, that were not envisioned by the new developed dedicated array. The qRT-PCR targeting the DDIT4, RSG16 and NPPB genes thus already resulted in a specific pattern for AZA1 and DTX1 indicating that for this specific case qRT-PCR might a be more suitable approach than a dedicated array.
Original languageEnglish
Pages (from-to)8965-8973
Number of pages9
JournalEnvironmental science & technology
Issue number20
Publication statusPublished - 15 Oct 2011

ASJC Scopus subject areas

  • Chemistry(all)
  • Environmental Chemistry


Dive into the research topics of 'Tailored Microarray Platform for the Detection of Marine Toxins'. Together they form a unique fingerprint.

Cite this