Tailored Microarray Platform for the Detection of Marine Toxins

T.F.H. Bovee; P.J.M. Hendriksen; L. Portier; Silu Wang; Christopher Elliott; H.P. Van Egmond; M.W.F. Nielen; A.A.C.M. Peijnenburg; Ron Hoogenboom

doi:10.1021/es2011385

Tailored Microarray Platform for the Detection of Marine Toxins

T.F.H. Bovee, P.J.M. Hendriksen, L. Portier, Silu Wang, Christopher Elliott, H.P. Van Egmond, M.W.F. Nielen, A.A.C.M. Peijnenburg, Ron Hoogenboom

Research output: Contribution to journal › Article › peer-review

26 Citations (Scopus)

Abstract

Currently, there are no fast in vitro broad spectrum screening bioassays for the detection of marine toxins. The aim of this study was to develop such an assay. In gene expression profiling experiments 17 marker genes were provisionally selected that were differentially regulated in human intestinal Caco-2 cells upon exposure to the lipophilic shellfish poisons azaspiracid-1 (AZA1) or dinophysis toxin-1 (DTX1). These 17 genes together with two control genes were the basis for the design of a tailored microarray platform for the detection of these marine toxins and potentially others. Five out of the 17 selected marker genes on this dedicated DNA microarray gave dear signals, whereby the resulting fingerprints could be used to detect these toxins. CEACAM1, DDIT4, and TUBB3 were up-regulated by both AZA1 and DTX1, TRIB3 was up-regulated by AZA1 only, and OSR2 by DTX1 only. Analysis by singleplex qRT-PCR revealed the up- and down-regulation of the selected RGS16 and NPPB marker genes by DTX1, that were not envisioned by the new developed dedicated array. The qRT-PCR targeting the DDIT4, RSG16 and NPPB genes thus already resulted in a specific pattern for AZA1 and DTX1 indicating that for this specific case qRT-PCR might a be more suitable approach than a dedicated array.

Original language	English
Pages (from-to)	8965-8973
Number of pages	9
Journal	Environmental science & technology
Volume	45
Issue number	20
DOIs	https://doi.org/10.1021/es2011385
Publication status	Published - 15 Oct 2011

ASJC Scopus subject areas

General Chemistry
Environmental Chemistry

UN SDGs

This output contributes to the following UN Sustainable Development Goals (SDGs)

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10.1021/es2011385

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title = "Tailored Microarray Platform for the Detection of Marine Toxins",

abstract = "Currently, there are no fast in vitro broad spectrum screening bioassays for the detection of marine toxins. The aim of this study was to develop such an assay. In gene expression profiling experiments 17 marker genes were provisionally selected that were differentially regulated in human intestinal Caco-2 cells upon exposure to the lipophilic shellfish poisons azaspiracid-1 (AZA1) or dinophysis toxin-1 (DTX1). These 17 genes together with two control genes were the basis for the design of a tailored microarray platform for the detection of these marine toxins and potentially others. Five out of the 17 selected marker genes on this dedicated DNA microarray gave dear signals, whereby the resulting fingerprints could be used to detect these toxins. CEACAM1, DDIT4, and TUBB3 were up-regulated by both AZA1 and DTX1, TRIB3 was up-regulated by AZA1 only, and OSR2 by DTX1 only. Analysis by singleplex qRT-PCR revealed the up- and down-regulation of the selected RGS16 and NPPB marker genes by DTX1, that were not envisioned by the new developed dedicated array. The qRT-PCR targeting the DDIT4, RSG16 and NPPB genes thus already resulted in a specific pattern for AZA1 and DTX1 indicating that for this specific case qRT-PCR might a be more suitable approach than a dedicated array.",

author = "T.F.H. Bovee and P.J.M. Hendriksen and L. Portier and Silu Wang and Christopher Elliott and {Van Egmond}, H.P. and M.W.F. Nielen and A.A.C.M. Peijnenburg and Ron Hoogenboom",

year = "2011",

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doi = "10.1021/es2011385",

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AU - Bovee, T.F.H.

AU - Hendriksen, P.J.M.

AU - Portier, L.

AU - Wang, Silu

AU - Elliott, Christopher

AU - Van Egmond, H.P.

AU - Nielen, M.W.F.

AU - Peijnenburg, A.A.C.M.

AU - Hoogenboom, Ron

PY - 2011/10/15

Y1 - 2011/10/15

N2 - Currently, there are no fast in vitro broad spectrum screening bioassays for the detection of marine toxins. The aim of this study was to develop such an assay. In gene expression profiling experiments 17 marker genes were provisionally selected that were differentially regulated in human intestinal Caco-2 cells upon exposure to the lipophilic shellfish poisons azaspiracid-1 (AZA1) or dinophysis toxin-1 (DTX1). These 17 genes together with two control genes were the basis for the design of a tailored microarray platform for the detection of these marine toxins and potentially others. Five out of the 17 selected marker genes on this dedicated DNA microarray gave dear signals, whereby the resulting fingerprints could be used to detect these toxins. CEACAM1, DDIT4, and TUBB3 were up-regulated by both AZA1 and DTX1, TRIB3 was up-regulated by AZA1 only, and OSR2 by DTX1 only. Analysis by singleplex qRT-PCR revealed the up- and down-regulation of the selected RGS16 and NPPB marker genes by DTX1, that were not envisioned by the new developed dedicated array. The qRT-PCR targeting the DDIT4, RSG16 and NPPB genes thus already resulted in a specific pattern for AZA1 and DTX1 indicating that for this specific case qRT-PCR might a be more suitable approach than a dedicated array.

AB - Currently, there are no fast in vitro broad spectrum screening bioassays for the detection of marine toxins. The aim of this study was to develop such an assay. In gene expression profiling experiments 17 marker genes were provisionally selected that were differentially regulated in human intestinal Caco-2 cells upon exposure to the lipophilic shellfish poisons azaspiracid-1 (AZA1) or dinophysis toxin-1 (DTX1). These 17 genes together with two control genes were the basis for the design of a tailored microarray platform for the detection of these marine toxins and potentially others. Five out of the 17 selected marker genes on this dedicated DNA microarray gave dear signals, whereby the resulting fingerprints could be used to detect these toxins. CEACAM1, DDIT4, and TUBB3 were up-regulated by both AZA1 and DTX1, TRIB3 was up-regulated by AZA1 only, and OSR2 by DTX1 only. Analysis by singleplex qRT-PCR revealed the up- and down-regulation of the selected RGS16 and NPPB marker genes by DTX1, that were not envisioned by the new developed dedicated array. The qRT-PCR targeting the DDIT4, RSG16 and NPPB genes thus already resulted in a specific pattern for AZA1 and DTX1 indicating that for this specific case qRT-PCR might a be more suitable approach than a dedicated array.

U2 - 10.1021/es2011385

DO - 10.1021/es2011385

M3 - Article

VL - 45

SP - 8965

EP - 8973

JO - Environmental science & technology

JF - Environmental science & technology

IS - 20

ER -

Tailored Microarray Platform for the Detection of Marine Toxins

Abstract

ASJC Scopus subject areas

UN SDGs

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