Abstract
Noncoding RNA is emerging as an important regulator of gene expression in many organisms. We are characterizing RNA-mediated chromatin silencing of the Arabidopsis major floral repressor gene, FLC. Through suppressor mutagenesis, we identify a requirement for CstF64 and CstF77, two conserved RNA 3'-end-processing factors, in FLC silencing. However, FLC sense transcript 3' processing is not affected in the mutants. Instead, CstF64 and CstF77 are required for 3' processing of FLC antisense transcripts. A specific RNA-binding protein directs their activity to a proximal antisense polyadenylation site. This targeted processing triggers localized histone demethylase activity and results in reduced FLC sense transcription. Targeted 3' processing of antisense transcripts may be a common mechanism triggering transcriptional silencing of the corresponding sense gene.
Original language | English |
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Pages (from-to) | 94-97 |
Number of pages | 4 |
Journal | Science |
Volume | 327 |
Issue number | 5961 |
Early online date | 03 Dec 2009 |
DOIs | |
Publication status | Published - 01 Jan 2010 |
ASJC Scopus subject areas
- General
- Medicine(all)