Taxonomic position, antibiotic resistance and virulence factors of clinical Achromobacter isolates

Ad C. Fluit; Jumamurat R. Bayjanov; María Díez Aguilar; Barry Benaissa-Trouw; Michael M. Tunney; Mireille van Westreenen; Jacques F. Meis; J Stuart Elborn; Rafael Cantón; Miquel B. Ekkelenkamp

doi:10.31083/j.fbs1402009

Taxonomic position, antibiotic resistance and virulence factors of clinical Achromobacter isolates

Ad C. Fluit^*, Jumamurat R. Bayjanov, María Díez Aguilar, Barry Benaissa-Trouw, Michael M. Tunney, Mireille van Westreenen, Jacques F. Meis, J Stuart Elborn, Rafael Cantón, Miquel B. Ekkelenkamp

^*Corresponding author for this work

Research output: Contribution to journal › Article › peer-review

1 Citation (Scopus)

33 Downloads (Pure)

Abstract

The role of Achromobacter species in lung disease remains unclear. The aim of this study was to characterize Achromobacter isolated from persons with cystic fibrosis and from other clinical samples. Whole genome sequences from 101 Achromobacter isolates were determined (81 from patients with cystic fibrosis and 20 from other patients) and analysed. Taxonomic analysis showed nine species including two putative novel species. Thirty-five novel sequence types were present. The most active agent was co-trimoxazole followed by imipenem, but Minimal Inhibitory Concentrations (MICs) were high. Acquired antibiotic resistance genes were rare. Their presence did not correlate with minimal inhibitory concentrations suggesting that other mechanisms are involved. Genes for proposed virulence factors were present in only some isolates. Two putative novel species were identified. The putative virulence properties of Achromobacter involved in infections are variable. Despite the high MICs, acquired resistance genes are uncommon.

Original language	English
Article number	9
Number of pages	12
Journal	Frontiers in bioscience (Scholar edition)
Volume	14
Issue number	2
Early online date	21 Mar 2022
DOIs	https://doi.org/10.31083/j.fbs1402009
Publication status	Published - Jun 2022

Keywords

Gram-Negative Bacterial Infections - drug therapy
Anti-Bacterial Agents - pharmacology - therapeutic use
virulence
Virulence Factors - genetics
antibiotic resistance
Humans
Achromobacter - genetics
cystic fibrosis
taxonomy
Cystic Fibrosis - drug therapy
Drug Resistance, Microbial

Access to Document

10.31083/j.fbs1402009Licence: CC BY

Taxonomic position, antibiotic resistance and virulence factors of clinical Achromobacter isolates
Copyright 2022 the authors. This is an open access article published under a Creative Commons Attribution License (https://creativecommons.org/licenses/by/4.0/), which permits unrestricted use, distribution and reproduction in any medium, provided the author and source are cited.
Final published version, 920 KBLicence: CC BY

Cite this

@article{cd442aa4f11f4b058249a77ed9da8a51,

title = "Taxonomic position, antibiotic resistance and virulence factors of clinical Achromobacter isolates",

abstract = "The role of Achromobacter species in lung disease remains unclear. The aim of this study was to characterize Achromobacter isolated from persons with cystic fibrosis and from other clinical samples. Whole genome sequences from 101 Achromobacter isolates were determined (81 from patients with cystic fibrosis and 20 from other patients) and analysed. Taxonomic analysis showed nine species including two putative novel species. Thirty-five novel sequence types were present. The most active agent was co-trimoxazole followed by imipenem, but Minimal Inhibitory Concentrations (MICs) were high. Acquired antibiotic resistance genes were rare. Their presence did not correlate with minimal inhibitory concentrations suggesting that other mechanisms are involved. Genes for proposed virulence factors were present in only some isolates. Two putative novel species were identified. The putative virulence properties of Achromobacter involved in infections are variable. Despite the high MICs, acquired resistance genes are uncommon.",

keywords = "Gram-Negative Bacterial Infections - drug therapy, Anti-Bacterial Agents - pharmacology - therapeutic use, virulence, Virulence Factors - genetics, antibiotic resistance, Humans, Achromobacter - genetics, cystic fibrosis, taxonomy, Cystic Fibrosis - drug therapy, Drug Resistance, Microbial",

author = "Fluit, {Ad C.} and Bayjanov, {Jumamurat R.} and Aguilar, {Mar{\'i}a D{\'i}ez} and Barry Benaissa-Trouw and Tunney, {Michael M.} and Westreenen, {Mireille van} and Meis, {Jacques F.} and Elborn, {J Stuart} and Rafael Cant{\'o}n and Ekkelenkamp, {Miquel B.}",

year = "2022",

month = jun,

doi = "10.31083/j.fbs1402009",

language = "English",

volume = "14",

journal = "Frontiers in bioscience (Scholar edition)",

issn = "1945-0524",

publisher = "IMR Press Limited",

number = "2",

}

TY - JOUR

T1 - Taxonomic position, antibiotic resistance and virulence factors of clinical Achromobacter isolates

AU - Fluit, Ad C.

AU - Bayjanov, Jumamurat R.

AU - Aguilar, María Díez

AU - Benaissa-Trouw, Barry

AU - Tunney, Michael M.

AU - Westreenen, Mireille van

AU - Meis, Jacques F.

AU - Elborn, J Stuart

AU - Cantón, Rafael

AU - Ekkelenkamp, Miquel B.

PY - 2022/6

Y1 - 2022/6

N2 - The role of Achromobacter species in lung disease remains unclear. The aim of this study was to characterize Achromobacter isolated from persons with cystic fibrosis and from other clinical samples. Whole genome sequences from 101 Achromobacter isolates were determined (81 from patients with cystic fibrosis and 20 from other patients) and analysed. Taxonomic analysis showed nine species including two putative novel species. Thirty-five novel sequence types were present. The most active agent was co-trimoxazole followed by imipenem, but Minimal Inhibitory Concentrations (MICs) were high. Acquired antibiotic resistance genes were rare. Their presence did not correlate with minimal inhibitory concentrations suggesting that other mechanisms are involved. Genes for proposed virulence factors were present in only some isolates. Two putative novel species were identified. The putative virulence properties of Achromobacter involved in infections are variable. Despite the high MICs, acquired resistance genes are uncommon.

AB - The role of Achromobacter species in lung disease remains unclear. The aim of this study was to characterize Achromobacter isolated from persons with cystic fibrosis and from other clinical samples. Whole genome sequences from 101 Achromobacter isolates were determined (81 from patients with cystic fibrosis and 20 from other patients) and analysed. Taxonomic analysis showed nine species including two putative novel species. Thirty-five novel sequence types were present. The most active agent was co-trimoxazole followed by imipenem, but Minimal Inhibitory Concentrations (MICs) were high. Acquired antibiotic resistance genes were rare. Their presence did not correlate with minimal inhibitory concentrations suggesting that other mechanisms are involved. Genes for proposed virulence factors were present in only some isolates. Two putative novel species were identified. The putative virulence properties of Achromobacter involved in infections are variable. Despite the high MICs, acquired resistance genes are uncommon.

KW - Gram-Negative Bacterial Infections - drug therapy

KW - Anti-Bacterial Agents - pharmacology - therapeutic use

KW - virulence

KW - Virulence Factors - genetics

KW - antibiotic resistance

KW - Humans

KW - Achromobacter - genetics

KW - cystic fibrosis

KW - taxonomy

KW - Cystic Fibrosis - drug therapy

KW - Drug Resistance, Microbial

U2 - 10.31083/j.fbs1402009

DO - 10.31083/j.fbs1402009

M3 - Article

C2 - 35730434

SN - 1945-0524

VL - 14

JO - Frontiers in bioscience (Scholar edition)

JF - Frontiers in bioscience (Scholar edition)

IS - 2

M1 - 9

ER -

Taxonomic position, antibiotic resistance and virulence factors of clinical Achromobacter isolates

Abstract

Keywords

Access to Document

Fingerprint

Cite this