TY - JOUR
T1 - Technical note on the exploration of COVID-19 in autopsy material
AU - Humphries, Matthew Phillip
AU - Bingham, Victoria
AU - Abdullah Sidi, Fatima
AU - Craig, Stephanie
AU - Lara, Beatrize
AU - El-Daly, Hesham
AU - O'Doherty, Nicole
AU - Maxwell, Perry
AU - Lewis, Claire
AU - McQuaid, Stephen
AU - Lyness, James
AU - James, Jacqueline
AU - Snead, David R J
AU - Salto-Tellez, Manuel
N1 - © Author(s) (or their employer(s)) 2023. Re-use permitted under CC BY. Published by BMJ.
PY - 2023/1/30
Y1 - 2023/1/30
N2 - Interrogation of immune response in autopsy material from patients with SARS-CoV-2 is potentially significant. We aim to describe a validated protocol for the exploration of the molecular physiopathology of SARS-CoV-2 pulmonary disease using multiplex immunofluorescence (mIF).The application of validated assays for the detection of SARS-CoV-2 in tissues, originally developed in our laboratory in the context of oncology, was used to map the topography and complexity of the adaptive immune response at protein and mRNA levels.SARS-CoV-2 is detectable in situ by protein or mRNA, with a sensitivity that could be in part related to disease stage. In formalin-fixed, paraffin-embedded pneumonia material, multiplex immunofluorescent panels are robust, reliable and quantifiable and can detect topographic variations in inflammation related to pathological processes.Clinical autopsies have relevance in understanding diseases of unknown/complex pathophysiology. In particular, autopsy materials are suitable for the detection of SARS-CoV-2 and for the topographic description of the complex tissue-based immune response using mIF.
AB - Interrogation of immune response in autopsy material from patients with SARS-CoV-2 is potentially significant. We aim to describe a validated protocol for the exploration of the molecular physiopathology of SARS-CoV-2 pulmonary disease using multiplex immunofluorescence (mIF).The application of validated assays for the detection of SARS-CoV-2 in tissues, originally developed in our laboratory in the context of oncology, was used to map the topography and complexity of the adaptive immune response at protein and mRNA levels.SARS-CoV-2 is detectable in situ by protein or mRNA, with a sensitivity that could be in part related to disease stage. In formalin-fixed, paraffin-embedded pneumonia material, multiplex immunofluorescent panels are robust, reliable and quantifiable and can detect topographic variations in inflammation related to pathological processes.Clinical autopsies have relevance in understanding diseases of unknown/complex pathophysiology. In particular, autopsy materials are suitable for the detection of SARS-CoV-2 and for the topographic description of the complex tissue-based immune response using mIF.
U2 - 10.1136/jcp-2022-208525
DO - 10.1136/jcp-2022-208525
M3 - Article
C2 - 36717223
SN - 0021-9746
JO - Journal of Clinical Pathology
JF - Journal of Clinical Pathology
ER -