Technical recommendations for analyzing oxylipins by liquid chromatography-mass spectrometry

Nils Helge Schebb; Nadja Kampschulte; Gerhard Hagn; Kathrin Plitzko; Sven W Meckelmann; Soumita Ghosh; Robin Joshi; Julia Kuligowski; Dajana Vuckovic; Marina T Botana; Ángel Sánchez-Illana; Fereshteh Zandkarimi; Aditi Das; Jun Yang; Louis Schmidt; Antonio Checa; Helen M. Roche; Aaron M Armando; Matthew L Edin; Fred B Lih; Juan J Aristizabal-Henao; Sayuri Miyamoto; Francesca Giuffrida; Arieh Moussaieff; Rosário Domingues; Michael Rothe; Christine Hinz; Ujjalkumar Subhash Das; Katharina M Rund; Ameer Y Taha; Robert K Hofstetter; Markus Werner; Oliver Werz; Astrid S Kahnt; Justine Bertrand-Michel; Pauline Le Faouder; Robert Gurke; Dominique Thomas; Federico Torta; Ivana Milic; Irundika H K Dias; Corinne M Spickett; Denise Biagini; Tommaso Lomonaco; Helena Idborg; Jun-Yan Liu; Maria Fedorova; David A. Ford; Anne Barden; Trevor A Mori; Paul D Kennedy; Kirk Maxey; Julijana Ivanisevic; Hector Gallart-Ayala; Cécile Gladine; Markus Wenk; Jean-Marie Galano; Thierry Durand; Ken D Stark; Coral Barbas; Ulrike Garscha; Stacy L Gelhaus; Uta Ceglarek; Nicolas Flamand; Julian L Griffin; Robert Ahrends; Makoto Arita; Darryl C Zeldin; Francisco J Schopfer; Oswald Quehenberger; Randall Julian; Anna Nicolaou; Ian A Blair; Michael P Murphy; Bruce D Hammock; Bruce Freeman; Gerhard Liebisch; Charles N Serhan; Harald C Köfeler; Per-Johan Jakobsson; Dieter Steinhilber; Michael H Gelb; Michal Holčapek; Ruth Andrew; Martin Giera; Garret A FitzGerald; Robert C Murphy; John W Newman; Edward A Dennis; Kim Ekroos; Ginger L Milne; Miguel A Gijón; Hubert W Vesper; Craig E Wheelock; Valerie B O'Donnell

doi:10.1126/scisignal.adw1245

Technical recommendations for analyzing oxylipins by liquid chromatography-mass spectrometry

Nils Helge Schebb
, Nadja Kampschulte
, Gerhard Hagn
, Kathrin Plitzko
, Sven W Meckelmann
, Soumita Ghosh
, Robin Joshi
, Julia Kuligowski
, Dajana Vuckovic
, Marina T Botana
, Ángel Sánchez-Illana
, Fereshteh Zandkarimi
, Aditi Das
, Jun Yang
, Louis Schmidt
, Antonio Checa
, Helen M. Roche
, Aaron M Armando
, Matthew L Edin
, Fred B Lih

Juan J Aristizabal-Henao, Sayuri Miyamoto, Francesca Giuffrida, Arieh Moussaieff, Rosário Domingues, Michael Rothe, Christine Hinz, Ujjalkumar Subhash Das, Katharina M Rund, Ameer Y Taha, Robert K Hofstetter, Markus Werner, Oliver Werz, Astrid S Kahnt, Justine Bertrand-Michel, Pauline Le Faouder, Robert Gurke, Dominique Thomas, Federico Torta, Ivana Milic, Irundika H K Dias, Corinne M Spickett, Denise Biagini, Tommaso Lomonaco, Helena Idborg, Jun-Yan Liu, Maria Fedorova, David A. Ford, Anne Barden, Trevor A Mori, Paul D Kennedy, Kirk Maxey, Julijana Ivanisevic, Hector Gallart-Ayala, Cécile Gladine, Markus Wenk, Jean-Marie Galano, Thierry Durand, Ken D Stark, Coral Barbas, Ulrike Garscha, Stacy L Gelhaus, Uta Ceglarek, Nicolas Flamand, Julian L Griffin, Robert Ahrends, Makoto Arita, Darryl C Zeldin, Francisco J Schopfer, Oswald Quehenberger, Randall Julian, Anna Nicolaou, Ian A Blair, Michael P Murphy, Bruce D Hammock, Bruce Freeman, Gerhard Liebisch, Charles N Serhan, Harald C Köfeler, Per-Johan Jakobsson, Dieter Steinhilber, Michael H Gelb, Michal Holčapek, Ruth Andrew, Martin Giera, Garret A FitzGerald, Robert C Murphy, John W Newman, Edward A Dennis, Kim Ekroos, Ginger L Milne, Miguel A Gijón, Hubert W Vesper, Craig E Wheelock, Valerie B O'Donnell

School of Biological Sciences

Research output: Contribution to journal › Article › peer-review

41 Citations (Scopus)

Abstract

Several oxylipins are potent lipid mediators that regulate diverse aspects of health and disease and whose quantitative analysis by liquid chromatography-mass spectrometry (LC-MS) presents substantial technical challenges. As members of the lipidomics community, we developed technical recommendations to ensure best practices when quantifying oxylipins by LC-MS.

Original language	English
Article number	eadw1245
Number of pages	3
Journal	Science Signaling
Volume	18
Issue number	887
DOIs	https://doi.org/10.1126/scisignal.adw1245
Publication status	Published - 20 May 2025

Keywords

Mass Spectrometry - methods
Oxylipins - analysis
Liquid Chromatography-Mass Spectrometry
Animals
Chromatography, Liquid - methods
Lipidomics - methods
Humans

Access to Document

10.1126/scisignal.adw1245Licence: Unspecified

Cite this

Schebb, N. H., Kampschulte, N., Hagn, G., Plitzko, K., Meckelmann, S. W., Ghosh, S., Joshi, R., Kuligowski, J., Vuckovic, D., Botana, M. T., Sánchez-Illana, Á., Zandkarimi, F., Das, A., Yang, J., Schmidt, L., Checa, A., Roche, H. M., Armando, A. M., Edin, M. L., ... O'Donnell, V. B. (2025). Technical recommendations for analyzing oxylipins by liquid chromatography-mass spectrometry. Science Signaling, 18(887), Article eadw1245. https://doi.org/10.1126/scisignal.adw1245

@article{99abbdf7f337419b95fc3ccd57bc4b73,

title = "Technical recommendations for analyzing oxylipins by liquid chromatography-mass spectrometry",

abstract = "Several oxylipins are potent lipid mediators that regulate diverse aspects of health and disease and whose quantitative analysis by liquid chromatography-mass spectrometry (LC-MS) presents substantial technical challenges. As members of the lipidomics community, we developed technical recommendations to ensure best practices when quantifying oxylipins by LC-MS. ",

keywords = "Mass Spectrometry - methods, Oxylipins - analysis, Liquid Chromatography-Mass Spectrometry, Animals, Chromatography, Liquid - methods, Lipidomics - methods, Humans",

author = "Schebb, \{Nils Helge\} and Nadja Kampschulte and Gerhard Hagn and Kathrin Plitzko and Meckelmann, \{Sven W\} and Soumita Ghosh and Robin Joshi and Julia Kuligowski and Dajana Vuckovic and Botana, \{Marina T\} and {\'A}ngel S{\'a}nchez-Illana and Fereshteh Zandkarimi and Aditi Das and Jun Yang and Louis Schmidt and Antonio Checa and Roche, \{Helen M.\} and Armando, \{Aaron M\} and Edin, \{Matthew L\} and Lih, \{Fred B\} and Aristizabal-Henao, \{Juan J\} and Sayuri Miyamoto and Francesca Giuffrida and Arieh Moussaieff and Ros{\'a}rio Domingues and Michael Rothe and Christine Hinz and Das, \{Ujjalkumar Subhash\} and Rund, \{Katharina M\} and Taha, \{Ameer Y\} and Hofstetter, \{Robert K\} and Markus Werner and Oliver Werz and Kahnt, \{Astrid S\} and Justine Bertrand-Michel and \{Le Faouder\}, Pauline and Robert Gurke and Dominique Thomas and Federico Torta and Ivana Milic and Dias, \{Irundika H K\} and Spickett, \{Corinne M\} and Denise Biagini and Tommaso Lomonaco and Helena Idborg and Jun-Yan Liu and Maria Fedorova and Ford, \{David A.\} and Anne Barden and Mori, \{Trevor A\} and Kennedy, \{Paul D\} and Kirk Maxey and Julijana Ivanisevic and Hector Gallart-Ayala and C{\'e}cile Gladine and Markus Wenk and Jean-Marie Galano and Thierry Durand and Stark, \{Ken D\} and Coral Barbas and Ulrike Garscha and Gelhaus, \{Stacy L\} and Uta Ceglarek and Nicolas Flamand and Griffin, \{Julian L\} and Robert Ahrends and Makoto Arita and Zeldin, \{Darryl C\} and Schopfer, \{Francisco J\} and Oswald Quehenberger and Randall Julian and Anna Nicolaou and Blair, \{Ian A\} and Murphy, \{Michael P\} and Hammock, \{Bruce D\} and Bruce Freeman and Gerhard Liebisch and Serhan, \{Charles N\} and K{\"o}feler, \{Harald C\} and Per-Johan Jakobsson and Dieter Steinhilber and Gelb, \{Michael H\} and Michal Hol{\v c}apek and Ruth Andrew and Martin Giera and FitzGerald, \{Garret A\} and Murphy, \{Robert C\} and Newman, \{John W\} and Dennis, \{Edward A\} and Kim Ekroos and Milne, \{Ginger L\} and Gij{\'o}n, \{Miguel A\} and Vesper, \{Hubert W\} and Wheelock, \{Craig E\} and O'Donnell, \{Valerie B\}",

year = "2025",

month = may,

day = "20",

doi = "10.1126/scisignal.adw1245",

language = "English",

volume = "18",

journal = "Science Signaling",

issn = "1945-0877",

publisher = "American Association for the Advancement of Science",

number = "887",

}

Schebb, NH, Kampschulte, N, Hagn, G, Plitzko, K, Meckelmann, SW, Ghosh, S, Joshi, R, Kuligowski, J, Vuckovic, D, Botana, MT, Sánchez-Illana, Á, Zandkarimi, F, Das, A, Yang, J, Schmidt, L, Checa, A, Roche, HM, Armando, AM, Edin, ML, Lih, FB, Aristizabal-Henao, JJ, Miyamoto, S, Giuffrida, F, Moussaieff, A, Domingues, R, Rothe, M, Hinz, C, Das, US, Rund, KM, Taha, AY, Hofstetter, RK, Werner, M, Werz, O, Kahnt, AS, Bertrand-Michel, J, Le Faouder, P, Gurke, R, Thomas, D, Torta, F, Milic, I, Dias, IHK, Spickett, CM, Biagini, D, Lomonaco, T, Idborg, H, Liu, J-Y, Fedorova, M, Ford, DA, Barden, A, Mori, TA, Kennedy, PD, Maxey, K, Ivanisevic, J, Gallart-Ayala, H, Gladine, C, Wenk, M, Galano, J-M, Durand, T, Stark, KD, Barbas, C, Garscha, U, Gelhaus, SL, Ceglarek, U, Flamand, N, Griffin, JL, Ahrends, R, Arita, M, Zeldin, DC, Schopfer, FJ, Quehenberger, O, Julian, R, Nicolaou, A, Blair, IA, Murphy, MP, Hammock, BD, Freeman, B, Liebisch, G, Serhan, CN, Köfeler, HC, Jakobsson, P-J, Steinhilber, D, Gelb, MH, Holčapek, M, Andrew, R, Giera, M, FitzGerald, GA, Murphy, RC, Newman, JW, Dennis, EA, Ekroos, K, Milne, GL, Gijón, MA, Vesper, HW, Wheelock, CE & O'Donnell, VB 2025, 'Technical recommendations for analyzing oxylipins by liquid chromatography-mass spectrometry', Science Signaling, vol. 18, no. 887, eadw1245. https://doi.org/10.1126/scisignal.adw1245

TY - JOUR

T1 - Technical recommendations for analyzing oxylipins by liquid chromatography-mass spectrometry

AU - Schebb, Nils Helge

AU - Kampschulte, Nadja

AU - Hagn, Gerhard

AU - Plitzko, Kathrin

AU - Meckelmann, Sven W

AU - Ghosh, Soumita

AU - Joshi, Robin

AU - Kuligowski, Julia

AU - Vuckovic, Dajana

AU - Botana, Marina T

AU - Sánchez-Illana, Ángel

AU - Zandkarimi, Fereshteh

AU - Das, Aditi

AU - Yang, Jun

AU - Schmidt, Louis

AU - Checa, Antonio

AU - Roche, Helen M.

AU - Armando, Aaron M

AU - Edin, Matthew L

AU - Lih, Fred B

AU - Aristizabal-Henao, Juan J

AU - Miyamoto, Sayuri

AU - Giuffrida, Francesca

AU - Moussaieff, Arieh

AU - Domingues, Rosário

AU - Rothe, Michael

AU - Hinz, Christine

AU - Das, Ujjalkumar Subhash

AU - Rund, Katharina M

AU - Taha, Ameer Y

AU - Hofstetter, Robert K

AU - Werner, Markus

AU - Werz, Oliver

AU - Kahnt, Astrid S

AU - Bertrand-Michel, Justine

AU - Le Faouder, Pauline

AU - Gurke, Robert

AU - Thomas, Dominique

AU - Torta, Federico

AU - Milic, Ivana

AU - Dias, Irundika H K

AU - Spickett, Corinne M

AU - Biagini, Denise

AU - Lomonaco, Tommaso

AU - Idborg, Helena

AU - Liu, Jun-Yan

AU - Fedorova, Maria

AU - Ford, David A.

AU - Barden, Anne

AU - Mori, Trevor A

AU - Kennedy, Paul D

AU - Maxey, Kirk

AU - Ivanisevic, Julijana

AU - Gallart-Ayala, Hector

AU - Gladine, Cécile

AU - Wenk, Markus

AU - Galano, Jean-Marie

AU - Durand, Thierry

AU - Stark, Ken D

AU - Barbas, Coral

AU - Garscha, Ulrike

AU - Gelhaus, Stacy L

AU - Ceglarek, Uta

AU - Flamand, Nicolas

AU - Griffin, Julian L

AU - Ahrends, Robert

AU - Arita, Makoto

AU - Zeldin, Darryl C

AU - Schopfer, Francisco J

AU - Quehenberger, Oswald

AU - Julian, Randall

AU - Nicolaou, Anna

AU - Blair, Ian A

AU - Murphy, Michael P

AU - Hammock, Bruce D

AU - Freeman, Bruce

AU - Liebisch, Gerhard

AU - Serhan, Charles N

AU - Köfeler, Harald C

AU - Jakobsson, Per-Johan

AU - Steinhilber, Dieter

AU - Gelb, Michael H

AU - Holčapek, Michal

AU - Andrew, Ruth

AU - Giera, Martin

AU - FitzGerald, Garret A

AU - Murphy, Robert C

AU - Newman, John W

AU - Dennis, Edward A

AU - Ekroos, Kim

AU - Milne, Ginger L

AU - Gijón, Miguel A

AU - Vesper, Hubert W

AU - Wheelock, Craig E

AU - O'Donnell, Valerie B

PY - 2025/5/20

Y1 - 2025/5/20

N2 - Several oxylipins are potent lipid mediators that regulate diverse aspects of health and disease and whose quantitative analysis by liquid chromatography-mass spectrometry (LC-MS) presents substantial technical challenges. As members of the lipidomics community, we developed technical recommendations to ensure best practices when quantifying oxylipins by LC-MS.

AB - Several oxylipins are potent lipid mediators that regulate diverse aspects of health and disease and whose quantitative analysis by liquid chromatography-mass spectrometry (LC-MS) presents substantial technical challenges. As members of the lipidomics community, we developed technical recommendations to ensure best practices when quantifying oxylipins by LC-MS.

KW - Mass Spectrometry - methods

KW - Oxylipins - analysis

KW - Liquid Chromatography-Mass Spectrometry

KW - Animals

KW - Chromatography, Liquid - methods

KW - Lipidomics - methods

KW - Humans

U2 - 10.1126/scisignal.adw1245

DO - 10.1126/scisignal.adw1245

M3 - Article

C2 - 40392938

SN - 1945-0877

VL - 18

JO - Science Signaling

JF - Science Signaling

IS - 887

M1 - eadw1245

ER -

Technical recommendations for analyzing oxylipins by liquid chromatography-mass spectrometry

Abstract

Keywords

Access to Document

Fingerprint

Cite this