Temporal dynamics of the metabolically active rumen bacteria colonizing fresh perennial ryegrass

Sharon A Huws, Joan Elizabeth Edwards, Christopher J Creevey, Pauline Rees Stevens, Wanchang Lin, Susan Elizabeth Girdwood, Justin A Pachebat, Alison H Kingston-Smith

Research output: Contribution to journalArticle

33 Citations (Scopus)

Abstract

This study investigated successional colonization of fresh perennial ryegrass (PRG) by the rumen microbiota over time. Fresh PRG was incubated in sacco in the rumens of three Holstein × Friesian cows over a period of 8 h, with samples recovered at various times. The diversity of attached bacteria was assessed using 454 pyrosequencing of 16S rRNA (cDNA). Results showed that plant epiphytic communities either decreased to low relative abundances or disappeared following rumen incubation, and that temporal colonization of the PRG by the rumen bacteria was biphasic with primary (1 and 2 h) and secondary (4-8 h) events evident with the transition period being with 2-4 h. A decrease in sequence reads pertaining to Succinivibrio spp. and increases in Pseudobutyrivibrio, Roseburia and Ruminococcus spp. (the latter all order Clostridiales) were evident during secondary colonization. Irrespective of temporal changes, the continually high abundances of Butyrivibrio, Fibrobacter, Olsenella and Prevotella suggest that they play a major role in the degradation of the plant. It is clear that a temporal understanding of the functional roles of these microbiota within the rumen is now required to unravel the role of these bacteria in the ruminal degradation of fresh PRG.

Original languageEnglish
JournalFEMS Microbiology Ecology
Volume92
Issue number1
DOIs
Publication statusPublished - Jan 2016

Fingerprint

Lolium
Rumen
colonization
Bacteria
bacterium
Microbiota
functional role
Fibrobacter
Butyrivibrio
Ruminococcus
plant community
Prevotella
relative abundance
incubation
Complementary DNA

Keywords

  • Actinobacteria
  • Animals
  • Bacteria
  • Butyrivibrio
  • Cattle
  • Female
  • Fibrobacter
  • Gastrointestinal Microbiome
  • Lolium
  • Prevotella
  • RNA, Ribosomal, 16S
  • Rumen
  • Ruminococcus
  • Succinivibrionaceae
  • Journal Article
  • Research Support, Non-U.S. Gov't

Cite this

Huws, Sharon A ; Edwards, Joan Elizabeth ; Creevey, Christopher J ; Rees Stevens, Pauline ; Lin, Wanchang ; Girdwood, Susan Elizabeth ; Pachebat, Justin A ; Kingston-Smith, Alison H. / Temporal dynamics of the metabolically active rumen bacteria colonizing fresh perennial ryegrass. In: FEMS Microbiology Ecology. 2016 ; Vol. 92, No. 1.
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abstract = "This study investigated successional colonization of fresh perennial ryegrass (PRG) by the rumen microbiota over time. Fresh PRG was incubated in sacco in the rumens of three Holstein × Friesian cows over a period of 8 h, with samples recovered at various times. The diversity of attached bacteria was assessed using 454 pyrosequencing of 16S rRNA (cDNA). Results showed that plant epiphytic communities either decreased to low relative abundances or disappeared following rumen incubation, and that temporal colonization of the PRG by the rumen bacteria was biphasic with primary (1 and 2 h) and secondary (4-8 h) events evident with the transition period being with 2-4 h. A decrease in sequence reads pertaining to Succinivibrio spp. and increases in Pseudobutyrivibrio, Roseburia and Ruminococcus spp. (the latter all order Clostridiales) were evident during secondary colonization. Irrespective of temporal changes, the continually high abundances of Butyrivibrio, Fibrobacter, Olsenella and Prevotella suggest that they play a major role in the degradation of the plant. It is clear that a temporal understanding of the functional roles of these microbiota within the rumen is now required to unravel the role of these bacteria in the ruminal degradation of fresh PRG.",
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Temporal dynamics of the metabolically active rumen bacteria colonizing fresh perennial ryegrass. / Huws, Sharon A; Edwards, Joan Elizabeth; Creevey, Christopher J; Rees Stevens, Pauline; Lin, Wanchang; Girdwood, Susan Elizabeth; Pachebat, Justin A; Kingston-Smith, Alison H.

In: FEMS Microbiology Ecology, Vol. 92, No. 1, 01.2016.

Research output: Contribution to journalArticle

TY - JOUR

T1 - Temporal dynamics of the metabolically active rumen bacteria colonizing fresh perennial ryegrass

AU - Huws, Sharon A

AU - Edwards, Joan Elizabeth

AU - Creevey, Christopher J

AU - Rees Stevens, Pauline

AU - Lin, Wanchang

AU - Girdwood, Susan Elizabeth

AU - Pachebat, Justin A

AU - Kingston-Smith, Alison H

N1 - © FEMS 2015. All rights reserved. For permissions, please e-mail: journals.permissions@oup.com.

PY - 2016/1

Y1 - 2016/1

N2 - This study investigated successional colonization of fresh perennial ryegrass (PRG) by the rumen microbiota over time. Fresh PRG was incubated in sacco in the rumens of three Holstein × Friesian cows over a period of 8 h, with samples recovered at various times. The diversity of attached bacteria was assessed using 454 pyrosequencing of 16S rRNA (cDNA). Results showed that plant epiphytic communities either decreased to low relative abundances or disappeared following rumen incubation, and that temporal colonization of the PRG by the rumen bacteria was biphasic with primary (1 and 2 h) and secondary (4-8 h) events evident with the transition period being with 2-4 h. A decrease in sequence reads pertaining to Succinivibrio spp. and increases in Pseudobutyrivibrio, Roseburia and Ruminococcus spp. (the latter all order Clostridiales) were evident during secondary colonization. Irrespective of temporal changes, the continually high abundances of Butyrivibrio, Fibrobacter, Olsenella and Prevotella suggest that they play a major role in the degradation of the plant. It is clear that a temporal understanding of the functional roles of these microbiota within the rumen is now required to unravel the role of these bacteria in the ruminal degradation of fresh PRG.

AB - This study investigated successional colonization of fresh perennial ryegrass (PRG) by the rumen microbiota over time. Fresh PRG was incubated in sacco in the rumens of three Holstein × Friesian cows over a period of 8 h, with samples recovered at various times. The diversity of attached bacteria was assessed using 454 pyrosequencing of 16S rRNA (cDNA). Results showed that plant epiphytic communities either decreased to low relative abundances or disappeared following rumen incubation, and that temporal colonization of the PRG by the rumen bacteria was biphasic with primary (1 and 2 h) and secondary (4-8 h) events evident with the transition period being with 2-4 h. A decrease in sequence reads pertaining to Succinivibrio spp. and increases in Pseudobutyrivibrio, Roseburia and Ruminococcus spp. (the latter all order Clostridiales) were evident during secondary colonization. Irrespective of temporal changes, the continually high abundances of Butyrivibrio, Fibrobacter, Olsenella and Prevotella suggest that they play a major role in the degradation of the plant. It is clear that a temporal understanding of the functional roles of these microbiota within the rumen is now required to unravel the role of these bacteria in the ruminal degradation of fresh PRG.

KW - Actinobacteria

KW - Animals

KW - Bacteria

KW - Butyrivibrio

KW - Cattle

KW - Female

KW - Fibrobacter

KW - Gastrointestinal Microbiome

KW - Lolium

KW - Prevotella

KW - RNA, Ribosomal, 16S

KW - Rumen

KW - Ruminococcus

KW - Succinivibrionaceae

KW - Journal Article

KW - Research Support, Non-U.S. Gov't

U2 - 10.1093/femsec/fiv137

DO - 10.1093/femsec/fiv137

M3 - Article

C2 - 26542074

VL - 92

JO - FEMS Microbiology Ecology

JF - FEMS Microbiology Ecology

SN - 0168-6496

IS - 1

ER -