The acute transcriptome response of the midbrain/diencephalon to injury in the adult mummichog (Fundulus heteroclitus)

Eleanor C. Bisese, Chandler M. Ciuba, Amelia L. Davidson, Akanksha Kaushik, Sabrina M. Mullen, Jeremy L. Barth, E. Starr Hazard, Robert C. Wilson, Gary Hardiman, David M. Hollis*

*Corresponding author for this work

Research output: Contribution to journalArticlepeer-review

1 Citation (Scopus)
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Adult fish produce new cells throughout their central nervous system during the course of their lives and maintain a tremendous capacity to repair damaged neural tissue. Much of the focus on understanding brain repair and regeneration in adult fish has been directed at regions of the brainstem and forebrain; however, the mesencephalon (midbrain) and diencephalon have received little attention. We sought to examine differential gene expression in the midbrain/diencephalon in response to injury in the adult fish using RNA-seq. Using the mummichog (Fundulus heteroclitus), we administered a mechanical lesion to the midbrain/diencephalon and examined differentially expressed genes (DEGs) at an acute recovery time of 1 h post-injury. Comparisons of whole transcriptomes derived from isolated RNA of intact and injured midbrain/diencephalic tissue identified 404 DEGs with the vast majority being upregulated. Using qPCR, we validated the upregulation of DEGs pim-2-like, syndecan-4-like, and cd83. Based on genes both familiar and novel regarding the adult brain response to injury, these data provide an extensive molecular profile giving insight into a range of cellular processes involved in the injury response of a brain regenerative-capable vertebrate.

Original languageEnglish
Article number119
Number of pages4
JournalMolecular Brain
Publication statusPublished - 30 Dec 2019


  • Acute
  • Brain injury
  • cd83
  • Diencephalon
  • Midbrain
  • Mummichog
  • pim-2-like
  • syndecan-4-like
  • Transcriptome

ASJC Scopus subject areas

  • Molecular Biology
  • Cellular and Molecular Neuroscience


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