The bioactive effects of casein proteins on enteroendocrine cell health, proliferation and incretin hormone secretion

Anna L Gillespie, Brian D Green

Research output: Contribution to journalArticle

12 Citations (Scopus)

Abstract

Previous studies suggest that casein exerts various anti-diabetic effects. However, it is not known which casein proteins are bioactive, nor their effects on enteroendocrine cells. This study evaluated the effects of intact whole casein, intact individual proteins (alpha, beta and kappa casein) and hydrolysates on an enteroendocrine cell line. High content analysis accurately monitored changes in cell health and intracellular glucagon-like peptide-1 (GLP-1) content. Cheese ripening duration and GLP-1 secretory responses were also considered. Beta casein significantly stimulated enteroendocrine cell proliferation and all caseins were potent GLP-1 secretagogues (except kappa casein). Interestingly the GLP-1 secretory activity was almost always lost or significantly reduced upon hydrolysis with proteolytic enzymes. Only pepsin-derived beta casein hydrolysates had significantly increased potency compared with the intact protein, but this was diminished with prolonged hydrolysis. In conclusion casein proteins are not detrimental to enteroendocrine cells, and alpha and beta casein are particularly beneficial stimulating proliferation and GLP-1 secretion.

Original languageEnglish
Pages (from-to)148-59
Number of pages12
JournalFood Chemistry
Volume211
Early online date22 Apr 2016
DOIs
Publication statusPublished - 15 Nov 2016

Keywords

  • Journal Article

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