The cancer-associated cell migration protein TSPAN1 is under control of androgens and its upregulation increases prostate cancer cell migration

Jennifer Munkley, Urszula L McClurg, Karen E Livermore, Ingrid Ehrmann, Bridget Knight, Paul Mccullagh, John Mcgrath, Malcolm Crundwell, Lorna W Harries, Hing Y Leung, Ian G Mills, Craig N Robson, Prabhakar Rajan, David J Elliott

Research output: Contribution to journalArticlepeer-review

35 Citations (Scopus)
297 Downloads (Pure)

Abstract

Cell migration drives cell invasion and metastatic progression in prostate cancer and is a major cause of mortality and morbidity. However the mechanisms driving cell migration in prostate cancer patients are not fully understood. We previously identified the cancer-associated cell migration protein Tetraspanin 1 (TSPAN1) as a clinically relevant androgen regulated target in prostate cancer. Here we find that TSPAN1 is acutely induced by androgens, and is significantly upregulated in prostate cancer relative to both normal prostate tissue and benign prostate hyperplasia (BPH). We also show for the first time, that TSPAN1 expression in prostate cancer cells controls the expression of key proteins involved in cell migration. Stable upregulation of TSPAN1 in both DU145 and PC3 cells significantly increased cell migration and induced the expression of the mesenchymal markers SLUG and ARF6. Our data suggest TSPAN1 is an androgen-driven contributor to cell survival and motility in prostate cancer.

Original languageEnglish
Article number5249
JournalScientific Reports
Volume7
Issue number1
Early online date12 Jul 2017
DOIs
Publication statusEarly online date - 12 Jul 2017

Keywords

  • Journal Article

Fingerprint

Dive into the research topics of 'The cancer-associated cell migration protein TSPAN1 is under control of androgens and its upregulation increases prostate cancer cell migration'. Together they form a unique fingerprint.

Cite this