The cancer-associated cell migration protein TSPAN1 is under control of androgens and its upregulation increases prostate cancer cell migration

Jennifer Munkley; Urszula L McClurg; Karen E Livermore; Ingrid Ehrmann; Bridget Knight; Paul Mccullagh; John Mcgrath; Malcolm Crundwell; Lorna W Harries; Hing Y Leung; Ian G Mills; Craig N Robson; Prabhakar Rajan; David J Elliott

doi:10.1038/s41598-017-05489-5

The cancer-associated cell migration protein TSPAN1 is under control of androgens and its upregulation increases prostate cancer cell migration

Jennifer Munkley, Urszula L McClurg, Karen E Livermore, Ingrid Ehrmann, Bridget Knight, Paul Mccullagh, John Mcgrath, Malcolm Crundwell, Lorna W Harries, Hing Y Leung, Ian G Mills, Craig N Robson, Prabhakar Rajan, David J Elliott

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Abstract

Cell migration drives cell invasion and metastatic progression in prostate cancer and is a major cause of mortality and morbidity. However the mechanisms driving cell migration in prostate cancer patients are not fully understood. We previously identified the cancer-associated cell migration protein Tetraspanin 1 (TSPAN1) as a clinically relevant androgen regulated target in prostate cancer. Here we find that TSPAN1 is acutely induced by androgens, and is significantly upregulated in prostate cancer relative to both normal prostate tissue and benign prostate hyperplasia (BPH). We also show for the first time, that TSPAN1 expression in prostate cancer cells controls the expression of key proteins involved in cell migration. Stable upregulation of TSPAN1 in both DU145 and PC3 cells significantly increased cell migration and induced the expression of the mesenchymal markers SLUG and ARF6. Our data suggest TSPAN1 is an androgen-driven contributor to cell survival and motility in prostate cancer.

Original language	English
Article number	5249
Journal	Scientific Reports
Volume	7
Issue number	1
Early online date	12 Jul 2017
DOIs	https://doi.org/10.1038/s41598-017-05489-5
Publication status	Early online date - 12 Jul 2017

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Journal Article

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The cancer-associated cell migration protein TSPAN1 is under control of androgens and its upregulation increases prostate cancer cell migration
© The Author(s) 2017This is an open access article published under a Creative Commons Attribution License (https://creativecommons.org/licenses/by/4.0/), which permits unrestricted use, distribution and reproduction in any medium, provided the author and source are cited.
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Munkley, J., McClurg, U. L., Livermore, K. E., Ehrmann, I., Knight, B., Mccullagh, P., Mcgrath, J., Crundwell, M., Harries, L. W., Leung, H. Y., Mills, I. G., Robson, C. N., Rajan, P., & Elliott, D. J. (2017). The cancer-associated cell migration protein TSPAN1 is under control of androgens and its upregulation increases prostate cancer cell migration. Scientific Reports, 7(1), [5249]. https://doi.org/10.1038/s41598-017-05489-5

Munkley, Jennifer ; McClurg, Urszula L ; Livermore, Karen E ; Ehrmann, Ingrid ; Knight, Bridget ; Mccullagh, Paul ; Mcgrath, John ; Crundwell, Malcolm ; Harries, Lorna W ; Leung, Hing Y ; Mills, Ian G ; Robson, Craig N ; Rajan, Prabhakar ; Elliott, David J. / The cancer-associated cell migration protein TSPAN1 is under control of androgens and its upregulation increases prostate cancer cell migration. In: Scientific Reports. 2017 ; Vol. 7, No. 1.

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abstract = "Cell migration drives cell invasion and metastatic progression in prostate cancer and is a major cause of mortality and morbidity. However the mechanisms driving cell migration in prostate cancer patients are not fully understood. We previously identified the cancer-associated cell migration protein Tetraspanin 1 (TSPAN1) as a clinically relevant androgen regulated target in prostate cancer. Here we find that TSPAN1 is acutely induced by androgens, and is significantly upregulated in prostate cancer relative to both normal prostate tissue and benign prostate hyperplasia (BPH). We also show for the first time, that TSPAN1 expression in prostate cancer cells controls the expression of key proteins involved in cell migration. Stable upregulation of TSPAN1 in both DU145 and PC3 cells significantly increased cell migration and induced the expression of the mesenchymal markers SLUG and ARF6. Our data suggest TSPAN1 is an androgen-driven contributor to cell survival and motility in prostate cancer.",

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author = "Jennifer Munkley and McClurg, {Urszula L} and Livermore, {Karen E} and Ingrid Ehrmann and Bridget Knight and Paul Mccullagh and John Mcgrath and Malcolm Crundwell and Harries, {Lorna W} and Leung, {Hing Y} and Mills, {Ian G} and Robson, {Craig N} and Prabhakar Rajan and Elliott, {David J}",

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Munkley, J, McClurg, UL, Livermore, KE, Ehrmann, I, Knight, B, Mccullagh, P, Mcgrath, J, Crundwell, M, Harries, LW, Leung, HY, Mills, IG, Robson, CN, Rajan, P & Elliott, DJ 2017, 'The cancer-associated cell migration protein TSPAN1 is under control of androgens and its upregulation increases prostate cancer cell migration', Scientific Reports, vol. 7, no. 1, 5249. https://doi.org/10.1038/s41598-017-05489-5

The cancer-associated cell migration protein TSPAN1 is under control of androgens and its upregulation increases prostate cancer cell migration. / Munkley, Jennifer; McClurg, Urszula L; Livermore, Karen E; Ehrmann, Ingrid; Knight, Bridget; Mccullagh, Paul; Mcgrath, John; Crundwell, Malcolm; Harries, Lorna W; Leung, Hing Y; Mills, Ian G; Robson, Craig N; Rajan, Prabhakar; Elliott, David J.

In: Scientific Reports, Vol. 7, No. 1, 5249, 12.07.2017.

Research output: Contribution to journal › Article

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AU - Munkley, Jennifer

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AU - Livermore, Karen E

AU - Ehrmann, Ingrid

AU - Knight, Bridget

AU - Mccullagh, Paul

AU - Mcgrath, John

AU - Crundwell, Malcolm

AU - Harries, Lorna W

AU - Leung, Hing Y

AU - Mills, Ian G

AU - Robson, Craig N

AU - Rajan, Prabhakar

AU - Elliott, David J

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AB - Cell migration drives cell invasion and metastatic progression in prostate cancer and is a major cause of mortality and morbidity. However the mechanisms driving cell migration in prostate cancer patients are not fully understood. We previously identified the cancer-associated cell migration protein Tetraspanin 1 (TSPAN1) as a clinically relevant androgen regulated target in prostate cancer. Here we find that TSPAN1 is acutely induced by androgens, and is significantly upregulated in prostate cancer relative to both normal prostate tissue and benign prostate hyperplasia (BPH). We also show for the first time, that TSPAN1 expression in prostate cancer cells controls the expression of key proteins involved in cell migration. Stable upregulation of TSPAN1 in both DU145 and PC3 cells significantly increased cell migration and induced the expression of the mesenchymal markers SLUG and ARF6. Our data suggest TSPAN1 is an androgen-driven contributor to cell survival and motility in prostate cancer.

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