The Caspase-Related Protease Separase (EXTRA SPINDLE POLES) Regulates Cell Polarity and Cytokinesis in Arabidopsis

Panagiotis N. Moschou, Andrei P. Smertenko, Elena A. Minina, Kazutake Fukada, Eugene I. Savenkov, Stephanie Robert, Patrick J. Hussey, Peter V. Bozhkov

Research output: Contribution to specialist publicationArticle

23 Citations (Scopus)

Abstract

Vesicle trafficking plays an important role in cell division, establishment of cell polarity, and translation of environmental cues to developmental responses. However, the molecular mechanisms regulating vesicle trafficking remain poorly understood. Here, we report that the evolutionarily conserved caspase-related protease separase (EXTRA SPINDLE POLES [ESP]) is required for the establishment of cell polarity and cytokinesis in Arabidopsis thaliana. At the cellular level, separase colocalizes with microtubules and RabA2a (for RAS GENES FROM RAT BRAINA2a) GTPase-positive structures. Separase facilitates polar targeting of the auxin efflux carrier PIN-FORMED2 (PIN2) to the rootward side of the root cortex cells. Plants with the radially swollen4 (rsw4) allele with compromised separase activity, in addition to mitotic failure, display isotropic cell growth, perturbation of auxin gradient formation, slower gravitropic response in roots, and cytokinetic failure. Measurements of the dynamics of vesicle markers on the cell plate revealed an overall reduction of the delivery rates of KNOLLE and RabA2a GTPase in separase-deficient roots. Furthermore, dissociation of the clathrin light chain, a protein that plays major role in the formation of coated vesicles, was slower in rsw4 than in the control. Our results demonstrate that separase is a key regulator of vesicle trafficking, which is indispensable for cytokinesis and the establishment of cell polarity.
Original languageEnglish
Pages2171-2186
Number of pages16
Volume25
No.6
Specialist publicationThe Plant Cell
Publication statusPublished - Jun 2013

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separase
caspases
cytokinesis
proteinases
Arabidopsis
guanosinetriphosphatase
auxins
coated vesicles
clathrin
translation (genetics)
microtubules
cell polarity
cell division
cell growth
cortex
Arabidopsis thaliana
cells
alleles

Cite this

Moschou, P. N., Smertenko, A. P., Minina, E. A., Fukada, K., Savenkov, E. I., Robert, S., ... Bozhkov, P. V. (2013). The Caspase-Related Protease Separase (EXTRA SPINDLE POLES) Regulates Cell Polarity and Cytokinesis in Arabidopsis. The Plant Cell, 25(6), 2171-2186.
Moschou, Panagiotis N. ; Smertenko, Andrei P. ; Minina, Elena A. ; Fukada, Kazutake ; Savenkov, Eugene I. ; Robert, Stephanie ; Hussey, Patrick J. ; Bozhkov, Peter V. / The Caspase-Related Protease Separase (EXTRA SPINDLE POLES) Regulates Cell Polarity and Cytokinesis in Arabidopsis. In: The Plant Cell. 2013 ; Vol. 25, No. 6. pp. 2171-2186.
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abstract = "Vesicle trafficking plays an important role in cell division, establishment of cell polarity, and translation of environmental cues to developmental responses. However, the molecular mechanisms regulating vesicle trafficking remain poorly understood. Here, we report that the evolutionarily conserved caspase-related protease separase (EXTRA SPINDLE POLES [ESP]) is required for the establishment of cell polarity and cytokinesis in Arabidopsis thaliana. At the cellular level, separase colocalizes with microtubules and RabA2a (for RAS GENES FROM RAT BRAINA2a) GTPase-positive structures. Separase facilitates polar targeting of the auxin efflux carrier PIN-FORMED2 (PIN2) to the rootward side of the root cortex cells. Plants with the radially swollen4 (rsw4) allele with compromised separase activity, in addition to mitotic failure, display isotropic cell growth, perturbation of auxin gradient formation, slower gravitropic response in roots, and cytokinetic failure. Measurements of the dynamics of vesicle markers on the cell plate revealed an overall reduction of the delivery rates of KNOLLE and RabA2a GTPase in separase-deficient roots. Furthermore, dissociation of the clathrin light chain, a protein that plays major role in the formation of coated vesicles, was slower in rsw4 than in the control. Our results demonstrate that separase is a key regulator of vesicle trafficking, which is indispensable for cytokinesis and the establishment of cell polarity.",
author = "Moschou, {Panagiotis N.} and Smertenko, {Andrei P.} and Minina, {Elena A.} and Kazutake Fukada and Savenkov, {Eugene I.} and Stephanie Robert and Hussey, {Patrick J.} and Bozhkov, {Peter V.}",
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Moschou, PN, Smertenko, AP, Minina, EA, Fukada, K, Savenkov, EI, Robert, S, Hussey, PJ & Bozhkov, PV 2013, 'The Caspase-Related Protease Separase (EXTRA SPINDLE POLES) Regulates Cell Polarity and Cytokinesis in Arabidopsis' The Plant Cell, vol. 25, no. 6, pp. 2171-2186.

The Caspase-Related Protease Separase (EXTRA SPINDLE POLES) Regulates Cell Polarity and Cytokinesis in Arabidopsis. / Moschou, Panagiotis N.; Smertenko, Andrei P.; Minina, Elena A.; Fukada, Kazutake; Savenkov, Eugene I.; Robert, Stephanie ; Hussey, Patrick J. ; Bozhkov, Peter V.

In: The Plant Cell, Vol. 25, No. 6, 06.2013, p. 2171-2186.

Research output: Contribution to specialist publicationArticle

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AU - Moschou, Panagiotis N.

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AU - Minina, Elena A.

AU - Fukada, Kazutake

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AU - Bozhkov, Peter V.

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N2 - Vesicle trafficking plays an important role in cell division, establishment of cell polarity, and translation of environmental cues to developmental responses. However, the molecular mechanisms regulating vesicle trafficking remain poorly understood. Here, we report that the evolutionarily conserved caspase-related protease separase (EXTRA SPINDLE POLES [ESP]) is required for the establishment of cell polarity and cytokinesis in Arabidopsis thaliana. At the cellular level, separase colocalizes with microtubules and RabA2a (for RAS GENES FROM RAT BRAINA2a) GTPase-positive structures. Separase facilitates polar targeting of the auxin efflux carrier PIN-FORMED2 (PIN2) to the rootward side of the root cortex cells. Plants with the radially swollen4 (rsw4) allele with compromised separase activity, in addition to mitotic failure, display isotropic cell growth, perturbation of auxin gradient formation, slower gravitropic response in roots, and cytokinetic failure. Measurements of the dynamics of vesicle markers on the cell plate revealed an overall reduction of the delivery rates of KNOLLE and RabA2a GTPase in separase-deficient roots. Furthermore, dissociation of the clathrin light chain, a protein that plays major role in the formation of coated vesicles, was slower in rsw4 than in the control. Our results demonstrate that separase is a key regulator of vesicle trafficking, which is indispensable for cytokinesis and the establishment of cell polarity.

AB - Vesicle trafficking plays an important role in cell division, establishment of cell polarity, and translation of environmental cues to developmental responses. However, the molecular mechanisms regulating vesicle trafficking remain poorly understood. Here, we report that the evolutionarily conserved caspase-related protease separase (EXTRA SPINDLE POLES [ESP]) is required for the establishment of cell polarity and cytokinesis in Arabidopsis thaliana. At the cellular level, separase colocalizes with microtubules and RabA2a (for RAS GENES FROM RAT BRAINA2a) GTPase-positive structures. Separase facilitates polar targeting of the auxin efflux carrier PIN-FORMED2 (PIN2) to the rootward side of the root cortex cells. Plants with the radially swollen4 (rsw4) allele with compromised separase activity, in addition to mitotic failure, display isotropic cell growth, perturbation of auxin gradient formation, slower gravitropic response in roots, and cytokinetic failure. Measurements of the dynamics of vesicle markers on the cell plate revealed an overall reduction of the delivery rates of KNOLLE and RabA2a GTPase in separase-deficient roots. Furthermore, dissociation of the clathrin light chain, a protein that plays major role in the formation of coated vesicles, was slower in rsw4 than in the control. Our results demonstrate that separase is a key regulator of vesicle trafficking, which is indispensable for cytokinesis and the establishment of cell polarity.

M3 - Article

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SP - 2171

EP - 2186

JO - The Plant Cell

JF - The Plant Cell

SN - 1040-4651

ER -

Moschou PN, Smertenko AP, Minina EA, Fukada K, Savenkov EI, Robert S et al. The Caspase-Related Protease Separase (EXTRA SPINDLE POLES) Regulates Cell Polarity and Cytokinesis in Arabidopsis. The Plant Cell. 2013 Jun;25(6):2171-2186.