The Citrobacter rodentium genome sequence reveals convergent evolution with human pathogenic Escherichia coli

Nicola K. Petty, Richard Bulgin, Valerie F. Crepin, Ana M. Cerdeño-Tárraga, Gunnar N. Schroeder, Michael A. Quail, Nicola Lennard, Craig Corton, Andrew Barron, Louise Clark, Ana L. Toribio, Julian Parkhill, Gordon Dougan, Gad Frankel, Nicholas R. Thomson

Research output: Contribution to journalArticle

100 Citations (Scopus)

Abstract

Citrobacter rodentium (formally Citrobacter freundii biotype 4280) is a highly infectious pathogen that causes colitis and transmissible colonic hyperplasia in mice. In common with enteropathogenic and enterohemorrhagic Escherichia coli (EPEC and EHEC, respectively), C. rodentium exploits a type III secretion system (T3SS) to induce attaching and effacing (A/E) lesions that are essential for virulence. Here, we report the fully annotated genome sequence of the 5.3-Mb chromosome and four plasmids harbored by C. rodentium strain ICC168. The genome sequence revealed key information about the phylogeny of C. rodentium and identified 1,585 C. rodentium-specific (without orthologues in EPEC or EHEC) coding sequences, 10 prophage-like regions, and 17 genomic islands, including the locus for enterocyte effacement (LEE) region, which encodes a T3SS and effector proteins. Among the 29 T3SS effectors found in C. rodentium are all 22 of the core effectors of EPEC strain E2348/69. In addition, we identified a novel C. rodentium effector, named EspS. C. rodentium harbors two type VI secretion systems (T6SS) (CTS1 and CTS2), while EHEC contains only one T6SS (EHS). Our analysis suggests that C. rodentium and EPEC/EHEC have converged on a common host infection strategy through access to a common pool of mobile DNA and that C. rodentium has lost gene functions associated with a previous pathogenic niche.

Original languageEnglish
Pages (from-to)525-538
Number of pages14
JournalJournal of Bacteriology
Volume192
Issue number2
DOIs
Publication statusPublished - Jan 2010
Externally publishedYes

ASJC Scopus subject areas

  • Microbiology
  • Molecular Biology

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