The determination of asialoglycoforms of serum glycoproteins by lectin blotting with Ricinus communis agglutinin

Fionnuala Lundy, G.B. Wisdom

Research output: Contribution to journalArticlepeer-review

8 Citations (Scopus)

Abstract

Serum proteins were fractionated by polyacrylamide gel electrophoresis under denaturing conditions and transferred to nitrocellulose membranes. The blotted polypeptides were probed with biotinylated Ricinus communis lectin (RCA120) followed by streptavidin/alkaline phosphatase. This procedure detected five asialoglycoproteins (a2-macroglobulin, transferrin, a1-antitrypsin, a1-antichymotrypsin and haptoglobin ß chain). The asialoform of the a1-trypsin inhibitor was found to be decreased in inflammation.
Original languageEnglish
Pages (from-to)187-195
Number of pages9
JournalClinica Chimica Acta
Volume205 (3)
DOIs
Publication statusPublished - Feb 1992

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