Abstract
Serum proteins were fractionated by polyacrylamide gel electrophoresis under denaturing conditions and transferred to nitrocellulose membranes. The blotted polypeptides were probed with biotinylated Ricinus communis lectin (RCA120) followed by streptavidin/alkaline phosphatase. This procedure detected five asialoglycoproteins (a2-macroglobulin, transferrin, a1-antitrypsin, a1-antichymotrypsin and haptoglobin ß chain). The asialoform of the a1-trypsin inhibitor was found to be decreased in inflammation.
Original language | English |
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Pages (from-to) | 187-195 |
Number of pages | 9 |
Journal | Clinica Chimica Acta |
Volume | 205 (3) |
DOIs | |
Publication status | Published - Feb 1992 |