The determination of asialoglycoforms of serum glycoproteins by lectin blotting with Ricinus communis agglutinin

Fionnuala Lundy; G.B. Wisdom

doi:10.1016/0009-8981(92)90059-Y

The determination of asialoglycoforms of serum glycoproteins by lectin blotting with Ricinus communis agglutinin

Fionnuala Lundy, G.B. Wisdom

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Abstract

Serum proteins were fractionated by polyacrylamide gel electrophoresis under denaturing conditions and transferred to nitrocellulose membranes. The blotted polypeptides were probed with biotinylated Ricinus communis lectin (RCA120) followed by streptavidin/alkaline phosphatase. This procedure detected five asialoglycoproteins (a2-macroglobulin, transferrin, a1-antitrypsin, a1-antichymotrypsin and haptoglobin ß chain). The asialoform of the a1-trypsin inhibitor was found to be decreased in inflammation.

Original language	English
Pages (from-to)	187-195
Number of pages	9
Journal	Clinica Chimica Acta
Volume	205 (3)
DOIs	https://doi.org/10.1016/0009-8981(92)90059-Y
Publication status	Published - Feb 1992

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title = "The determination of asialoglycoforms of serum glycoproteins by lectin blotting with Ricinus communis agglutinin",

abstract = "Serum proteins were fractionated by polyacrylamide gel electrophoresis under denaturing conditions and transferred to nitrocellulose membranes. The blotted polypeptides were probed with biotinylated Ricinus communis lectin (RCA120) followed by streptavidin/alkaline phosphatase. This procedure detected five asialoglycoproteins (a2-macroglobulin, transferrin, a1-antitrypsin, a1-antichymotrypsin and haptoglobin {\ss} chain). The asialoform of the a1-trypsin inhibitor was found to be decreased in inflammation.",

author = "Fionnuala Lundy and G.B. Wisdom",

year = "1992",

month = feb,

doi = "10.1016/0009-8981(92)90059-Y",

language = "English",

volume = "205 (3)",

pages = "187--195",

journal = "Clinica Chimica Acta",

publisher = "Elsevier B.V.",

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T1 - The determination of asialoglycoforms of serum glycoproteins by lectin blotting with Ricinus communis agglutinin

AU - Lundy, Fionnuala

AU - Wisdom, G.B.

PY - 1992/2

Y1 - 1992/2

N2 - Serum proteins were fractionated by polyacrylamide gel electrophoresis under denaturing conditions and transferred to nitrocellulose membranes. The blotted polypeptides were probed with biotinylated Ricinus communis lectin (RCA120) followed by streptavidin/alkaline phosphatase. This procedure detected five asialoglycoproteins (a2-macroglobulin, transferrin, a1-antitrypsin, a1-antichymotrypsin and haptoglobin ß chain). The asialoform of the a1-trypsin inhibitor was found to be decreased in inflammation.

AB - Serum proteins were fractionated by polyacrylamide gel electrophoresis under denaturing conditions and transferred to nitrocellulose membranes. The blotted polypeptides were probed with biotinylated Ricinus communis lectin (RCA120) followed by streptavidin/alkaline phosphatase. This procedure detected five asialoglycoproteins (a2-macroglobulin, transferrin, a1-antitrypsin, a1-antichymotrypsin and haptoglobin ß chain). The asialoform of the a1-trypsin inhibitor was found to be decreased in inflammation.

U2 - 10.1016/0009-8981(92)90059-Y

DO - 10.1016/0009-8981(92)90059-Y

M3 - Article

VL - 205 (3)

SP - 187

EP - 195

JO - Clinica Chimica Acta

JF - Clinica Chimica Acta

ER -

The determination of asialoglycoforms of serum glycoproteins by lectin blotting with Ricinus communis agglutinin

Abstract

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