The development of a rapid immunobiosensor screening method for the detection of residues of sulphadiazine

Christopher Elliott, Robert Baxter, Joan Crooks, Derek McCaughey

Research output: Contribution to journalArticlepeer-review

40 Citations (Scopus)

Abstract

A rapid imununoassay using an optical biosensor (BIAcore(TM)) for determining the presence of sulphadiazine (SDZ) residues in pig bile was developed. SDZ,cas immobilised onto the surface of a dextran-coated silicon chip and a solution containing SDZ antibody, sample and buffer was injected over the chip surface. The level of antibody binding to the chip was determined after 20 s and the surface of the chip was then regenerated over a 1-min period prior to another sample injection taking place. Standard curves were constructed to allow quantification of SDZ presence in sample. Concentrations ranging from 0 to 10.64 mu g ml(-1) SDZ were detected in bile samples taken from experimentally fed pigs and randomly selected pigs taken from a local slaughterhouse. These results were compared to the concentrations of SDZ detected by high-performance liquid chromatography: in associated tissues. When concentrations in bile exceeded 0.6 mu g ml(-1) SDZ, the corresponding edible tissue was above the maximum residue level (MRL), i.e. 0.1 mu g g(-1) in 13 out of 14 cases. Wizen the bile concentration was less than 0.6 mu ml(-1) the associated tissue concentrations never exceeded rite MRL. This experiment has indicated that biosensor analysis of bile is a highly effective method for detecting violative SDZ residues in meat.
Original languageEnglish
Pages (from-to)19-27
Number of pages9
JournalFOOD AND AGRICULTURAL IMMUNOLOGY
Volume11
Issue number1
Publication statusPublished - Mar 1999

ASJC Scopus subject areas

  • Food Science
  • Chemistry (miscellaneous)
  • Health, Toxicology and Mutagenesis
  • Immunology
  • Toxicology

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