The effect of endothelin 1 on the retinal microvascular pericyte

U. Chakravarthy; T. A. Gardiner; P. Anderson; D. B. Archer; E. R. Trimble

The effect of endothelin 1 on the retinal microvascular pericyte

U. Chakravarthy, T. A. Gardiner, P. Anderson, D. B. Archer, E. R. Trimble

Research output: Contribution to journal › Article › peer-review

Abstract

The effect of the highly vasoactive peptide endothelin 1 (ET1) was tested on bovine retinal microvascular pericytes propagated in vitro. Specific binding of 125I-ET1 to retinal pericytes was documented by autoradiography. ET1 caused contraction of pericytes at a concentration of 0.1 nM which was accompanied by increases in inositol phosphates. Exposure of pericytes to 10 nM ET1 resulted in the aggregation and realignment of muscle-specific actins into bundles which were oriented parallel to the long axis of the cell, and ET1 was also mitogenic to pericytes in the presence of low levels of fetal calf serum. These observations suggest that ET1 may play an important role in endothelial cell-pericyte interactions within the microvasculature of the retina and that it may be involved in the autoregulation of retinal blood flow.

Original language	Undefined/Unknown
Pages (from-to)	241-254
Number of pages	14
Journal	Microvascular Research
Volume	43
Issue number	3
Publication status	Published - 01 May 1992

Bibliographical note

LR: 20031114; JID: 0165035; 0 (Actins); 0 (Endothelins); 0 (Inositol Phosphates); 0 (Mitogens); 0 (Receptors, Cell Surface); 0 (Receptors, Endothelin); ppublish

Keywords

Actins/ultrastructure
Animals
Autoradiography
Cattle
Cell Division/drug effects
Cell Movement/drug effects
Cells/drug effects
Cells, Cultured
Chemotaxis/drug effects
Endothelins/pharmacology
Immunohistochemistry
Inositol Phosphates/biosynthesis/metabolism
Microcirculation/cytology/drug effects
Mitogens/pharmacology
Receptors, Cell Surface/drug effects/metabolism
Receptors, Endothelin
Retinal Vessels/cytology/drug effects
Second Messenger Systems/drug effects

Cite this

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title = "The effect of endothelin 1 on the retinal microvascular pericyte",

abstract = "The effect of the highly vasoactive peptide endothelin 1 (ET1) was tested on bovine retinal microvascular pericytes propagated in vitro. Specific binding of 125I-ET1 to retinal pericytes was documented by autoradiography. ET1 caused contraction of pericytes at a concentration of 0.1 nM which was accompanied by increases in inositol phosphates. Exposure of pericytes to 10 nM ET1 resulted in the aggregation and realignment of muscle-specific actins into bundles which were oriented parallel to the long axis of the cell, and ET1 was also mitogenic to pericytes in the presence of low levels of fetal calf serum. These observations suggest that ET1 may play an important role in endothelial cell-pericyte interactions within the microvasculature of the retina and that it may be involved in the autoregulation of retinal blood flow.",

keywords = "Actins/ultrastructure, Animals, Autoradiography, Cattle, Cell Division/drug effects, Cell Movement/drug effects, Cells/drug effects, Cells, Cultured, Chemotaxis/drug effects, Endothelins/pharmacology, Immunohistochemistry, Inositol Phosphates/biosynthesis/metabolism, Microcirculation/cytology/drug effects, Mitogens/pharmacology, Receptors, Cell Surface/drug effects/metabolism, Receptors, Endothelin, Retinal Vessels/cytology/drug effects, Second Messenger Systems/drug effects",

author = "U. Chakravarthy and Gardiner, {T. A.} and P. Anderson and Archer, {D. B.} and Trimble, {E. R.}",

note = "LR: 20031114; JID: 0165035; 0 (Actins); 0 (Endothelins); 0 (Inositol Phosphates); 0 (Mitogens); 0 (Receptors, Cell Surface); 0 (Receptors, Endothelin); ppublish",

year = "1992",

month = may,

day = "1",

language = "Undefined/Unknown",

volume = "43",

pages = "241--254",

journal = "Microvascular Research",

issn = "0026-2862",

publisher = "Academic Press Inc.",

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TY - JOUR

T1 - The effect of endothelin 1 on the retinal microvascular pericyte

AU - Chakravarthy, U.

AU - Gardiner, T. A.

AU - Anderson, P.

AU - Archer, D. B.

AU - Trimble, E. R.

N1 - LR: 20031114; JID: 0165035; 0 (Actins); 0 (Endothelins); 0 (Inositol Phosphates); 0 (Mitogens); 0 (Receptors, Cell Surface); 0 (Receptors, Endothelin); ppublish

PY - 1992/5/1

Y1 - 1992/5/1

N2 - The effect of the highly vasoactive peptide endothelin 1 (ET1) was tested on bovine retinal microvascular pericytes propagated in vitro. Specific binding of 125I-ET1 to retinal pericytes was documented by autoradiography. ET1 caused contraction of pericytes at a concentration of 0.1 nM which was accompanied by increases in inositol phosphates. Exposure of pericytes to 10 nM ET1 resulted in the aggregation and realignment of muscle-specific actins into bundles which were oriented parallel to the long axis of the cell, and ET1 was also mitogenic to pericytes in the presence of low levels of fetal calf serum. These observations suggest that ET1 may play an important role in endothelial cell-pericyte interactions within the microvasculature of the retina and that it may be involved in the autoregulation of retinal blood flow.

AB - The effect of the highly vasoactive peptide endothelin 1 (ET1) was tested on bovine retinal microvascular pericytes propagated in vitro. Specific binding of 125I-ET1 to retinal pericytes was documented by autoradiography. ET1 caused contraction of pericytes at a concentration of 0.1 nM which was accompanied by increases in inositol phosphates. Exposure of pericytes to 10 nM ET1 resulted in the aggregation and realignment of muscle-specific actins into bundles which were oriented parallel to the long axis of the cell, and ET1 was also mitogenic to pericytes in the presence of low levels of fetal calf serum. These observations suggest that ET1 may play an important role in endothelial cell-pericyte interactions within the microvasculature of the retina and that it may be involved in the autoregulation of retinal blood flow.

KW - Actins/ultrastructure

KW - Animals

KW - Autoradiography

KW - Cattle

KW - Cell Division/drug effects

KW - Cell Movement/drug effects

KW - Cells/drug effects

KW - Cells, Cultured

KW - Chemotaxis/drug effects

KW - Endothelins/pharmacology

KW - Immunohistochemistry

KW - Inositol Phosphates/biosynthesis/metabolism

KW - Microcirculation/cytology/drug effects

KW - Mitogens/pharmacology

KW - Receptors, Cell Surface/drug effects/metabolism

KW - Receptors, Endothelin

KW - Retinal Vessels/cytology/drug effects

KW - Second Messenger Systems/drug effects

M3 - Article

SN - 0026-2862

VL - 43

SP - 241

EP - 254

JO - Microvascular Research

JF - Microvascular Research

IS - 3

ER -