The effect of freeze-drying parameters on the cure characteristics of freeze-dried BSA-loaded silicone elastomer

Christopher McConville, Robert Karl Malcolm, David Woolfson

Research output: Contribution to journalArticlepeer-review

1 Citation (Scopus)

Abstract

To formulate therapeutic proteins into polymeric devices the protein is typically in the solid state, which can be achieved by the process of freeze-drying. However, freeze-drying not only risks denaturing the protein but it can adversely affect the cure characteristics of protein-loaded silicone elastomers. This study demonstrates that a variation in the parameters of the freeze-dryer can significantly affect the residual moisture content of freeze-dried BSA, which in turn has an effect on the bulk density and flow properties of the BSA. The bulk density and flow properties of the BSA subsequently affect the cure characteristics of BSA-loaded silicone elastomers. An increase in the residual moisture content results in the freeze-dried BSA having a decreased bulk density and poor flow properties which can have a detrimental effect on the cure characteristics of a freeze-dried BSA-loaded silicone elastomer.
Original languageEnglish
Pages (from-to) 4402-4408
Number of pages7
JournalJournal of Applied Polymer Science
Volume127
Issue number6
DOIs
Publication statusPublished - 04 Jun 2012

ASJC Scopus subject areas

  • General Chemistry
  • Surfaces, Coatings and Films
  • Polymers and Plastics
  • Materials Chemistry

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