The effect of low level laser irradiation on adult human adipose derived stem cells

B Mvula, T Mathope, T Moore, H Abrahamse

Research output: Contribution to journalArticlepeer-review

95 Citations (Scopus)

Abstract

This study investigated the effect of low level laser irradiation on primary cultures of adult human adipose derived stem cells (ADSC) using a 635-nm diode laser, at 5 J/cm(2) with a power output of 50.2 mW and a power density of 5.5 mW/cm(2). Cellular morphology did not appear to change after irradiation. Using the trypan blue exclusion test, the cellular viability of irradiated cells increased by 1% at 24 h and 1.6% at 48 h but was not statistically significant. However, the increase of cellular viability as measured by ATP luminescence was statistically significant at 48 h (p < 0.05). Proliferation of irradiated cells, measured by optical density, resulted in statistically significant increases in values compared to nonirradiated cells (p < 0.05) at both time points. Western blot analysis and immunocytochemical labeling indicated an increase in the expression of stem cell marker beta1-integrin after irradiation. These results indicate that 5 J/cm(2) of laser irradiation can positively affect human adipose stem cells by increasing cellular viability, proliferation, and expression of beta1-integrin.

Original languageEnglish
Pages (from-to)277-82
Number of pages6
JournalLasers in medical science
Volume23
Issue number3
DOIs
Publication statusPublished - Jul 2008

Keywords

  • Adenosine Triphosphate/metabolism
  • Adipocytes/metabolism
  • Blotting, Western
  • Cell Differentiation/radiation effects
  • Cells, Cultured
  • Humans
  • Integrin beta Chains/metabolism
  • Low-Level Light Therapy/instrumentation
  • Stem Cells/metabolism
  • Trypan Blue

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