The effects of extracellular calcium-sensing receptor ligands on the contractility of pregnant human myometrium ni vitro

Denis J. Crankshaw*, Marc J. Pistilli, Yvonne M. O'Brien, Eva M. Sweeney, Peter Dockery, Alison C. Holloway, John J. Morrison

*Corresponding author for this work

Research output: Contribution to journalArticle

5 Citations (Scopus)

Abstract

Ligands for extracellular calcium-sensing (CaS) receptors inhibit oxytocin-induced contractions of the rat's uterus. In this study, we investigated whether the CaS receptor ligands calindol, cinacalcet, and calhex 231 have similar effects on pregnant human myometrium. We compared their effects to those of the calcium-channel blocker nifedipine. In conventional concentration-effect experiments, both the mean contractile force (MCF) and the maximum amplitude of contractions induced by 1 nmol/L oxytocin were inhibited by nifedipine. Calindol and cinacalcet were ineffective as inhibitors, while calhex-231 produced partial inhibition. When single 10 μmol/L doses were applied calhex-231 produced a slowly developing inhibition, reducing the MCF to 38%, and amplitude to 34%, of vehicle controls after 1 hour. In similar experiments, calindol was ineffective while cinacalcet weakly inhibited only the amplitude. Immunohistochemistry revealed sparse expression of CaS receptors in pregnant human myometrium.

Original languageEnglish
Pages (from-to)882-890
Number of pages9
JournalReproductive Sciences
Volume20
Issue number8
Early online date03 Jan 2013
DOIs
Publication statusPublished - 01 Aug 2013
Externally publishedYes

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Keywords

  • calcium-sensing receptors
  • calhex-231
  • calindol
  • cinacalcet
  • human myometrium
  • tocolysis

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