The EuroChimerism concept for a standardized approach to chimerism analysis after allogeneic stem cell transplantation

T. Lion; F. Watzinger; S. Preuner; H. Kreyenberg; M. Tilanus; R. De Weger; J. Van Loon; L. De Vries; H. Cavé; C. Acquaviva; M. Lawler; M. Crampe; A. Serra; B. Saglio; F. Colnaghi; A. Biondi; J. J.M. Van Dongen; M. Van Der Burg; M. Gonzalez; M. Alcoceba; G. Barbany; M. Hermanson; E. Roosnek; C. Steward; J. Harvey; F. Frommlet; P. Bader

doi:10.1038/leu.2012.66

The EuroChimerism concept for a standardized approach to chimerism analysis after allogeneic stem cell transplantation

T. Lion^*
, F. Watzinger
, S. Preuner
, H. Kreyenberg
, M. Tilanus
, R. De Weger
, J. Van Loon
, L. De Vries
, H. Cavé
, C. Acquaviva
, M. Lawler
, M. Crampe
, A. Serra
, B. Saglio
, F. Colnaghi
, A. Biondi
, J. J.M. Van Dongen
, M. Van Der Burg
, M. Gonzalez
, M. Alcoceba

G. Barbany, M. Hermanson, E. Roosnek, C. Steward, J. Harvey, F. Frommlet, P. Bader

^*Corresponding author for this work

School of Medicine, Dentistry and Biomedical Sciences

Research output: Contribution to journal › Article › peer-review

93 Citations (Scopus)

Abstract

Hematopoietic stem cell transplantation is becoming an increasingly important approach to treatment of different malignant and non-malignant disorders. There is thus growing demand for diagnostic assays permitting the surveillance of donor/recipient chimerism posttransplant. Current techniques are heterogeneous, rendering uniform evaluation and comparison of diagnostic results between centers difficult. Leading laboratories from 10 European countries have therefore performed a collaborative study supported by a European grant, the EuroChimerism Concerted Action, with the aim to develop a standardized diagnostic methodology for the detection and monitoring of chimerism in patients undergoing allogeneic stem cell transplantation. Following extensive analysis of a large set of microsatellite/short tandem repeat (STR) loci, the EuroChimerism (EUC) panel comprising 13 STR markers was established with the aim to optimally meet the specific requirements of quantitative chimerism analysis. Based on highly stringent selection criteria, the EUC panel provides multiple informative markers in any transplant setting. The standardized STR-PCR tests permit detection of donor-or recipient-derived cells at a sensitivity ranging between 0.8 and 1.6%. Moreover, the EUC assay facilitates accurate and reproducible quantification of donor and recipient hematopoietic cells. Wide use of the European-harmonized protocol for chimerism analysis presented will provide a basis for optimal diagnostic support and timely treatment decisions.

Original language	English
Pages (from-to)	1821-1828
Number of pages	8
Journal	Leukemia
Volume	26
Issue number	8
DOIs	https://doi.org/10.1038/leu.2012.66
Publication status	Published - Aug 2012

Bibliographical note

Funding Information:
We are grateful to the EU officers who have supervised and supported the project, including particularly Jaime Renart, Cornelius Schmaltz, Maria-José Vidal-Ragout and Micheline Divaris-Lambert. Moreover, we greatly appreciate the financial administrative help of Claudia Hochweis, Stefan Grünert, Alexandra Lidy and Karla Valdés-Rodriguez, the secretarial support of Ingrid Kerschberger and Nicole Reisinger at the coordinating center (CCRI) and the contributions of numerous technicians at the participating centers. The EuroChimerism Concerted Action (full project title: Diagnostic approaches to chimerism testing after allogeneic stem cell transplantation for early detection of graft rejection and relapse: technical development, standardization and European-coordinated clinical implementation) was carried out with financial support from the European commission within the call Quality of Life and Management of Living Resources, Fifth Framework Program, project No QLG1-CT-2002-01485.

UN SDGs

This output contributes to the following UN Sustainable Development Goals (SDGs)

SDG 3 Good Health and Well-being

Keywords

alleles
microsatellites
PCR
short tandem repeats

ASJC Scopus subject areas

Hematology
Oncology
Cancer Research

Access to Document

10.1038/leu.2012.66

Cite this

Lion, T., Watzinger, F., Preuner, S., Kreyenberg, H., Tilanus, M., De Weger, R., Van Loon, J., De Vries, L., Cavé, H., Acquaviva, C., Lawler, M., Crampe, M., Serra, A., Saglio, B., Colnaghi, F., Biondi, A., Van Dongen, J. J. M., Van Der Burg, M., Gonzalez, M., ... Bader, P. (2012). The EuroChimerism concept for a standardized approach to chimerism analysis after allogeneic stem cell transplantation. Leukemia, 26(8), 1821-1828. https://doi.org/10.1038/leu.2012.66

@article{f604d642c2f84a05953a898c22240ab9,

title = "The EuroChimerism concept for a standardized approach to chimerism analysis after allogeneic stem cell transplantation",

abstract = "Hematopoietic stem cell transplantation is becoming an increasingly important approach to treatment of different malignant and non-malignant disorders. There is thus growing demand for diagnostic assays permitting the surveillance of donor/recipient chimerism posttransplant. Current techniques are heterogeneous, rendering uniform evaluation and comparison of diagnostic results between centers difficult. Leading laboratories from 10 European countries have therefore performed a collaborative study supported by a European grant, the EuroChimerism Concerted Action, with the aim to develop a standardized diagnostic methodology for the detection and monitoring of chimerism in patients undergoing allogeneic stem cell transplantation. Following extensive analysis of a large set of microsatellite/short tandem repeat (STR) loci, the EuroChimerism (EUC) panel comprising 13 STR markers was established with the aim to optimally meet the specific requirements of quantitative chimerism analysis. Based on highly stringent selection criteria, the EUC panel provides multiple informative markers in any transplant setting. The standardized STR-PCR tests permit detection of donor-or recipient-derived cells at a sensitivity ranging between 0.8 and 1.6\%. Moreover, the EUC assay facilitates accurate and reproducible quantification of donor and recipient hematopoietic cells. Wide use of the European-harmonized protocol for chimerism analysis presented will provide a basis for optimal diagnostic support and timely treatment decisions.",

keywords = "alleles, microsatellites, PCR, short tandem repeats",

author = "T. Lion and F. Watzinger and S. Preuner and H. Kreyenberg and M. Tilanus and \{De Weger\}, R. and \{Van Loon\}, J. and \{De Vries\}, L. and H. Cav{\'e} and C. Acquaviva and M. Lawler and M. Crampe and A. Serra and B. Saglio and F. Colnaghi and A. Biondi and \{Van Dongen\}, \{J. J.M.\} and \{Van Der Burg\}, M. and M. Gonzalez and M. Alcoceba and G. Barbany and M. Hermanson and E. Roosnek and C. Steward and J. Harvey and F. Frommlet and P. Bader",

note = "Funding Information: We are grateful to the EU officers who have supervised and supported the project, including particularly Jaime Renart, Cornelius Schmaltz, Maria-Jos{\'e} Vidal-Ragout and Micheline Divaris-Lambert. Moreover, we greatly appreciate the financial administrative help of Claudia Hochweis, Stefan Gr{\"u}nert, Alexandra Lidy and Karla Vald{\'e}s-Rodriguez, the secretarial support of Ingrid Kerschberger and Nicole Reisinger at the coordinating center (CCRI) and the contributions of numerous technicians at the participating centers. The EuroChimerism Concerted Action (full project title: Diagnostic approaches to chimerism testing after allogeneic stem cell transplantation for early detection of graft rejection and relapse: technical development, standardization and European-coordinated clinical implementation) was carried out with financial support from the European commission within the call Quality of Life and Management of Living Resources, Fifth Framework Program, project No QLG1-CT-2002-01485.",

year = "2012",

month = aug,

doi = "10.1038/leu.2012.66",

language = "English",

volume = "26",

pages = "1821--1828",

journal = "Leukemia",

issn = "0887-6924",

publisher = "Springer Nature",

number = "8",

}

Lion, T, Watzinger, F, Preuner, S, Kreyenberg, H, Tilanus, M, De Weger, R, Van Loon, J, De Vries, L, Cavé, H, Acquaviva, C, Lawler, M, Crampe, M, Serra, A, Saglio, B, Colnaghi, F, Biondi, A, Van Dongen, JJM, Van Der Burg, M, Gonzalez, M, Alcoceba, M, Barbany, G, Hermanson, M, Roosnek, E, Steward, C, Harvey, J, Frommlet, F & Bader, P 2012, 'The EuroChimerism concept for a standardized approach to chimerism analysis after allogeneic stem cell transplantation', Leukemia, vol. 26, no. 8, pp. 1821-1828. https://doi.org/10.1038/leu.2012.66

TY - JOUR

T1 - The EuroChimerism concept for a standardized approach to chimerism analysis after allogeneic stem cell transplantation

AU - Lion, T.

AU - Watzinger, F.

AU - Preuner, S.

AU - Kreyenberg, H.

AU - Tilanus, M.

AU - De Weger, R.

AU - Van Loon, J.

AU - De Vries, L.

AU - Cavé, H.

AU - Acquaviva, C.

AU - Lawler, M.

AU - Crampe, M.

AU - Serra, A.

AU - Saglio, B.

AU - Colnaghi, F.

AU - Biondi, A.

AU - Van Dongen, J. J.M.

AU - Van Der Burg, M.

AU - Gonzalez, M.

AU - Alcoceba, M.

AU - Barbany, G.

AU - Hermanson, M.

AU - Roosnek, E.

AU - Steward, C.

AU - Harvey, J.

AU - Frommlet, F.

AU - Bader, P.

N1 - Funding Information: We are grateful to the EU officers who have supervised and supported the project, including particularly Jaime Renart, Cornelius Schmaltz, Maria-José Vidal-Ragout and Micheline Divaris-Lambert. Moreover, we greatly appreciate the financial administrative help of Claudia Hochweis, Stefan Grünert, Alexandra Lidy and Karla Valdés-Rodriguez, the secretarial support of Ingrid Kerschberger and Nicole Reisinger at the coordinating center (CCRI) and the contributions of numerous technicians at the participating centers. The EuroChimerism Concerted Action (full project title: Diagnostic approaches to chimerism testing after allogeneic stem cell transplantation for early detection of graft rejection and relapse: technical development, standardization and European-coordinated clinical implementation) was carried out with financial support from the European commission within the call Quality of Life and Management of Living Resources, Fifth Framework Program, project No QLG1-CT-2002-01485.

PY - 2012/8

Y1 - 2012/8

N2 - Hematopoietic stem cell transplantation is becoming an increasingly important approach to treatment of different malignant and non-malignant disorders. There is thus growing demand for diagnostic assays permitting the surveillance of donor/recipient chimerism posttransplant. Current techniques are heterogeneous, rendering uniform evaluation and comparison of diagnostic results between centers difficult. Leading laboratories from 10 European countries have therefore performed a collaborative study supported by a European grant, the EuroChimerism Concerted Action, with the aim to develop a standardized diagnostic methodology for the detection and monitoring of chimerism in patients undergoing allogeneic stem cell transplantation. Following extensive analysis of a large set of microsatellite/short tandem repeat (STR) loci, the EuroChimerism (EUC) panel comprising 13 STR markers was established with the aim to optimally meet the specific requirements of quantitative chimerism analysis. Based on highly stringent selection criteria, the EUC panel provides multiple informative markers in any transplant setting. The standardized STR-PCR tests permit detection of donor-or recipient-derived cells at a sensitivity ranging between 0.8 and 1.6%. Moreover, the EUC assay facilitates accurate and reproducible quantification of donor and recipient hematopoietic cells. Wide use of the European-harmonized protocol for chimerism analysis presented will provide a basis for optimal diagnostic support and timely treatment decisions.

AB - Hematopoietic stem cell transplantation is becoming an increasingly important approach to treatment of different malignant and non-malignant disorders. There is thus growing demand for diagnostic assays permitting the surveillance of donor/recipient chimerism posttransplant. Current techniques are heterogeneous, rendering uniform evaluation and comparison of diagnostic results between centers difficult. Leading laboratories from 10 European countries have therefore performed a collaborative study supported by a European grant, the EuroChimerism Concerted Action, with the aim to develop a standardized diagnostic methodology for the detection and monitoring of chimerism in patients undergoing allogeneic stem cell transplantation. Following extensive analysis of a large set of microsatellite/short tandem repeat (STR) loci, the EuroChimerism (EUC) panel comprising 13 STR markers was established with the aim to optimally meet the specific requirements of quantitative chimerism analysis. Based on highly stringent selection criteria, the EUC panel provides multiple informative markers in any transplant setting. The standardized STR-PCR tests permit detection of donor-or recipient-derived cells at a sensitivity ranging between 0.8 and 1.6%. Moreover, the EUC assay facilitates accurate and reproducible quantification of donor and recipient hematopoietic cells. Wide use of the European-harmonized protocol for chimerism analysis presented will provide a basis for optimal diagnostic support and timely treatment decisions.

KW - alleles

KW - microsatellites

KW - PCR

KW - short tandem repeats

UR - https://www.scopus.com/pages/publications/84864876080

U2 - 10.1038/leu.2012.66

DO - 10.1038/leu.2012.66

M3 - Article

C2 - 22395360

AN - SCOPUS:84864876080

SN - 0887-6924

VL - 26

SP - 1821

EP - 1828

JO - Leukemia

JF - Leukemia

IS - 8

ER -

The EuroChimerism concept for a standardized approach to chimerism analysis after allogeneic stem cell transplantation

Abstract

Bibliographical note

UN SDGs

Keywords

ASJC Scopus subject areas

Access to Document

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