The Burkholderia cenocepacia sensor kinase hybrid AtsR is a global regulator modulating quorum-sensing signalling

Daniel F Aubert, Eoin P O'Grady, Mohamad A Hamad, Pamela A Sokol, Miguel A Valvano

Research output: Contribution to journalArticlepeer-review

29 Citations (Scopus)


Burkholderia cenocepacia is commonly found in the environment and also as an important opportunistic pathogen infecting patients with cystic fibrosis. Successful infection by this bacterium requires coordinated expression of virulence factors, which is achieved through different quorum sensing (QS) regulatory systems. Biofilm formation and Type 6 secretion system (T6SS) expression in B. cenocepacia K56-2 are positively regulated by QS and negatively regulated by the sensor kinase hybrid AtsR. This study reveals that in addition to affecting biofilm and T6SS activity, the deletion of atsR in B. cenocepacia leads to overproduction of other QS-regulated virulence determinants including proteases and swarming motility. Expression of the QS genes, cepIR and cciIR, was upregulated in the ΔatsR mutant and resulted in early and increased N-acylhomoserine lactone (AHL) production, suggesting that AtsR plays a role in controlling the timing and fine-tuning of virulence gene expression by modulating QS signalling. Furthermore, a ΔatsRΔcepIΔcciImutant could partially upregulate the same virulence determinants indicating that AtsR also modulates the expression of virulence genes by a second mechanism, independently of any AHL production. Together, our results strongly suggest that AtsR is a global virulence regulator in B. cenocepacia.
Original languageEnglish
Pages (from-to)372-385
Number of pages14
JournalEnvironmental Microbiology
Issue number2
Early online date26 Jul 2012
Publication statusPublished - 28 Feb 2013

ASJC Scopus subject areas

  • Ecology, Evolution, Behavior and Systematics
  • Microbiology


Dive into the research topics of 'The <i>Burkholderia cenocepacia </i>sensor kinase hybrid AtsR is a global regulator modulating quorum-sensing signalling'. Together they form a unique fingerprint.

Cite this