The impact of sperm DNA damage in assisted conception and beyond: recent advances in diagnosis and treatment

Sheena Lewis, R. John Aitken, Sarah J Conner, Geoffry De Luliis, Donald P. Evenson, Ralph Henkel, Aleksander Giwercman, Parviz Gharagozloo

Research output: Contribution to journalLiterature review

114 Citations (Scopus)

Abstract

Sperm DNA damage is a useful biomarker for male infertility diagnosis and prediction of assisted reproduction outcomes. It is associated with reduced fertilization rates, embryo quality and pregnancy rates, and higher rates of spontaneous miscarriage and childhood diseases. This review provides a synopsis of the most recent studies from each of the authors, all of whom have major track records in the field of sperm DNA damage in the clinical setting. It explores current laboratory tests and the accumulating body of knowledge concerning the relationship between sperm DNA damage and clinical outcomes. The paper proceeds to discuss the strengths, weaknesses and clinical applicability of current sperm DNA tests. Next, the biological significance of DNA damage in the male germ line is considered. Finally, as sperm DNA damage is often the result of oxidative stress in the male reproductive tract, the potential contribution of antioxidant therapy in the clinical management of this condition is discussed. DNA damage in human spermatozoa is an important attribute of semen quality. It should be part of the clinical work up and properly controlled trials addressing the effectiveness of antioxidant therapy should be undertaken as a matter of urgency.

Sperm DNA damage is a useful biomarker for male infertility diagnosis and prediction of assisted reproduction outcomes. It is associated with reduced fertilization rates, embryo quality and pregnancy rates, and higher rates of spontaneous miscarriage and childhood diseases. With all of these fertility check points, it shows more promise than conventional semen parameters from a diagnostic perspective. Despite this, few infertility clinics use it routinely. This review provides a synopsis of the most recent studies from each of the authors, all of whom have major track records in the field of sperm DNA damage in the clinical setting. It explores current laboratory tests and the accumulating body of knowledge concerning the relationship between sperm DNA damage and clinical outcomes. The paper proceeds to discuss the strengths and weaknesses and clinical applicability of current sperm DNA fragmentation tests. Next, the biological significance of DNA damage in the male germ line is considered. Finally, as sperm DNA damage is often the result of increased oxidative stress in the male reproductive tract, the potential contribution of antioxidant therapy in the clinical management of this condition is discussed. As those working in this field of clinical research, we conclude that DNA damage in human spermatozoa is an important attribute of semen quality which should be carefully assessed in the clinical work up of infertile couples and that properly controlled trials addressing the effectiveness of antioxidant therapy should be undertaken as a matter of urgency
Original languageEnglish
Pages (from-to)325-337
JournalReproductive BioMedicine Online
Volume27
Issue number4
Early online date11 Jul 2013
DOIs
Publication statusUnpublished - Oct 2013

Fingerprint

DNA Damage
Spermatozoa
Antioxidants
Semen Analysis
Male Infertility
Spontaneous Abortion
Pregnancy Rate
Fertilization
Germ Cells
Reproduction
Oxidative Stress
Embryonic Structures
Biomarkers
DNA Fragmentation
Therapeutics
Semen
Infertility
Fertility
DNA

Keywords

  • antioxidant therapy, assisted reproduction, DNA fragmentation, Male infertility, oxidized bases, spermatozoa

Cite this

Lewis, S., Aitken, R. J., Conner, S. J., De Luliis, G., Evenson, D. P., Henkel, R., ... Gharagozloo, P. (2013). The impact of sperm DNA damage in assisted conception and beyond: recent advances in diagnosis and treatment. Unpublished. https://doi.org/10.1016/j.rbmo.2013.06.014
Lewis, Sheena ; Aitken, R. John ; Conner, Sarah J ; De Luliis, Geoffry ; Evenson, Donald P. ; Henkel, Ralph ; Giwercman, Aleksander ; Gharagozloo, Parviz. / The impact of sperm DNA damage in assisted conception and beyond: recent advances in diagnosis and treatment. In: Reproductive BioMedicine Online. 2013 ; Vol. 27, No. 4. pp. 325-337.
@article{cfe7495ae0bb4b94a5dcd61be42469d4,
title = "The impact of sperm DNA damage in assisted conception and beyond: recent advances in diagnosis and treatment",
abstract = "Sperm DNA damage is a useful biomarker for male infertility diagnosis and prediction of assisted reproduction outcomes. It is associated with reduced fertilization rates, embryo quality and pregnancy rates, and higher rates of spontaneous miscarriage and childhood diseases. This review provides a synopsis of the most recent studies from each of the authors, all of whom have major track records in the field of sperm DNA damage in the clinical setting. It explores current laboratory tests and the accumulating body of knowledge concerning the relationship between sperm DNA damage and clinical outcomes. The paper proceeds to discuss the strengths, weaknesses and clinical applicability of current sperm DNA tests. Next, the biological significance of DNA damage in the male germ line is considered. Finally, as sperm DNA damage is often the result of oxidative stress in the male reproductive tract, the potential contribution of antioxidant therapy in the clinical management of this condition is discussed. DNA damage in human spermatozoa is an important attribute of semen quality. It should be part of the clinical work up and properly controlled trials addressing the effectiveness of antioxidant therapy should be undertaken as a matter of urgency.Sperm DNA damage is a useful biomarker for male infertility diagnosis and prediction of assisted reproduction outcomes. It is associated with reduced fertilization rates, embryo quality and pregnancy rates, and higher rates of spontaneous miscarriage and childhood diseases. With all of these fertility check points, it shows more promise than conventional semen parameters from a diagnostic perspective. Despite this, few infertility clinics use it routinely. This review provides a synopsis of the most recent studies from each of the authors, all of whom have major track records in the field of sperm DNA damage in the clinical setting. It explores current laboratory tests and the accumulating body of knowledge concerning the relationship between sperm DNA damage and clinical outcomes. The paper proceeds to discuss the strengths and weaknesses and clinical applicability of current sperm DNA fragmentation tests. Next, the biological significance of DNA damage in the male germ line is considered. Finally, as sperm DNA damage is often the result of increased oxidative stress in the male reproductive tract, the potential contribution of antioxidant therapy in the clinical management of this condition is discussed. As those working in this field of clinical research, we conclude that DNA damage in human spermatozoa is an important attribute of semen quality which should be carefully assessed in the clinical work up of infertile couples and that properly controlled trials addressing the effectiveness of antioxidant therapy should be undertaken as a matter of urgency",
keywords = "antioxidant therapy, assisted reproduction, DNA fragmentation, Male infertility, oxidized bases, spermatozoa",
author = "Sheena Lewis and Aitken, {R. John} and Conner, {Sarah J} and {De Luliis}, Geoffry and Evenson, {Donald P.} and Ralph Henkel and Aleksander Giwercman and Parviz Gharagozloo",
year = "2013",
month = "10",
doi = "10.1016/j.rbmo.2013.06.014",
language = "English",
volume = "27",
pages = "325--337",
journal = "Reproductive BioMedicine Online",
issn = "1472-6483",
publisher = "Elsevier",
number = "4",

}

Lewis, S, Aitken, RJ, Conner, SJ, De Luliis, G, Evenson, DP, Henkel, R, Giwercman, A & Gharagozloo, P 2013, 'The impact of sperm DNA damage in assisted conception and beyond: recent advances in diagnosis and treatment', Reproductive BioMedicine Online, vol. 27, no. 4, pp. 325-337. https://doi.org/10.1016/j.rbmo.2013.06.014

The impact of sperm DNA damage in assisted conception and beyond: recent advances in diagnosis and treatment. / Lewis, Sheena; Aitken, R. John ; Conner, Sarah J; De Luliis, Geoffry ; Evenson, Donald P. ; Henkel, Ralph ; Giwercman, Aleksander ; Gharagozloo, Parviz.

In: Reproductive BioMedicine Online, Vol. 27, No. 4, 10.2013, p. 325-337.

Research output: Contribution to journalLiterature review

TY - JOUR

T1 - The impact of sperm DNA damage in assisted conception and beyond: recent advances in diagnosis and treatment

AU - Lewis, Sheena

AU - Aitken, R. John

AU - Conner, Sarah J

AU - De Luliis, Geoffry

AU - Evenson, Donald P.

AU - Henkel, Ralph

AU - Giwercman, Aleksander

AU - Gharagozloo, Parviz

PY - 2013/10

Y1 - 2013/10

N2 - Sperm DNA damage is a useful biomarker for male infertility diagnosis and prediction of assisted reproduction outcomes. It is associated with reduced fertilization rates, embryo quality and pregnancy rates, and higher rates of spontaneous miscarriage and childhood diseases. This review provides a synopsis of the most recent studies from each of the authors, all of whom have major track records in the field of sperm DNA damage in the clinical setting. It explores current laboratory tests and the accumulating body of knowledge concerning the relationship between sperm DNA damage and clinical outcomes. The paper proceeds to discuss the strengths, weaknesses and clinical applicability of current sperm DNA tests. Next, the biological significance of DNA damage in the male germ line is considered. Finally, as sperm DNA damage is often the result of oxidative stress in the male reproductive tract, the potential contribution of antioxidant therapy in the clinical management of this condition is discussed. DNA damage in human spermatozoa is an important attribute of semen quality. It should be part of the clinical work up and properly controlled trials addressing the effectiveness of antioxidant therapy should be undertaken as a matter of urgency.Sperm DNA damage is a useful biomarker for male infertility diagnosis and prediction of assisted reproduction outcomes. It is associated with reduced fertilization rates, embryo quality and pregnancy rates, and higher rates of spontaneous miscarriage and childhood diseases. With all of these fertility check points, it shows more promise than conventional semen parameters from a diagnostic perspective. Despite this, few infertility clinics use it routinely. This review provides a synopsis of the most recent studies from each of the authors, all of whom have major track records in the field of sperm DNA damage in the clinical setting. It explores current laboratory tests and the accumulating body of knowledge concerning the relationship between sperm DNA damage and clinical outcomes. The paper proceeds to discuss the strengths and weaknesses and clinical applicability of current sperm DNA fragmentation tests. Next, the biological significance of DNA damage in the male germ line is considered. Finally, as sperm DNA damage is often the result of increased oxidative stress in the male reproductive tract, the potential contribution of antioxidant therapy in the clinical management of this condition is discussed. As those working in this field of clinical research, we conclude that DNA damage in human spermatozoa is an important attribute of semen quality which should be carefully assessed in the clinical work up of infertile couples and that properly controlled trials addressing the effectiveness of antioxidant therapy should be undertaken as a matter of urgency

AB - Sperm DNA damage is a useful biomarker for male infertility diagnosis and prediction of assisted reproduction outcomes. It is associated with reduced fertilization rates, embryo quality and pregnancy rates, and higher rates of spontaneous miscarriage and childhood diseases. This review provides a synopsis of the most recent studies from each of the authors, all of whom have major track records in the field of sperm DNA damage in the clinical setting. It explores current laboratory tests and the accumulating body of knowledge concerning the relationship between sperm DNA damage and clinical outcomes. The paper proceeds to discuss the strengths, weaknesses and clinical applicability of current sperm DNA tests. Next, the biological significance of DNA damage in the male germ line is considered. Finally, as sperm DNA damage is often the result of oxidative stress in the male reproductive tract, the potential contribution of antioxidant therapy in the clinical management of this condition is discussed. DNA damage in human spermatozoa is an important attribute of semen quality. It should be part of the clinical work up and properly controlled trials addressing the effectiveness of antioxidant therapy should be undertaken as a matter of urgency.Sperm DNA damage is a useful biomarker for male infertility diagnosis and prediction of assisted reproduction outcomes. It is associated with reduced fertilization rates, embryo quality and pregnancy rates, and higher rates of spontaneous miscarriage and childhood diseases. With all of these fertility check points, it shows more promise than conventional semen parameters from a diagnostic perspective. Despite this, few infertility clinics use it routinely. This review provides a synopsis of the most recent studies from each of the authors, all of whom have major track records in the field of sperm DNA damage in the clinical setting. It explores current laboratory tests and the accumulating body of knowledge concerning the relationship between sperm DNA damage and clinical outcomes. The paper proceeds to discuss the strengths and weaknesses and clinical applicability of current sperm DNA fragmentation tests. Next, the biological significance of DNA damage in the male germ line is considered. Finally, as sperm DNA damage is often the result of increased oxidative stress in the male reproductive tract, the potential contribution of antioxidant therapy in the clinical management of this condition is discussed. As those working in this field of clinical research, we conclude that DNA damage in human spermatozoa is an important attribute of semen quality which should be carefully assessed in the clinical work up of infertile couples and that properly controlled trials addressing the effectiveness of antioxidant therapy should be undertaken as a matter of urgency

KW - antioxidant therapy, assisted reproduction, DNA fragmentation, Male infertility, oxidized bases, spermatozoa

U2 - 10.1016/j.rbmo.2013.06.014

DO - 10.1016/j.rbmo.2013.06.014

M3 - Literature review

VL - 27

SP - 325

EP - 337

JO - Reproductive BioMedicine Online

JF - Reproductive BioMedicine Online

SN - 1472-6483

IS - 4

ER -