The Legionella pneumophila Dot/Icm-secreted effector PlcC/CegC1 together with PlcA and PlcB promotes virulence and belongs to a novel zinc metallophospholipase C family present in bacteria and fungi

Philipp Aurass; Maren Schlegel; Omar Metwally; Clare R. Harding; Gunnar N. Schroeder; Gad Frankel; Antje Flieger

doi:10.1074/jbc.M112.426049

The Legionella pneumophila Dot/Icm-secreted effector PlcC/CegC1 together with PlcA and PlcB promotes virulence and belongs to a novel zinc metallophospholipase C family present in bacteria and fungi

Philipp Aurass, Maren Schlegel, Omar Metwally, Clare R. Harding, Gunnar N. Schroeder, Gad Frankel, Antje Flieger^*

^*Corresponding author for this work

Research output: Contribution to journal › Article

28 Citations (Scopus)

Abstract

Background: It is unclear whether Legionella pneumophila possesses phospholipase C (PLC) activity and thereby generates 1,2-diacylglycerol.

Results: L. pneumophila possesses three secreted enzymes with PLC activity, PlcA, PlcB, and PlcC, and a plcABC mutant was attenuated in host killing.

Conclusion: L. pneumophila encodes three members of a novel PLC family contributing to virulence.

Significance: We determined PLC activity for L. pneumophila and defined the characteristics of a novel PLC family present in Legionella, Pseudomonas, and fungi.

Original language	English
Pages (from-to)	11080-11092
Number of pages	13
Journal	Journal of Biological Chemistry
Volume	288
Issue number	16
Early online date	01 Mar 2013
DOIs	https://doi.org/10.1074/jbc.M112.426049
Publication status	Published - 19 Apr 2013
Externally published	Yes

ASJC Scopus subject areas

Biochemistry
Cell Biology
Molecular Biology

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Aurass, P., Schlegel, M., Metwally, O., Harding, C. R., Schroeder, G. N., Frankel, G., & Flieger, A. (2013). The Legionella pneumophila Dot/Icm-secreted effector PlcC/CegC1 together with PlcA and PlcB promotes virulence and belongs to a novel zinc metallophospholipase C family present in bacteria and fungi. Journal of Biological Chemistry, 288(16), 11080-11092. https://doi.org/10.1074/jbc.M112.426049

Aurass, Philipp ; Schlegel, Maren ; Metwally, Omar ; Harding, Clare R. ; Schroeder, Gunnar N. ; Frankel, Gad ; Flieger, Antje. / The Legionella pneumophila Dot/Icm-secreted effector PlcC/CegC1 together with PlcA and PlcB promotes virulence and belongs to a novel zinc metallophospholipase C family present in bacteria and fungi. In: Journal of Biological Chemistry. 2013 ; Vol. 288, No. 16. pp. 11080-11092.

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title = "The Legionella pneumophila Dot/Icm-secreted effector PlcC/CegC1 together with PlcA and PlcB promotes virulence and belongs to a novel zinc metallophospholipase C family present in bacteria and fungi",

abstract = "Background: It is unclear whether Legionella pneumophila possesses phospholipase C (PLC) activity and thereby generates 1,2-diacylglycerol. Results: L. pneumophila possesses three secreted enzymes with PLC activity, PlcA, PlcB, and PlcC, and a plcABC mutant was attenuated in host killing. Conclusion: L. pneumophila encodes three members of a novel PLC family contributing to virulence. Significance: We determined PLC activity for L. pneumophila and defined the characteristics of a novel PLC family present in Legionella, Pseudomonas, and fungi.",

author = "Philipp Aurass and Maren Schlegel and Omar Metwally and Harding, {Clare R.} and Schroeder, {Gunnar N.} and Gad Frankel and Antje Flieger",

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Aurass, P, Schlegel, M, Metwally, O, Harding, CR, Schroeder, GN, Frankel, G & Flieger, A 2013, 'The Legionella pneumophila Dot/Icm-secreted effector PlcC/CegC1 together with PlcA and PlcB promotes virulence and belongs to a novel zinc metallophospholipase C family present in bacteria and fungi', Journal of Biological Chemistry, vol. 288, no. 16, pp. 11080-11092. https://doi.org/10.1074/jbc.M112.426049

The Legionella pneumophila Dot/Icm-secreted effector PlcC/CegC1 together with PlcA and PlcB promotes virulence and belongs to a novel zinc metallophospholipase C family present in bacteria and fungi. / Aurass, Philipp; Schlegel, Maren; Metwally, Omar; Harding, Clare R.; Schroeder, Gunnar N.; Frankel, Gad; Flieger, Antje.

In: Journal of Biological Chemistry, Vol. 288, No. 16, 19.04.2013, p. 11080-11092.

Research output: Contribution to journal › Article

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AU - Aurass, Philipp

AU - Schlegel, Maren

AU - Metwally, Omar

AU - Harding, Clare R.

AU - Schroeder, Gunnar N.

AU - Frankel, Gad

AU - Flieger, Antje

PY - 2013/4/19

Y1 - 2013/4/19

N2 - Background: It is unclear whether Legionella pneumophila possesses phospholipase C (PLC) activity and thereby generates 1,2-diacylglycerol. Results: L. pneumophila possesses three secreted enzymes with PLC activity, PlcA, PlcB, and PlcC, and a plcABC mutant was attenuated in host killing. Conclusion: L. pneumophila encodes three members of a novel PLC family contributing to virulence. Significance: We determined PLC activity for L. pneumophila and defined the characteristics of a novel PLC family present in Legionella, Pseudomonas, and fungi.

AB - Background: It is unclear whether Legionella pneumophila possesses phospholipase C (PLC) activity and thereby generates 1,2-diacylglycerol. Results: L. pneumophila possesses three secreted enzymes with PLC activity, PlcA, PlcB, and PlcC, and a plcABC mutant was attenuated in host killing. Conclusion: L. pneumophila encodes three members of a novel PLC family contributing to virulence. Significance: We determined PLC activity for L. pneumophila and defined the characteristics of a novel PLC family present in Legionella, Pseudomonas, and fungi.

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Aurass P, Schlegel M, Metwally O, Harding CR, Schroeder GN, Frankel G et al. The Legionella pneumophila Dot/Icm-secreted effector PlcC/CegC1 together with PlcA and PlcB promotes virulence and belongs to a novel zinc metallophospholipase C family present in bacteria and fungi. Journal of Biological Chemistry. 2013 Apr 19;288(16):11080-11092. https://doi.org/10.1074/jbc.M112.426049