The mutagenesis of a single site for enhancing or reversing the enantio- or regiopreference of cyclohexanone monooxygenases

Yujing Hu; Weihua Xu; Chenggong Hui; Jian  Xu; Xianfu Lin; Meilan Huang; Qi Wu

doi:10.1039/D0CC03721D

The mutagenesis of a single site for enhancing or reversing the enantio- or regiopreference of cyclohexanone monooxygenases

Yujing Hu, Weihua Xu, Chenggong Hui, Jian Xu, Xianfu Lin, Meilan Huang^*, Qi Wu^*

^*Corresponding author for this work

School of Chemistry and Chemical Engineering

Research output: Contribution to journal › Article › peer-review

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Abstract

The mutagenesis of a “second sphere” switch residue of CHMOAcineto could control its enantio- and regiopreference. Replacing phenylalanine (F) at position 277 of CHMOAcineto into larger tryptophan (W) enabled a significant enhancement of enantio- or regioselectivity toward structurally diverse substrates, moreover, a complete reversal of enantio- or regiopreference was realized by mutating F277 into a range of smaller amino acids (A/C/D/E/G/H/I/K/L/M/N/P/Q/R/S/T/V).

Original language	English
Pages (from-to)	9356-9359
Number of pages	4
Journal	Chemical Communications
Volume	56
Issue number	65
Early online date	08 Jul 2020
DOIs	https://doi.org/10.1039/D0CC03721D
Publication status	Published - 21 Aug 2020

Bibliographical note

This is an interdisciplinary research in collaboration with organic chemist from Zhejiang Univ. China.

The work was conducted by a visiting PhD student from Zhejiang University.

Chem Comm is a peer-reviewed journal with Impact Factor of 6.2

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10.1039/D0CC03721DLicence: Unspecified

The Mutagenesis of a Single Site for Enhancing or Reversing the Enantio- or Regiopreference of Cyclohexanone Monooxygenases
Copyright 2020 Royal Society of Chemistry. This work is made available online in accordance with the publisher’s policies. Please refer to any applicable terms of use of the publisher.
Accepted author manuscript, 335 KBLicence: Unspecified

Meilan Huang
- m.huangqub.acuk
- School of Chemistry and Chemical Engineering - Senior Lecturer
- Theoretical and Applied Catalysis
Person: Academic

Cite this

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title = "The mutagenesis of a single site for enhancing or reversing the enantio- or regiopreference of cyclohexanone monooxygenases",

abstract = "The mutagenesis of a “second sphere” switch residue of CHMOAcineto could control its enantio- and regiopreference. Replacing phenylalanine (F) at position 277 of CHMOAcineto into larger tryptophan (W) enabled a significant enhancement of enantio- or regioselectivity toward structurally diverse substrates, moreover, a complete reversal of enantio- or regiopreference was realized by mutating F277 into a range of smaller amino acids (A/C/D/E/G/H/I/K/L/M/N/P/Q/R/S/T/V).",

author = "Yujing Hu and Weihua Xu and Chenggong Hui and Jian Xu and Xianfu Lin and Meilan Huang and Qi Wu",

note = "This is an interdisciplinary research in collaboration with organic chemist from Zhejiang Univ. China. The work was conducted by a visiting PhD student from Zhejiang University. Chem Comm is a peer-reviewed journal with Impact Factor of 6.2 ",

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T1 - The mutagenesis of a single site for enhancing or reversing the enantio- or regiopreference of cyclohexanone monooxygenases

AU - Hu, Yujing

AU - Xu, Weihua

AU - Hui, Chenggong

AU - Xu, Jian

AU - Lin, Xianfu

AU - Huang, Meilan

AU - Wu, Qi

N1 - This is an interdisciplinary research in collaboration with organic chemist from Zhejiang Univ. China. The work was conducted by a visiting PhD student from Zhejiang University. Chem Comm is a peer-reviewed journal with Impact Factor of 6.2

PY - 2020/8/21

Y1 - 2020/8/21

N2 - The mutagenesis of a “second sphere” switch residue of CHMOAcineto could control its enantio- and regiopreference. Replacing phenylalanine (F) at position 277 of CHMOAcineto into larger tryptophan (W) enabled a significant enhancement of enantio- or regioselectivity toward structurally diverse substrates, moreover, a complete reversal of enantio- or regiopreference was realized by mutating F277 into a range of smaller amino acids (A/C/D/E/G/H/I/K/L/M/N/P/Q/R/S/T/V).

AB - The mutagenesis of a “second sphere” switch residue of CHMOAcineto could control its enantio- and regiopreference. Replacing phenylalanine (F) at position 277 of CHMOAcineto into larger tryptophan (W) enabled a significant enhancement of enantio- or regioselectivity toward structurally diverse substrates, moreover, a complete reversal of enantio- or regiopreference was realized by mutating F277 into a range of smaller amino acids (A/C/D/E/G/H/I/K/L/M/N/P/Q/R/S/T/V).

U2 - 10.1039/D0CC03721D

DO - 10.1039/D0CC03721D

M3 - Article

SN - 1359-7345

VL - 56

SP - 9356

EP - 9359

JO - Chemical Communications

JF - Chemical Communications

IS - 65

ER -

The mutagenesis of a single site for enhancing or reversing the enantio- or regiopreference of cyclohexanone monooxygenases

Abstract

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Meilan Huang

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