The mutagenesis of a single site for enhancing or reversing the enantio- or regiopreference of cyclohexanone monooxygenases

Yujing Hu, Weihua Xu, Chenggong Hui, Jian Xu, Xianfu Lin, Meilan Huang, Qi Wu

Research output: Contribution to journalArticlepeer-review

Abstract

The mutagenesis of a “second sphere” switch residue of CHMOAcineto could control its enantio- and regiopreference. Replacing phenylalanine (F) at position 277 of CHMOAcineto into larger tryptophan (W) enabled a significant enhancement of enantio- or regioselectivity toward structurally diverse substrates, moreover, a complete reversal of enantio- or regiopreference was realized by mutating F277 into a range of smaller amino acids (A/C/D/E/G/H/I/K/L/M/N/P/Q/R/S/T/V).
Original languageEnglish
Pages (from-to)9356-9359
Number of pages4
JournalChemical Communications
Volume56
Issue number65
Early online date08 Jul 2020
Publication statusPublished - 2020

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