The Phosphorylation Mechanism of Mevalonate Diphosphate Decarboxylase, a QM/MM Study

James McClory, Chenggong Hui, Jian Zhang, Meilan Huang

Research output: Contribution to journalArticle

1 Citation (Scopus)

Abstract

Mevalonate diphosphate decarboxylase (MDD) catalyses a crucial step of the mevalonate pathway via Mg2+-ATP dependent phosphorylation and decarboxylation reactions to ultimately produce isopentenyl diphosphate, the precursor of isoprenoids, which is essential to bacteria functions and offers ideal building blocks for the biosynthesis of isopentenols. However, the metal ion(s) in MDD has not been unambiguously resolved, which limits the understanding of the catalytic mechanism and the exploitation of the enzyme for the development of antibacterial therapies or the mevalonate metabolic pathway for the biosynthesise of biofuels. Here by analogizing the structurally related kinases and molecular dynamics simulations, we constructed a model of the MDD-substrate-ATP-Mg2+ complex and propose MDD requires two Mg2+ ions for maintaining a catalytically active conformation. Subsequent QM/MM studies indicate MDD catalyses the phosphorylation of its substrate mevalonate diphosphate (MVAPP) via a direct phosphorylation reaction, instead of the previously assumed catalytic base mechanism. The results here would shed light for the active conformation of MDD related enzymes and their catalytic mechanisms and therefore be useful for developing novel antimicrobial therapies or reconstructing mevalonate metabolic pathway for the biosynthesis of biofuels.
Original languageEnglish
JournalOrganic and Biomolecular Chemistry
Early online date11 Dec 2019
DOIs
Publication statusEarly online date - 11 Dec 2019

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