Abstract
Selenium (Se) is an essential component for animals and human beings. The chemoprotective role of Se, via the regulation of the cell cycle, stimulation of apoptosis and activation of some cytokines among others, is well known; however, the comprehensive effects of Se on the expression of IGF-IR and its regulation of apoptosis have not been investigated. Thus the aim of this study was to report on the effects that different concentrations of Se extert on body weight, blood serum IGF-IR levels and histopathology in mice; and on IGF-IR expression, proliferation and apoptosis in mouse osteoblasts. In vivo experiments showed a significant decrease in body weight, serum levels of IGF-IR and prominent toxicant effects on the liver, kidney, heart and spleen following the administration of defined concentrations of Se for 30 d. However, moderate levels (0.1 mg/kg) of Se gradually improved weight and serum IGF-IR. In vitro osteoblast experiments revealed that at concentrations of 5 × 10-6 and 10-5 mol/L Se, MTT activity decreased in comparison with control cells. Cell cycle, TEM and caspase-3 activity supported these observations including an increase in the sub-G1 phase and notable apoptosis in osteoblasts, along with a decrease in the expression of mRNA and protein levels of IGF-IR. Moreover, the MTT activity, mRNA and protein levels of IGF-IR in osteoblasts were decreased and caspase-3 activity was increased in siRNA groups as compared with non-siRNA groups. These data suggest that Se significantly affects IGF-IR expression, and that it contributes to the proliferation and regulation of apoptosis in osteoblasts.
Original language | English |
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Pages (from-to) | 2158-2164 |
Number of pages | 7 |
Journal | Chemosphere |
Volume | 144 |
Early online date | 17 Nov 2015 |
DOIs | |
Publication status | Published - 01 Feb 2016 |
Externally published | Yes |
Bibliographical note
Funding Information:This work was supported by the Natural Scientific Funds of China through projects NSFC (No. 30571362 , No. 30972230 and No. 31272623 ).
Publisher Copyright:
© 2015 Elsevier Ltd.
Keywords
- Apoptosis
- IGF-IR
- Mice
- Osteoblast
- Proliferation
- Selenium
ASJC Scopus subject areas
- Environmental Engineering
- Environmental Chemistry
- General Chemistry
- Pollution
- Health, Toxicology and Mutagenesis