The rumen microbiome: an underexplored resource for novel antimicrobial discovery

Linda Oyama, Susan Girdwood, Alan Cookson, Narcis Fernandez-Fuentes, Florence Prive, Hannah Vallin, Toby Wilkinson, Peter Golyshin, Olga Golyshina, Ralf Mikut, Kai Hilpert, J Richards, Wooton Mandy, Joan Edwards, Marc Marcesa, J Perrier, Fionnuala Lundy, Y Luo, M Zhou, Matthias HessHilario Mantovani, Chris Creevey, Sharon Huws

Research output: Contribution to journalArticle

12 Citations (Scopus)
228 Downloads (Pure)

Abstract

Antimicrobial peptides (AMPs) are promising drug candidates to target multi-drug resistant bacteria. The rumen microbiome presents an underexplored resource for the discovery of novel microbial enzymes and metabolites, including AMPs. Using functional screening and computational approaches, we identified 181 potentially novel AMPs from a rumen bacterial metagenome. Here, we show that three of the selected AMPs (Lynronne-1, Lynronne-2 and Lynronne-3) were effective against numerous bacterial pathogens, including methicillin-resistant Staphylococcus aureus (MRSA). No decrease in MRSA susceptibility was observed after 25 days of sub-lethal exposure to these AMPs. The AMPs bound preferentially to bacterial membrane lipids and induced membrane permeability leading to cytoplasmic leakage. Topical administration of Lynronne-1 (10% w/v) to a mouse model of MRSA wound infection elicited a significant reduction in bacterial counts, which was comparable to treatment with 2% mupirocin ointment. Our findings indicate that the rumen microbiome may provide viable alternative antimicrobials for future therapeutic application.
Original languageEnglish
Pages (from-to)1-10
JournalNPJ Biofilms and Microbiomes
Volume3
Issue number33
Early online date01 Dec 2017
DOIs
Publication statusEarly online date - 01 Dec 2017

Fingerprint Dive into the research topics of 'The rumen microbiome: an underexplored resource for novel antimicrobial discovery'. Together they form a unique fingerprint.

  • Cite this

    Oyama, L., Girdwood, S., Cookson, A., Fernandez-Fuentes, N., Prive, F., Vallin, H., Wilkinson, T., Golyshin, P., Golyshina, O., Mikut, R., Hilpert, K., Richards, J., Mandy, W., Edwards, J., Marcesa, M., Perrier, J., Lundy, F., Luo, Y., Zhou, M., ... Huws, S. (2017). The rumen microbiome: an underexplored resource for novel antimicrobial discovery. NPJ Biofilms and Microbiomes, 3(33), 1-10. https://doi.org/10.1038/s41522-017-0042-1