The topography of DNA methylation in the non-neoplastic colonic mucosa surrounding colorectal cancers

Manish Mani Subramaniam, Marie Loh, Jason Yongsheng Chan, Natalia Liem, Pei Li Lim, Yong Wei Peng, Xn Yii Lim, Khay Guan Yeoh, Barry Iacopetta, Richie Soong, Manuel Salto-Tellez

Research output: Contribution to journalArticle

4 Citations (Scopus)

Abstract

The degree of gene hypermethylation in non-neoplastic colonic mucosa (NNCM) is a potentially important event in the development of colorectal cancer (CRC), particularly for the subgroup with a CpG island methylator phenotype (CIMP). In this study, we aimed to use an unbiased and high-throughput approach to evaluate the topography of DNA methylation in the non-neoplastic colonic mucosa (NNCM) surrounding colorectal cancer (CRC). A total of 61 tissue samples comprising 53 NNCM and 8 tumor samples were obtained from hemicolectomy specimens of two CRC patients (Cases 1 and 2). NNCM was stripped from the underlying colonic wall and samples taken at varying distances from the tumor. The level of DNA methylation in NNCM and tumor tissues was assessed at 1,505 CpG sites in 807 cancer-related genes using Illumina GoldenGate® methylation arrays. Case 1 tumor showed significantly higher levels of methylation compared to surrounding NNCM samples (P?
Original languageEnglish
Pages (from-to)98-108
Number of pages11
JournalMolecular Carcinogenesis
Volume53
Issue number2
DOIs
Publication statusPublished - 21 Aug 2012

Bibliographical note

Copyright © 2012 Wiley Periodicals, Inc.

ASJC Scopus subject areas

  • Cancer Research
  • Molecular Biology

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