The topography of DNA methylation in the non-neoplastic colonic mucosa surrounding colorectal cancers

Manish Mani Subramaniam; Marie Loh; Jason Yongsheng Chan; Natalia Liem; Pei Li Lim; Yong Wei Peng; Xn Yii Lim; Khay Guan Yeoh; Barry Iacopetta; Richie Soong; Manuel Salto-Tellez

doi:10.1002/mc.21951

The topography of DNA methylation in the non-neoplastic colonic mucosa surrounding colorectal cancers

Manish Mani Subramaniam
, Marie Loh
, Jason Yongsheng Chan
, Natalia Liem
, Pei Li Lim
, Yong Wei Peng
, Xn Yii Lim
, Khay Guan Yeoh
, Barry Iacopetta
, Richie Soong
, Manuel Salto-Tellez

Research output: Contribution to journal › Article › peer-review

6 Citations (Scopus)

Abstract

The degree of gene hypermethylation in non-neoplastic colonic mucosa (NNCM) is a potentially important event in the development of colorectal cancer (CRC), particularly for the subgroup with a CpG island methylator phenotype (CIMP). In this study, we aimed to use an unbiased and high-throughput approach to evaluate the topography of DNA methylation in the non-neoplastic colonic mucosa (NNCM) surrounding colorectal cancer (CRC). A total of 61 tissue samples comprising 53 NNCM and 8 tumor samples were obtained from hemicolectomy specimens of two CRC patients (Cases 1 and 2). NNCM was stripped from the underlying colonic wall and samples taken at varying distances from the tumor. The level of DNA methylation in NNCM and tumor tissues was assessed at 1,505 CpG sites in 807 cancer-related genes using Illumina GoldenGate® methylation arrays. Case 1 tumor showed significantly higher levels of methylation compared to surrounding NNCM samples (P?

Original language	English
Pages (from-to)	98-108
Number of pages	11
Journal	Molecular Carcinogenesis
Volume	53
Issue number	2
DOIs	https://doi.org/10.1002/mc.21951
Publication status	Published - 21 Aug 2012

Bibliographical note

UN SDGs

This output contributes to the following UN Sustainable Development Goals (SDGs)

SDG 3 Good Health and Well-being

ASJC Scopus subject areas

Cancer Research
Molecular Biology

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10.1002/mc.21951

Cite this

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title = "The topography of DNA methylation in the non-neoplastic colonic mucosa surrounding colorectal cancers",

abstract = "The degree of gene hypermethylation in non-neoplastic colonic mucosa (NNCM) is a potentially important event in the development of colorectal cancer (CRC), particularly for the subgroup with a CpG island methylator phenotype (CIMP). In this study, we aimed to use an unbiased and high-throughput approach to evaluate the topography of DNA methylation in the non-neoplastic colonic mucosa (NNCM) surrounding colorectal cancer (CRC). A total of 61 tissue samples comprising 53 NNCM and 8 tumor samples were obtained from hemicolectomy specimens of two CRC patients (Cases 1 and 2). NNCM was stripped from the underlying colonic wall and samples taken at varying distances from the tumor. The level of DNA methylation in NNCM and tumor tissues was assessed at 1,505 CpG sites in 807 cancer-related genes using Illumina GoldenGate{\textregistered} methylation arrays. Case 1 tumor showed significantly higher levels of methylation compared to surrounding NNCM samples (P?",

author = "Subramaniam, \{Manish Mani\} and Marie Loh and Chan, \{Jason Yongsheng\} and Natalia Liem and Lim, \{Pei Li\} and Peng, \{Yong Wei\} and Lim, \{Xn Yii\} and Yeoh, \{Khay Guan\} and Barry Iacopetta and Richie Soong and Manuel Salto-Tellez",

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year = "2012",

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doi = "10.1002/mc.21951",

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AU - Subramaniam, Manish Mani

AU - Loh, Marie

AU - Chan, Jason Yongsheng

AU - Liem, Natalia

AU - Lim, Pei Li

AU - Peng, Yong Wei

AU - Lim, Xn Yii

AU - Yeoh, Khay Guan

AU - Iacopetta, Barry

AU - Soong, Richie

AU - Salto-Tellez, Manuel

PY - 2012/8/21

Y1 - 2012/8/21

N2 - The degree of gene hypermethylation in non-neoplastic colonic mucosa (NNCM) is a potentially important event in the development of colorectal cancer (CRC), particularly for the subgroup with a CpG island methylator phenotype (CIMP). In this study, we aimed to use an unbiased and high-throughput approach to evaluate the topography of DNA methylation in the non-neoplastic colonic mucosa (NNCM) surrounding colorectal cancer (CRC). A total of 61 tissue samples comprising 53 NNCM and 8 tumor samples were obtained from hemicolectomy specimens of two CRC patients (Cases 1 and 2). NNCM was stripped from the underlying colonic wall and samples taken at varying distances from the tumor. The level of DNA methylation in NNCM and tumor tissues was assessed at 1,505 CpG sites in 807 cancer-related genes using Illumina GoldenGate® methylation arrays. Case 1 tumor showed significantly higher levels of methylation compared to surrounding NNCM samples (P?

AB - The degree of gene hypermethylation in non-neoplastic colonic mucosa (NNCM) is a potentially important event in the development of colorectal cancer (CRC), particularly for the subgroup with a CpG island methylator phenotype (CIMP). In this study, we aimed to use an unbiased and high-throughput approach to evaluate the topography of DNA methylation in the non-neoplastic colonic mucosa (NNCM) surrounding colorectal cancer (CRC). A total of 61 tissue samples comprising 53 NNCM and 8 tumor samples were obtained from hemicolectomy specimens of two CRC patients (Cases 1 and 2). NNCM was stripped from the underlying colonic wall and samples taken at varying distances from the tumor. The level of DNA methylation in NNCM and tumor tissues was assessed at 1,505 CpG sites in 807 cancer-related genes using Illumina GoldenGate® methylation arrays. Case 1 tumor showed significantly higher levels of methylation compared to surrounding NNCM samples (P?

U2 - 10.1002/mc.21951

DO - 10.1002/mc.21951

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ER -

The topography of DNA methylation in the non-neoplastic colonic mucosa surrounding colorectal cancers

Abstract

Bibliographical note

UN SDGs

ASJC Scopus subject areas

Access to Document

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