The transcriptome of mouse central nervous system myelin

Sudhir Thakurela, Angela Garding, Ramona B. Jung, Christina Müller, Sandra Goebbels, Robin White, Hauke B. Werner, Vijay K. Tiwari

Research output: Chapter in Book/Report/Conference proceedingChapter

21 Citations (Scopus)

Abstract

Rapid nerve conduction in the CNS is facilitated by insulation of axons with myelin, a specialized oligodendroglial compartment distant from the cell body. Myelin is turned over and adapted throughout life; however, the molecular and cellular basis of myelin dynamics remains elusive. Here we performed a comprehensive transcriptome analysis (RNA-seq) of myelin biochemically purified from mouse brains at various ages and find a surprisingly large pool of transcripts enriched in myelin. Further computational analysis showed that the myelin transcriptome is closely related to the myelin proteome but clearly distinct from the transcriptomes of oligodendrocytes and brain tissues, suggesting a highly selective incorporation of mRNAs into the myelin compartment. The mRNA-pool in myelin displays maturation-dependent dynamic changes of composition, abundance, and functional associations; however ageing-dependent changes after 6 months were minor. We suggest that this transcript pool enables myelin turnover and the local adaptation of individual pre-existing myelin sheaths.
Original languageEnglish
Title of host publicationScientific Reports
PublisherNature Publishing Group
ISBN (Print)2045-2322 (Electronic)2045-2322 (Linking)
DOIs
Publication statusPublished - 13 May 2016

Publication series

NameScientific Reports
Volume6

Fingerprint

Myelin Sheath
Transcriptome
Central Nervous System
Messenger RNA
Neural Conduction
Oligodendroglia
Brain
Gene Expression Profiling
Proteome
Axons
RNA

Cite this

Thakurela, S., Garding, A., Jung, R. B., Müller, C., Goebbels, S., White, R., ... Tiwari, V. K. (2016). The transcriptome of mouse central nervous system myelin. In Scientific Reports (Scientific Reports; Vol. 6). Nature Publishing Group. https://doi.org/10.1038/srep25828
Thakurela, Sudhir ; Garding, Angela ; Jung, Ramona B. ; Müller, Christina ; Goebbels, Sandra ; White, Robin ; Werner, Hauke B. ; Tiwari, Vijay K. / The transcriptome of mouse central nervous system myelin. Scientific Reports. Nature Publishing Group, 2016. (Scientific Reports).
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Thakurela, S, Garding, A, Jung, RB, Müller, C, Goebbels, S, White, R, Werner, HB & Tiwari, VK 2016, The transcriptome of mouse central nervous system myelin. in Scientific Reports. Scientific Reports, vol. 6, Nature Publishing Group. https://doi.org/10.1038/srep25828

The transcriptome of mouse central nervous system myelin. / Thakurela, Sudhir; Garding, Angela; Jung, Ramona B.; Müller, Christina; Goebbels, Sandra; White, Robin; Werner, Hauke B.; Tiwari, Vijay K.

Scientific Reports. Nature Publishing Group, 2016. (Scientific Reports; Vol. 6).

Research output: Chapter in Book/Report/Conference proceedingChapter

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Thakurela S, Garding A, Jung RB, Müller C, Goebbels S, White R et al. The transcriptome of mouse central nervous system myelin. In Scientific Reports. Nature Publishing Group. 2016. (Scientific Reports). https://doi.org/10.1038/srep25828