The Viral protein Corona Directs Viral Pathogenesis and Amyloid Aggregation

Kariem Ezzat; Maria Pernemalm; Sandra Palsson; Thomas C. Roberts; Peter Jarver; Aleksandra Dondalska; Burcu  Bestas; Michal J. Sobkowiak; Bettina  Levanen; Magnus Skold; Elizabeth A. Thompson; Osama Saher; Otto K. Kari; Tatu Lajunen; Eva Sverremark Ekstrom; Caroline Nilsson; Yevheniia Ishchenko; Tarja Malm; Matthew J.A. Wood; Ultan Power; Sergej Masich; Anders Linden; Johan K.  Sandberg; Janne  Lehtio; Anna-Lena Spetz; Samir EL Andaloussi

doi:10.1038/s41467-019-10192-2

The Viral protein Corona Directs Viral Pathogenesis and Amyloid Aggregation

Kariem Ezzat, Maria Pernemalm, Sandra Palsson, Thomas C. Roberts, Peter Jarver, Aleksandra Dondalska, Burcu Bestas, Michal J. Sobkowiak, Bettina Levanen, Magnus Skold, Elizabeth A. Thompson, Osama Saher, Otto K. Kari, Tatu Lajunen, Eva Sverremark Ekstrom, Caroline Nilsson, Yevheniia Ishchenko, Tarja Malm, Matthew J.A. Wood, Ultan PowerSergej Masich, Anders Linden, Johan K. Sandberg, Janne Lehtio, Anna-Lena Spetz, Samir EL Andaloussi

School of Medicine, Dentistry and Biomedical Sciences

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Abstract

Artificial nanoparticles accumulate a protein corona layer in biological fluids, which significantly influences their bioactivity. As nanosized obligate intracellular parasites, viruses share many biophysical properties with artificial nanoparticles in extracellular environments and here we show that respiratory syncytial virus (RSV) and herpes simplex virus 1 (HSV-1) accumulate a rich and distinctive protein corona in different biological fluids. Moreover, we show that corona pre-coating differentially affects viral infectivity and immune cell activation. Additionally, we demonstrate that viruses bind amyloidogenic peptides in their corona and catalyze amyloid formation via surface-assisted heterogeneous nucleation. Importantly, we show that HSV-1 catalyzes the aggregation of the amyloid beta peptide (Aβ42), a major constituent of amyloid plaques in Alzheimer's disease, in-vitro and in animal models. Our results highlight the viral protein corona as an acquired structural layer that is critical for viral-host interactions and illustrate a mechanistic convergence between viral and amyloid pathologies.

Original language	English
Article number	2331
Journal	Nature Communications
Volume	10
DOIs	https://doi.org/10.1038/s41467-019-10192-2
Publication status	Published - 27 May 2019

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10.1038/s41467-019-10192-2Licence: CC BY

The viral protein corona directs viral pathogenesis and amyloid aggregation
Copyright 2019 the authors. This is an open access article published under a Creative Commons Attribution License (https://creativecommons.org/licenses/by/4.0/), which permits unrestricted use, distribution and reproduction in any medium, provided the author and source are cited.
Final published version, 4.23 MBLicence: CC BY

Ultan Power
- u.powerqub.acuk
- School of Medicine, Dentistry and Biomedical Sciences - Professor
- Wellcome Wolfson Institute for Experimental Medicine
Person: Academic

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Ezzat, K., Pernemalm, M., Palsson, S., Roberts, T. C., Jarver, P., Dondalska, A., Bestas, B., Sobkowiak, M. J., Levanen, B., Skold, M., Thompson, E. A., Saher, O., Kari, O. K., Lajunen, T., Ekstrom, E. S., Nilsson, C., Ishchenko, Y., Malm, T., Wood, M. J. A., ... Andaloussi, S. EL. (2019). The Viral protein Corona Directs Viral Pathogenesis and Amyloid Aggregation. Nature Communications, 10, Article 2331. https://doi.org/10.1038/s41467-019-10192-2

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title = "The Viral protein Corona Directs Viral Pathogenesis and Amyloid Aggregation",

abstract = "Artificial nanoparticles accumulate a protein corona layer in biological fluids, which significantly influences their bioactivity. As nanosized obligate intracellular parasites, viruses share many biophysical properties with artificial nanoparticles in extracellular environments and here we show that respiratory syncytial virus (RSV) and herpes simplex virus 1 (HSV-1) accumulate a rich and distinctive protein corona in different biological fluids. Moreover, we show that corona pre-coating differentially affects viral infectivity and immune cell activation. Additionally, we demonstrate that viruses bind amyloidogenic peptides in their corona and catalyze amyloid formation via surface-assisted heterogeneous nucleation. Importantly, we show that HSV-1 catalyzes the aggregation of the amyloid beta peptide (Aβ42), a major constituent of amyloid plaques in Alzheimer's disease, in-vitro and in animal models. Our results highlight the viral protein corona as an acquired structural layer that is critical for viral-host interactions and illustrate a mechanistic convergence between viral and amyloid pathologies.",

author = "Kariem Ezzat and Maria Pernemalm and Sandra Palsson and Roberts, {Thomas C.} and Peter Jarver and Aleksandra Dondalska and Burcu Bestas and Sobkowiak, {Michal J.} and Bettina Levanen and Magnus Skold and Thompson, {Elizabeth A.} and Osama Saher and Kari, {Otto K.} and Tatu Lajunen and Ekstrom, {Eva Sverremark} and Caroline Nilsson and Yevheniia Ishchenko and Tarja Malm and Wood, {Matthew J.A.} and Ultan Power and Sergej Masich and Anders Linden and Sandberg, {Johan K.} and Janne Lehtio and Anna-Lena Spetz and Andaloussi, {Samir EL}",

year = "2019",

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doi = "10.1038/s41467-019-10192-2",

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Ezzat, K, Pernemalm, M, Palsson, S, Roberts, TC, Jarver, P, Dondalska, A, Bestas, B, Sobkowiak, MJ, Levanen, B, Skold, M, Thompson, EA, Saher, O, Kari, OK, Lajunen, T, Ekstrom, ES, Nilsson, C, Ishchenko, Y, Malm, T, Wood, MJA, Power, U, Masich, S, Linden, A, Sandberg, JK, Lehtio, J, Spetz, A-L & Andaloussi, SEL 2019, 'The Viral protein Corona Directs Viral Pathogenesis and Amyloid Aggregation', Nature Communications, vol. 10, 2331. https://doi.org/10.1038/s41467-019-10192-2

TY - JOUR

T1 - The Viral protein Corona Directs Viral Pathogenesis and Amyloid Aggregation

AU - Ezzat, Kariem

AU - Pernemalm, Maria

AU - Palsson, Sandra

AU - Roberts, Thomas C.

AU - Jarver, Peter

AU - Dondalska, Aleksandra

AU - Bestas, Burcu

AU - Sobkowiak, Michal J.

AU - Levanen, Bettina

AU - Skold, Magnus

AU - Thompson, Elizabeth A.

AU - Saher, Osama

AU - Kari, Otto K.

AU - Lajunen, Tatu

AU - Ekstrom, Eva Sverremark

AU - Nilsson, Caroline

AU - Ishchenko, Yevheniia

AU - Malm, Tarja

AU - Wood, Matthew J.A.

AU - Power, Ultan

AU - Masich, Sergej

AU - Linden, Anders

AU - Sandberg, Johan K.

AU - Lehtio, Janne

AU - Spetz, Anna-Lena

AU - Andaloussi, Samir EL

PY - 2019/5/27

Y1 - 2019/5/27

N2 - Artificial nanoparticles accumulate a protein corona layer in biological fluids, which significantly influences their bioactivity. As nanosized obligate intracellular parasites, viruses share many biophysical properties with artificial nanoparticles in extracellular environments and here we show that respiratory syncytial virus (RSV) and herpes simplex virus 1 (HSV-1) accumulate a rich and distinctive protein corona in different biological fluids. Moreover, we show that corona pre-coating differentially affects viral infectivity and immune cell activation. Additionally, we demonstrate that viruses bind amyloidogenic peptides in their corona and catalyze amyloid formation via surface-assisted heterogeneous nucleation. Importantly, we show that HSV-1 catalyzes the aggregation of the amyloid beta peptide (Aβ42), a major constituent of amyloid plaques in Alzheimer's disease, in-vitro and in animal models. Our results highlight the viral protein corona as an acquired structural layer that is critical for viral-host interactions and illustrate a mechanistic convergence between viral and amyloid pathologies.

AB - Artificial nanoparticles accumulate a protein corona layer in biological fluids, which significantly influences their bioactivity. As nanosized obligate intracellular parasites, viruses share many biophysical properties with artificial nanoparticles in extracellular environments and here we show that respiratory syncytial virus (RSV) and herpes simplex virus 1 (HSV-1) accumulate a rich and distinctive protein corona in different biological fluids. Moreover, we show that corona pre-coating differentially affects viral infectivity and immune cell activation. Additionally, we demonstrate that viruses bind amyloidogenic peptides in their corona and catalyze amyloid formation via surface-assisted heterogeneous nucleation. Importantly, we show that HSV-1 catalyzes the aggregation of the amyloid beta peptide (Aβ42), a major constituent of amyloid plaques in Alzheimer's disease, in-vitro and in animal models. Our results highlight the viral protein corona as an acquired structural layer that is critical for viral-host interactions and illustrate a mechanistic convergence between viral and amyloid pathologies.

U2 - 10.1038/s41467-019-10192-2

DO - 10.1038/s41467-019-10192-2

M3 - Article

SN - 2041-1723

VL - 10

JO - Nature Communications

JF - Nature Communications

M1 - 2331

ER -

The Viral protein Corona Directs Viral Pathogenesis and Amyloid Aggregation

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