Thermoresponsive Polymer Micropatterns Fabricated by Dip-Pen Nanolithography for a Highly Controllable Substrate with Potential Cellular Applications

Stacey Laing, Raffaella Suriano, Dimitrios A. Lamprou, Carol-Anne Smith, Matthew J. Dalby, Samuel Mabbott, Karen Faulds, Duncan Graham*

*Corresponding author for this work

Research output: Contribution to journalArticle

8 Citations (Scopus)

Abstract

We report a novel approach for patterning thermoresponsive hydrogels based on N,N-diethylacrylamide (DEAAm) and bifunctional Jeffamine ED-600 by dip-pen nanolithography (DPN). The direct writing of micron-sized thermoresponsive polymer spots was achieved with efficient control over feature size. A Jeffamine-based ink prepared through the combination of organic polymers, such as DEAAm, in an inorganic silica network was used to print thermosensitive arrays on a thiol-silanized silicon oxide substrate. The use of a Jeffamine hydrogel, acting as a carrier matrix, allowed a reduction in the evaporation of ink molecules with high volatility, such as DEAAm, and facilitated the transfer of ink from tip to substrate. The thermoresponsive behavior of polymer arrays which swell/deswell in aqueous solution in response to a change in temperature was successfully characterized by atomic force microscopy (AFM) and Raman spectroscopy: a thermally induced change in height and hydration state was observed, respectively. Finally, we demonstrate that cells can adhere to and interact with these dynamic features and exhibit a change in behavior when cultured on the substrates above and below the transition temperature of the Jeffamine/DEAAm thermoresponsive hydrogels. This demonstrates the potential of these micropatterned hydrogels to act as a controllable surface for cell growth.

Original languageEnglish
Pages (from-to)24844-24852
Number of pages9
JournalACS Applied Materials and Interfaces
Volume8
Issue number37
Early online date30 Aug 2016
DOIs
Publication statusPublished - 21 Sep 2016
Externally publishedYes

Keywords

  • atomic force microscopy
  • dip-pen nanolithography
  • polymer arrays
  • smart hydrogel structures
  • thermoresponsive polymers

ASJC Scopus subject areas

  • Materials Science(all)

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